Abstract
The potential antifungal activity of seed storage glycoprotein (7S globulin) isolated from Cowpea and Lupin seeds against the pathogenic fungus Pythium sp. that cause fruit rot in cucumber was tested in vitroandin vivoon cucumber fruitsafter the harvesting compared to control. The 7S globulin obtained from Cowpea had a high content of total amino acid (78.2%) against 77.27% for 7S globulin obtained from Lupin. Both protein fractions have similar electrophoretic patterns despite different band locations on the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The pathogen was identified in the Plant Pathology Department Lab., Faculty of Agriculture, Zagazig University, as Pythium sp. The mycelial growth of Pythium sp. in liquid media was evidently reduced in the presence of 7S globulin from Cowpea and Lupin in a concentration-dependent manner. The application of 500 µg/mL 7S globulin entirely destabilized and deformed this shape in both compounds (Lupin seed 7S and Cowpea seed 7S). Scanning electron microscopy (SEM) images of the mycelium of Pythium. sp. in inhibition zones induced by either Lupin 7S or Cowpea 7S revealed significant morphological changes in the hyphae, including deformation and contraction. The in vivo tests showed that both Cowpea 7S globulin and Lupine 7S globulin effectively reduced the incidence of postharvest Pythium. sp.infection on cucumber fruits. It can be concluded that there are indeed alternatives to replace the synthetic fungicides for the management of this notorious soil as well as seed-borne fungi (Pythium), which causes big agricultural losses.
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Claims 11
Claim · model-written summary
Cowpea 7S globulin had 78.2% total amino acid content.
- Taxon
- Cowpea
Vigna unguiculatae0 - Outcome
- total amino acide0
- Value
- 78.2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 7S globulin obtained from Cowpea had a high content of total amino acid (78.2%) against 77.27% for 7S globulin obtained from Lupin.”
Claim · model-written summary
Lupin 7S globulin had 77.27% total amino acid content.
LupinusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 7S globulin obtained from Cowpea had a high content of total amino acid (78.2%) against 77.27% for 7S globulin obtained from Lupin.”
Claim · model-written summary
The two seed protein fractions had similar electrophoretic patterns with different band locations.
Verbatim
e0“Both protein fractions have similar electrophoretic patterns despite different band locations on the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE).”
t0“LEGUME”
Claim · model-written summary
Cowpea 7S globulin reduced Pythium mycelial growth in a concentration-dependent manner.
- Taxon
- cucumber
Cucumis sativuse1 - Treatment
- 7S globulin from Cowpeae0
- Outcome
- mycelial growth of Pythium sp.e0
- Direction
- reducede0
.jpg?width=60){kind=small}
PythiumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The mycelial growth of Pythium sp. in liquid media was evidently reduced in the presence of 7S globulin from Cowpea and Lupin in a concentration-dependent manner.”
e1“fruit rot in cucumber”
Claim · model-written summary
Lupin 7S globulin reduced Pythium mycelial growth in a concentration-dependent manner.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- 7S globulin from Cowpea and Lupine0
- Outcome
- mycelial growth of Pythium sp.e0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mycelial growth of Pythium sp. in liquid media was evidently reduced in the presence of 7S globulin from Cowpea and Lupin in a concentration-dependent manner.”
e1“fruit rot in cucumber”
Claim · model-written summary
Lupin 7S globulin at 500 µg/mL deformed Pythium mycelium.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- 500 µg/mL 7S globuline0
- Interactor
- Pythium. sp.e2pathogen
- Outcome
- mycelium of Pythium. sp.e2
- Direction
- deformede0
- Qualifier
- Lupin seed 7Se0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The application of 500 µg/mL 7S globulin entirely destabilized and deformed this shape in both compounds (Lupin seed 7S and Cowpea seed 7S).”
e1“fruit rot in cucumber”
e2“mycelium of Pythium. sp.”
Claim · model-written summary
Cowpea 7S globulin at 500 µg/mL deformed Pythium mycelium.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- 500 µg/mL 7S globuline0
- Interactor
- Pythium. sp.e2pathogen
- Outcome
- mycelium of Pythium. sp.e2
- Direction
- deformede0
- Qualifier
- Cowpea seed 7Se0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The application of 500 µg/mL 7S globulin entirely destabilized and deformed this shape in both compounds (Lupin seed 7S and Cowpea seed 7S).”
e1“fruit rot in cucumber”
e2“mycelium of Pythium. sp.”
Claim · model-written summary
Cowpea 7S induced significant deformation and contraction of Pythium hyphae.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- Cowpea 7Se0
- Interactor
- Pythium. sp.e0pathogen
- Outcome
- morphological changes in the hyphae, including deformation and contractione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Scanning electron microscopy (SEM) images of the mycelium of Pythium. sp. in inhibition zones induced by either Lupin 7S or Cowpea 7S revealed significant morphological changes in the hyphae, including deformation and contraction.”
e1“fruit rot in cucumber”
Claim · model-written summary
Lupin 7S induced significant deformation and contraction of Pythium hyphae.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- Lupin 7Se0
- Interactor
- Pythium. sp.e0pathogen
- Outcome
- morphological changes in the hyphae, including deformation and contractione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Scanning electron microscopy (SEM) images of the mycelium of Pythium. sp. in inhibition zones induced by either Lupin 7S or Cowpea 7S revealed significant morphological changes in the hyphae, including deformation and contraction.”
e1“fruit rot in cucumber”
Claim · model-written summary
Cowpea 7S globulin reduced postharvest Pythium infection incidence on cucumber fruit.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- fruitse0
- Treatment
- Cowpea 7S globuline0
- Interactor
- Pythium. sp.e0pathogen
- Outcome
- incidence of postharvest Pythium. sp.infectione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both Cowpea 7S globulin and Lupine 7S globulin effectively reduced the incidence of postharvest Pythium. sp.infection on cucumber fruits”
Claim · model-written summary
Lupine 7S globulin reduced postharvest Pythium infection incidence on cucumber fruit.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- fruitse0
- Treatment
- Lupine 7S globuline0
- Interactor
- Pythium. sp.e0pathogen
- Outcome
- incidence of postharvest Pythium. sp.infectione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both Cowpea 7S globulin and Lupine 7S globulin effectively reduced the incidence of postharvest Pythium. sp.infection on cucumber fruits”
The same questions in other papers
Vigna unguiculata · disease · Cucumis sativus (cucumber)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.