Abstract
In higher plants, WRKY transcription factors are broadly involved in a variety of life activities and play an important role in both biotic and abiotic stress responses. However, little is known about the functions of WRKY genes in the popular species, such as Cucurbita maxima (pumpkin), which is planted worldwide. In the present study, 102 CmWRKY genes were identified in the C. maxima genome. Chromosome location, multiple sequence alignment, phylogenetic analysis, and synteny analysis of the CmWRKYs were performed. Notably, we found that silencing CmWRKY22 promoted cucumber mosaic virus (CMV) infection, whereas overexpression of CmWRKY22 inhibited the CMV infection. Subsequently, an electrophoretic mobility shift assay (EMSA) confirmed that CmWRKY22 was able to bind to the W-box at the promoter of CmPR1b, which is a responsive gene of the salicylic acid (SA) signaling pathway. In summary, this study has provided a foundation for the antiviral functions of WRKY transcription factors in C. maxima.
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Claims 4
Claim · model-written summary
The C. maxima genome contained 102 identified CmWRKY genes.
- Taxon
- C. maxima
Cucurbita maximae0 - Gene
- CmWRKYe0
- Outcome
- 102 CmWRKY genese0
- Value
- 102e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“102 CmWRKY genes were identified in the C. maxima genome”
Claim · model-written summary
Silencing CmWRKY22 promoted CMV infection in C. maxima.
- Taxon
- Cucurbita maximaCucurbita maximat0
- Treatment
- silencing CmWRKY22e0
- Interactor
- cucumber mosaic virus (CMV)
cucumber mosaic cucumoviruse0pathogen - Gene
- CmWRKY22e0
- Outcome
- cucumber mosaic virus (CMV) infectione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing CmWRKY22 promoted cucumber mosaic virus (CMV) infection”
t0“Cucurbita maxima”
Claim · model-written summary
Overexpression of CmWRKY22 inhibited CMV infection in C. maxima.
- Taxon
- Cucurbita maximaCucurbita maximat0
- Treatment
- overexpression of CmWRKY22e0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Gene
- CmWRKY22e0
- Outcome
- CMV infectione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of CmWRKY22 inhibited the CMV infection”
t0“Cucurbita maxima”
Claim · model-written summary
CmWRKY22 bound the W-box at the CmPR1b promoter.
- Taxon
- Cucurbita maximaCucurbita maximat0
- Gene
- CmWRKY22e0
- Outcome
- bind to the W-box at the promoter of CmPR1be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CmWRKY22 was able to bind to the W-box at the promoter of CmPR1b”
t0“Cucurbita maxima”