Abstract
Abstract Cold stress (CS) impact on crops is one of the critical constraints for sustainable and smart agricultural production. CS adversely affects plants leading to growth retardation, necrosis, chlorosis, and significant yield loss. The objective of this study was to explore the mechanistic basis of silicon (Si) in enhancing CS tolerance in alfalfa plants. The fluorescence staining indicated that Si-reduced the intensity of CS-induced superoxide radical (O 2 •– ) and hydrogen peroxide (H 2 O 2 ) generation in plants that improved plant photosynthesis, cellular integrity, and alfalfa biomass production under CS. The exogenous supplementation of Si significantly restored the endogenous Si status accompanied by the upregulation of NIP (nodulin 26-like intrinsic protein) genes NIP2 , NIP5;1 , and NIP6;1 in alfalfa. The elemental concentration analysis revealed that exogenous silicon (E-Si) triggers the increase of calcium (Ca), magnesium (Mg), and sulfur (S) in plants subjected to Si-supplementation compared to the plants cultivated without Si under CS. The application of Si significantly increased the activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR). Furthermore, Si significantly enhanced the expression of CS-responsive candidate genes including ICE1 , CBF1/DREB1C , CBF2/DREB1B , CBF3/DREB1A , COR15A , COR47 , and KIN1 in alfalfa. These findings together provide mechanistic insights into Si-involving CS tolerance in alfalfa. This eco-friendly SC management strategy using Si treatment can be useful to plant breeders and farmers for developing CS-resilient smart alfalfa production through breeding program.
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Claims 8
Claim · model-written summary
Silicon reduced cold-induced superoxide and hydrogen peroxide generation.
- Taxon
- alfalfa
Medicago sativat0 - Treatment
- Sie0
- Outcome
- superoxide radical (O 2 •– ) and hydrogen peroxide (H 2 O 2 ) generatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Si-reduced the intensity of CS-induced superoxide radical (O 2 •– ) and hydrogen peroxide (H 2 O 2 ) generation in plants”
t0“alfalfa”
Claim · model-written summary
Silicon improved alfalfa photosynthesis, cellular integrity and biomass under cold stress.
- Taxon
- alfalfaMedicago sativae0
- Outcome
- plant photosynthesis, cellular integrity, and alfalfa biomass productione0
- Direction
- improvede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“improved plant photosynthesis, cellular integrity, and alfalfa biomass production under CS”
Claim · model-written summary
Exogenous silicon significantly restored endogenous silicon status.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- exogenous supplementation of Sie0
- Outcome
- endogenous Si statuse0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous supplementation of Si significantly restored the endogenous Si status”
t0“alfalfa”
Claim · model-written summary
Silicon upregulated NIP2, NIP5;1 and NIP6;1 genes.
- Taxon
- alfalfaMedicago sativae0
- Gene
- NIP2 , NIP5;1 , and NIP6;1e0
- Outcome
- upregulation of NIP (nodulin 26-like intrinsic protein) genes NIP2 , NIP5;1 , and NIP6;1e0
- Direction
- upregulatione0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“upregulation of NIP (nodulin 26-like intrinsic protein) genes NIP2 , NIP5;1 , and NIP6;1 in alfalfa”
Claim · model-written summary
Exogenous silicon increased calcium, magnesium and sulfur concentrations under cold stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Si-supplementatione0
- Outcome
- calcium (Ca), magnesium (Mg), and sulfur (S)e0
- Direction
- increasee0
- Comparator
- plants cultivated without Si under CSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous silicon (E-Si) triggers the increase of calcium (Ca), magnesium (Mg), and sulfur (S) in plants subjected to Si-supplementation compared to the plants cultivated without Si under CS”
t0“alfalfa”
Claim · model-written summary
Silicon significantly increased SOD, CAT, APX and GR activity.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Sie0
- Outcome
- activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Si significantly increased the activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR)”
t0“alfalfa”
Claim · model-written summary
Silicon significantly enhanced expression of cold-responsive candidate genes.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- Sie0
- Gene
- ICE1 , CBF1/DREB1C , CBF2/DREB1B , CBF3/DREB1A , COR15A , COR47 , and KIN1e0
- Outcome
- expression of CS-responsive candidate genese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Si significantly enhanced the expression of CS-responsive candidate genes including ICE1 , CBF1/DREB1C , CBF2/DREB1B , CBF3/DREB1A , COR15A , COR47 , and KIN1 in alfalfa”
Claim · model-written summary
The authors propose silicon treatment for cold-resilient alfalfa production.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- Si treatmente0
- Outcome
- developing CS-resilient smart alfalfa productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using Si treatment can be useful to plant breeders and farmers for developing CS-resilient smart alfalfa production”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.