Abstract
L. genotypes originating from the Balkan Peninsula region. In vitro shoot tips were briefly pre-cultured for 1 day at 23 °C in the dark on a medium containing 0.3 M sucrose and then embedded in calcium alginate gel within the wells of the aluminum cryo-plates. In the V cryo-plate protocol, dehydration was carried out at room temperature using the following vitrification solutions: original plant vitrification solution 2 (PVS2) and 90% PVS2 solution (for 20 and 40 min) and plant vitrification solution 3 (PVS3) (for 60 and 80 min). In the D cryo-plate protocol, desiccation was performed for 2, 2.5, or 3 h over silica gel at 23 °C. The effect of different treatments was evaluated by monitoring the regrowth of both non-frozen and cryo-preserved explants. After cryo-preservation, five genotypes achieved regrowth rates over 40% in at least one of the applied protocols, while two genotypes showed regrowth rates of around 10%. A significant improvement in regrowth success for all genotypes using both cryo-plate methods was achieved by pre-culturing shoot tips for 7 days on a medium containing 0.5 M sucrose in complete darkness at 4 °C. Shoots regenerated from cryo-preserved explants were further monitored in vitro. By the third subculture, they had not only regained but had even exceeded the multiplication capacity (index of multiplication, length of axial, and lateral shoots) of shoots regenerated from dissection controls. Following multiplication, the cryo-preserved shoots were successfully rooted and rooting ability was assessed by monitoring the percentage of rooting, number and length of roots, and height of rooted plantlets.
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Claims 5
Claim · model-written summary
Five genotypes regrew at rates above 40% under at least one cryopreservation protocol.
- Taxon
- Plums
Prunus domesticat0 - Outcome
- regrowth ratese0
- Value
- over 40%e0
- Qualifier
- five genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Plums”
e0“After cryo-preservation, five genotypes achieved regrowth rates over 40% in at least one of the applied protocols”
Claim · model-written summary
Two genotypes regrew at about 10% after cryopreservation.
- Taxon
- PlumsPrunus domesticat0
- Outcome
- regrowth ratese0
- Value
- around 10%e0
- Qualifier
- two genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Plums”
e0“two genotypes showed regrowth rates of around 10%”
Claim · model-written summary
Seven-day preculture on sucrose at 4 °C improved regrowth for all genotypes with both cryoplate methods.
- Taxon
- PlumsPrunus domesticat0
- Treatment
- pre-culturing shoot tips for 7 days on a medium containing 0.5 M sucrose in complete darkness at 4 °Ce0
- Outcome
- regrowth successe0
- Direction
- improvemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Plums”
e0“A significant improvement in regrowth success for all genotypes using both cryo-plate methods was achieved by pre-culturing shoot tips for 7 days on a medium containing 0.5 M sucrose in complete darkness at 4 °C”
Claim · model-written summary
By the third subculture, shoots from cryopreserved specimens exceeded controls in multiplication index and axial and lateral shoot length.
- Taxon
- PlumsPrunus domesticat0
- Treatment
- cryo-preservede1
- Outcome
- multiplication capacity (index of multiplication, length of axial, and lateral shoots)e0
- Direction
- exceedede0
- Comparator
- shoots regenerated from dissection controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Plums”
e0“By the third subculture, they had not only regained but had even exceeded the multiplication capacity (index of multiplication, length of axial, and lateral shoots) of shoots regenerated from dissection controls”
e1“Following multiplication, the cryo-preserved shoots were successfully rooted”
Claim · model-written summary
Shoots from cryopreserved specimens rooted successfully after multiplication.
- Taxon
- PlumsPrunus domesticat0
- Organ / stage
- shootse0
- Outcome
- successfully rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Plums”
e0“Following multiplication, the cryo-preserved shoots were successfully rooted”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.