Abstract
Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium-sized evergreen tree found in the western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by overexploitation and loss of habitat. The development of microsatellite markers would help in understanding the genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced the partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. The probability of identity values ranged from 0.00329 to 0.30489. Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)
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Claims 6
Claim · model-written summary
Partial-genome sequencing identified 3725 microsatellites.
- Taxon
- G. indica
Garcinia indicae0 - Outcome
- microsatellitese0
- Value
- 3725e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we have sequenced the partial genome of G. indica and identified 3725 microsatellites.”
Claim · model-written summary
Marker polymorphism information content ranged from 0.718 to 0.968.
- Taxon
- Garcinia indicaGarcinia indicat0
- Outcome
- Polymorphism information content (PIC) valuese0
- Value
- 0.718 to 0.968e0
- Qualifier
- mean value of 0.922e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922.”
t0“Garcinia indica”
Claim · model-written summary
Markers had 3 to 33 alleles per locus.
- Taxon
- Garcinia indicaGarcinia indicat0
- Outcome
- Allele per locuse0
- Value
- 3 to 33 per locuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Allele per locus ranged from 3 to 33 per locus.”
t0“Garcinia indica”
Claim · model-written summary
Probability of identity ranged from 0.00329 to 0.30489.
- Taxon
- Garcinia indicaGarcinia indicat0
- Outcome
- probability of identity valuese0
- Value
- 0.00329 to 0.30489e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The probability of identity values ranged from 0.00329 to 0.30489.”
t0“Garcinia indica”
Claim · model-written summary
SSR primers had 12.5 % transferability in G. morella.
- Taxon
- G. morella
Garcinia morellae0 - Outcome
- transferabilitye0
- Value
- 12.5 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)”
Claim · model-written summary
SSR primers had 18.7% transferability in G. gummigutta.
Verbatim
e0“moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)”