Abstract
Diploid Citrus × Poncirus hybrids have significantly contributed to citrus rootstock evaluation. In Türkiye, common sour orange rootstock is used in many different climates and soil conditions at almost 85% of citrus plantations, but this rootstock is susceptible to Citrus tristeza virus disease. This study was conducted to improve new rootstock genotypes by traditional hybridization. Common sour orange (CSO) and Common mandarin (Citrus deliciosa Ten.) (CM) were crossed with Troyer citrange (TC) while King mandarin (KM) was crossed with Carrizo citrange (CC). Embryos obtained from crosses were taken on 110, 120 and 130 days after artificial pollination (DAP), and were germinated on MT culture media. The embryos of 120 DAP of combinations had the highest germination rate within CSO×TC, 95.15%; CM×TC, 96.25%; KM×CC, 95.23%. The trifoliate rates for each combination at subculture (CSO×TC, 17.40%; CM×TC, 11.11%; KM×CC; 6.17%) were obtained from 110 DAP embryos. Survival rates of the genotypes were ranged between 72.13% and 90.28% in subculture and varied from 40.17% and 64.71% in the greenhouse. As a result of the ploidy analysis by flow cytometry, the nuclear DNA content of diploid genotypes were found between 0.78 pg/2C and 0.93 pg/2C. One of the genotypes derived from CM×TC hybridization on 120 DAP was determinated as a triploid plant.
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Claims 10
Claim · model-written summary
At 120 days after pollination, CSO×TC embryos had the highest germination rate, 95.15%.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryos of 120 DAP of combinations had the highest germination rate within CSO×TC, 95.15%; CM×TC, 96.25%; KM×CC, 95.23%.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
At 120 days after pollination, CM×TC embryos had the highest germination rate, 96.25%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryos of 120 DAP of combinations had the highest germination rate within CSO×TC, 95.15%; CM×TC, 96.25%; KM×CC, 95.23%.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
At 120 days after pollination, KM×CC embryos had the highest germination rate, 95.23%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The embryos of 120 DAP of combinations had the highest germination rate within CSO×TC, 95.15%; CM×TC, 96.25%; KM×CC, 95.23%.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
CSO×TC embryos collected at 110 days had a trifoliate rate of 17.40%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The trifoliate rates for each combination at subculture (CSO×TC, 17.40%; CM×TC, 11.11%; KM×CC; 6.17%) were obtained from 110 DAP embryos.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
CM×TC embryos collected at 110 days had a trifoliate rate of 11.11%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The trifoliate rates for each combination at subculture (CSO×TC, 17.40%; CM×TC, 11.11%; KM×CC; 6.17%) were obtained from 110 DAP embryos.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
KM×CC embryos collected at 110 days had a trifoliate rate of 6.17%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The trifoliate rates for each combination at subculture (CSO×TC, 17.40%; CM×TC, 11.11%; KM×CC; 6.17%) were obtained from 110 DAP embryos.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
Genotype survival in subculture ranged from 72.13% to 90.28%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Survival rates of the genotypes were ranged between 72.13% and 90.28% in subculture and varied from 40.17% and 64.71% in the greenhouse.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
Genotype survival in the greenhouse ranged from 40.17% to 64.71%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Survival rates of the genotypes were ranged between 72.13% and 90.28% in subculture and varied from 40.17% and 64.71% in the greenhouse.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
Diploid genotype nuclear DNA content ranged from 0.78 to 0.93 pg/2C.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As a result of the ploidy analysis by flow cytometry, the nuclear DNA content of diploid genotypes were found between 0.78 pg/2C and 0.93 pg/2C.”
t0“Citrus Rootstock Hybrids”
Claim · model-written summary
One CM×TC hybrid from 120-day embryos was identified as triploid.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One of the genotypes derived from CM×TC hybridization on 120 DAP was determinated as a triploid plant.”
t0“Citrus Rootstock Hybrids”