Abstract
Endophytic and seed-borne microbial attacks represent major barriers leading to poor regeneration- powered micro-propagation of Cannabis sativa. Thus, a reliable sterile method during germination and in vitro plantlet preparation is important for the further successful micro-propagation of C. sativa plants. In the present study, we aim to develop a quick and efficient procedure for the preparation of pathogen-free plantlets using a hydrogen peroxide (H2O2) solution as a liquid germination medium. In this framework, all three phases, i.e., seed sterilization, germination, and early seedling development, were carried out in H2O2 solutions of varying concentrations (0, 1, 3, 5, and 10%). Among the different concentrations tested, the 1% H2O2 solution resulted in the fastest and the most successful germination for all tested genotypes of C. sativa seeds, in this case “Cheungsam,” “Cherry Blossom,” “Queen Dream,” and “Hot Blonde.” Higher concentrations of H2O2 (3, 5, and 10%) adversely affected the germination rate of C. sativa seeds compared to 0% or 1% H2O2. In addition, 5% and 10% H2O2 solutions significantly reduced seedling survival rates on MS media. Importantly, contamination rates of 88.4–96.7% were observed for C. sativa seedlings germinated with the 0% H2O2 solution and transferred to antibiotic-free MS media. However, a contamination rate of less than 4.0% was observed for seedlings prepared using 1% H2O2 as a germination medium. Moreover, the survival rates of seedlings germinated in 1% H2O2 were significantly higher than those of other seedlings. Our results suggest that a 1% H2O2 solution is a useful liquid germination medium for C. sativa seeds to enhance germination and survival rates while decreasing microbial contamination in antibiotic-free tissue culture media.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
A 1% H2O2 medium gave the fastest germination across four Cannabis genotypes.
- Taxon
- C. sativa
Cannabis sativae0 - Material
- “Cheungsam,” “Cherry Blossom,” “Queen Dream,” and “Hot Blonde.”e0
- Treatment
- 1% H2O2 solutione0
- Outcome
- germinatione0
- Direction
- fasteste0
- Comparator
- different concentrations testede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the different concentrations tested, the 1% H2O2 solution resulted in the fastest and the most successful germination for all tested genotypes of C. sativa seeds, in this case “Cheungsam,” “Cherry Blossom,” “Queen Dream,” and “Hot Blonde.””
Claim · model-written summary
A 1% H2O2 medium gave the most successful germination across four Cannabis genotypes.
- Taxon
- C. sativaCannabis sativae0
- Treatment
- 1% H2O2 solutione0
- Outcome
- germinatione0
- Direction
- most successfule0
- Comparator
- different concentrations testede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the different concentrations tested, the 1% H2O2 solution resulted in the fastest and the most successful germination for all tested genotypes of C. sativa seeds, in this case “Cheungsam,” “Cherry Blossom,” “Queen Dream,” and “Hot Blonde.””
Claim · model-written summary
Higher H2O2 concentrations reduced Cannabis seed germination relative to 0% or 1%.
- Taxon
- C. sativaCannabis sativae0
- Treatment
- Higher concentrations of H2O2 (3, 5, and 10%)e0
- Outcome
- germination ratee0
- Direction
- adversely affectede0
- Comparator
- 0% or 1% H2O2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher concentrations of H2O2 (3, 5, and 10%) adversely affected the germination rate of C. sativa seeds compared to 0% or 1% H2O2.”
Claim · model-written summary
5% H2O2 reduced seedling survival on MS media.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- 5%e0
- Outcome
- seedling survival ratese0
- Direction
- reducede0
- Setting
- MS mediae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5% and 10% H2O2 solutions significantly reduced seedling survival rates on MS media.”
t0“Cannabis sativa”
Claim · model-written summary
10% H2O2 reduced seedling survival on MS media.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- 10%e0
- Outcome
- seedling survival ratese0
- Direction
- reducede0
- Setting
- MS mediae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5% and 10% H2O2 solutions significantly reduced seedling survival rates on MS media.”
t0“Cannabis sativa”
Claim · model-written summary
Seedlings germinated without H2O2 had 88.4–96.7% contamination after transfer to antibiotic-free MS medium.
- Taxon
- C. sativaCannabis sativae0
- Treatment
- 0% H2O2 solutione0
- Outcome
- contamination ratese0
- Value
- 88.4–96.7%e0
- Setting
- antibiotic-free MS mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“contamination rates of 88.4–96.7% were observed for C. sativa seedlings germinated with the 0% H2O2 solution and transferred to antibiotic-free MS media.”
Claim · model-written summary
Seedlings germinated in 1% H2O2 had less than 4.0% contamination.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- 1% H2O2e0
- Outcome
- contamination ratee0
- Value
- less than 4.0%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a contamination rate of less than 4.0% was observed for seedlings prepared using 1% H2O2 as a germination medium.”
t0“Cannabis sativa”
Claim · model-written summary
Seedlings germinated in 1% H2O2 survived at higher rates than other seedlings.
- Taxon
- Cannabis sativaCannabis sativat0
- Treatment
- 1% H2O2e0
- Outcome
- survival ratese0
- Direction
- highere0
- Comparator
- other seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the survival rates of seedlings germinated in 1% H2O2 were significantly higher than those of other seedlings.”
t0“Cannabis sativa”
Claim · model-written summary
The authors recommend 1% H2O2 as a liquid germination medium for Cannabis tissue culture.
- Taxon
- C. sativaCannabis sativae0
- Treatment
- 1% H2O2 solutione0
- Outcome
- useful liquid germination mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 1% H2O2 solution is a useful liquid germination medium for C. sativa seeds to enhance germination and survival rates while decreasing microbial contamination in antibiotic-free tissue culture media.”
The same questions in other papers
hydrogen peroxide · growth · Cannabis sativaResults agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.