Paper · 2023 · International Journal of Farm Sciences

In vitro bio-efficacy of different antibiotics and bio-agents against bacterial canker (Xanthomonas axonopodis pv citri) of acid lime

Raj Kumar; MH Shete; VK Garande

14
claims
33
verbatim quotes
0
citations
3
research areas
✓
Open access · cc-by

Abstract

Four antibiotics in combination with antibacterial chemicals and two bio-agents viz Pseudomonas fluorescens and Bacillus subtilis, were evaluated in vitro against Xanthomonas axonopodis pv citri (Hasse). The results revealed that among the different antibiotics, in combination with antibacterial chemicals, streptocycline (100 ppm) + copper oxychloride (0.25%) showed higher inhibition zone (10.0 mm) as compared to kasugamycin (0.15%) (8.6 mm) and validamycin (0.2%) + copper oxychloride (0.25%) (8.8 mm). In vitro study of bio-agents by dual culture method revealed that P fluorescence showed maximum inhibition zone (97.2%) followed by B subtilis (92%). In vivo evaluation of antibiotics in combination with antibacterial chemicals and bio-agents revealed that the minimum disease intensity of 19.53 and 19.63 per cent was recorded in streptocycline (100 ppm) + copper oxychloride (0.25%) and streptocycline (100 ppm) + copper hydroxide (0.5%) with 40.41 and 39.46 per cent disease control respectively. Maximum disease intensity was recorded in control (32.50%) with 0.0 per cent disease control followed by B subtilis (5 g/l) (27.41%) and P fluorescence (5 g/l) (26.43%) with disease control of 16.81 and 19.20 per cent respectively.

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Claims 14

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Streptocycline and copper oxychloride gave a 10.0 mm inhibition zone in vitro.

Taxon
acid limet0
Treatment
streptocycline (100 ppm) + copper oxychloride (0.25%)e0
Outcome
inhibition zonee0
Direction
highere0
Value
10.0 mme0

Not reportedComparator

Verbatim

e0

“streptocycline (100 ppm) + copper oxychloride (0.25%) showed higher inhibition zone (10.0 mm)”

✓ located verbatim in the source · abstract, characters 308–401 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Kasugamycin produced an 8.6 mm inhibition zone.

Taxon
acid limet0
Treatment
kasugamycin (0.15%)e0
Outcome
inhibition zonee1
Value
8.6 mme0

Not reportedComparatorDirection

Verbatim

e0

“kasugamycin (0.15%) (8.6 mm)”

✓ located verbatim in the source · abstract, characters 417–445 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
e1

“streptocycline (100 ppm) + copper oxychloride (0.25%) showed higher inhibition zone (10.0 mm)”

✓ located verbatim in the source · abstract, characters 308–401 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Validamycin plus copper oxychloride produced an 8.8 mm inhibition zone.

Taxon
acid limet0
Treatment
validamycin (0.2%) + copper oxychloride (0.25%)e0
Outcome
inhibition zonee1
Value
8.8 mme0

Not reportedComparatorDirection

Verbatim

e0

“validamycin (0.2%) + copper oxychloride (0.25%) (8.8 mm)”

✓ located verbatim in the source · abstract, characters 450–506 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
e1

“streptocycline (100 ppm) + copper oxychloride (0.25%) showed higher inhibition zone (10.0 mm)”

✓ located verbatim in the source · abstract, characters 308–401 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

P fluorescence gave a maximum 97.2% inhibition zone.

Taxon
acid limet0
Treatment
P fluorescencee0
Interactor
P fluorescencee0biocontrol_agent
Outcome
inhibition zonee0
Direction
maximume0
Value
97.2%e0

Not reportedComparator

Verbatim

e0

“P fluorescence showed maximum inhibition zone (97.2%)”

✓ located verbatim in the source · abstract, characters 574–627 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

B subtilis gave a 92% inhibition zone.

Taxon
acid limet0
Treatment
B subtilise0
Interactor
B subtilisBacillus subtilise0biocontrol_agent
Outcome
inhibition zonee1
Value
92%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“B subtilis (92%)”

✓ located verbatim in the source · abstract, characters 640–656 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
e1

“streptocycline (100 ppm) + copper oxychloride (0.25%) showed higher inhibition zone (10.0 mm)”

✓ located verbatim in the source · abstract, characters 308–401 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Streptocycline plus copper oxychloride produced 19.53% disease intensity.

Taxon
acid limet0
Treatment
streptocycline (100 ppm) + copper oxychloride (0.25%)e0
Outcome
disease intensitye0
Direction
minimume0
Value
19.53e0

Not reportedComparator

Verbatim

e0

“minimum disease intensity of 19.53 and 19.63 per cent was recorded in streptocycline (100 ppm) + copper oxychloride (0.25%) and streptocycline (100 ppm) + copper hydroxide (0.5%)”

✓ located verbatim in the source · abstract, characters 769–947 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Streptocycline plus copper hydroxide produced 19.63% disease intensity.

Taxon
acid limet0
Treatment
streptocycline (100 ppm) + copper hydroxide (0.5%)e0
Outcome
disease intensitye0
Direction
minimume0
Value
19.63 per cente0

Not reportedComparator

Verbatim

e0

“minimum disease intensity of 19.53 and 19.63 per cent was recorded in streptocycline (100 ppm) + copper oxychloride (0.25%) and streptocycline (100 ppm) + copper hydroxide (0.5%)”

✓ located verbatim in the source · abstract, characters 769–947 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Streptocycline plus copper oxychloride achieved 40.41% disease control.

Taxon
acid limet0
Outcome
disease controle0
Value
40.41e0

Not reportedTreatmentComparatorDirection

Verbatim

e0

“with 40.41 and 39.46 per cent disease control respectively”

✓ located verbatim in the source · abstract, characters 948–1006 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Streptocycline plus copper hydroxide achieved 39.46% disease control.

Taxon
acid limet0
Outcome
disease controle0
Value
39.46 per cente0

Not reportedTreatmentComparatorDirection

Verbatim

e0

“with 40.41 and 39.46 per cent disease control respectively”

✓ located verbatim in the source · abstract, characters 948–1006 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Untreated control had maximum disease intensity of 32.50%.

Taxon
acid limet0
Outcome
disease intensitye0
Direction
Maximume0
Value
32.50%e0

Not reportedTreatmentComparator

Verbatim

e0

“Maximum disease intensity was recorded in control (32.50%) with 0.0 per cent disease control”

✓ located verbatim in the source · abstract, characters 1008–1100 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

B subtilis treatment had disease intensity of 27.41%.

Taxon
acid limet0
Treatment
B subtilis (5 g/l)e0
Interactor
B subtilisBacillus subtilise0biocontrol_agent
Outcome
disease intensitye1
Value
27.41%e0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Bacillus subtilis
Bacillus subtilis · InteractorY tambe (original uploader) · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“B subtilis (5 g/l) (27.41%)”

✓ located verbatim in the source · abstract, characters 1113–1140 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
e1

“minimum disease intensity of 19.53 and 19.63 per cent was recorded in streptocycline (100 ppm) + copper oxychloride (0.25%) and streptocycline (100 ppm) + copper hydroxide (0.5%)”

✓ located verbatim in the source · abstract, characters 769–947 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

P fluorescence treatment had disease intensity of 26.43%.

Taxon
acid limet0
Treatment
P fluorescence (5 g/l)e0
Interactor
P fluorescencee0biocontrol_agent
Outcome
disease intensitye1
Value
26.43%e0

Not reportedComparatorDirection

Verbatim

e0

“P fluorescence (5 g/l) (26.43%)”

✓ located verbatim in the source · abstract, characters 1145–1176 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
e1

“minimum disease intensity of 19.53 and 19.63 per cent was recorded in streptocycline (100 ppm) + copper oxychloride (0.25%) and streptocycline (100 ppm) + copper hydroxide (0.5%)”

✓ located verbatim in the source · abstract, characters 769–947 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

B subtilis treatment controlled disease by 16.81%.

Taxon
acid limet0
Outcome
disease controle0
Value
16.81e0

Not reportedTreatmentComparatorDirection

Verbatim

e0

“disease control of 16.81 and 19.20 per cent respectively”

✓ located verbatim in the source · abstract, characters 1182–1238 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

P fluorescence treatment controlled disease by 19.20%.

Taxon
acid limet0
Outcome
disease controle0
Value
19.20 per cente0

Not reportedTreatmentComparatorDirection

Verbatim

e0

“disease control of 16.81 and 19.20 per cent respectively”

✓ located verbatim in the source · abstract, characters 1182–1238 · show in text ↑
t0

“acid lime”

✓ located verbatim in the source · title, characters 124–133 · show in text ↑