Paper · 2023 · Agronomy

Phenology, Nitrogen Status, and Yield of Red Clover (Trifolium pretense L.) Affected by Application of Vitamin B12, Humic Acid, and Enriched Biochar

Dorna Saadat; Arthur Siller; Masoud Hashemi

10
claims
33
verbatim quotes
6
citations
3
research areas
✓
Open access · cc-by

Abstract

Studies on vitamin B12’s influence on the flowering and yield parameters of red clover (Trifolium pretense L.) are not well documented. A greenhouse experiment investigated the effect of the solo and combined application of vitamin B12 with humic acid and enriched biochar on the morphology, nitrogen status, and biomass yield of the shoots and roots of red clovers. Two levels of vitamin B12 (0, 20 mg pot−1) mixed with four growing media were laid out as a randomized complete block design. The results indicated that vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days), while the co-application of B12 and humic acid resulted in further reduction (84.5 days); (B) an increased total number of stems (73.0 to 78.6 plant−1); (C) a boost in the root dry weight by 60% (3.8 to 6.4 g) while having no significant influence on the shoot dry weight; (D) a decrease in the leaf trichome density by 30% (49.0 to 35.0 plant−1); (E) a rise in the N content of the roots (107.8 to 173.3 mg plant−1), while having no influence on shoot N content. biochar’s influence on the phenology and productivity of red clovers was minimal. The results highlighted the importance of the application of manure, which is naturally rich in vitamin B12 and humic acid, to forage crops, including red clover.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 10

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Vitamin B12 decreased red clover flowering time by 5 days (100.2 to 95.9 days)

Taxon
red cloverTrifolium pratensee0
Treatment
vitamin B12e1
Outcome
flowering timee1
Direction
decreasee1
Value
5 dayse1
Comparator
0e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e7

“Two levels of vitamin B12 (0, 20 mg pot−1)”

✓ located verbatim in the source · abstract, characters 367–409 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Co-application of vitamin B12 and humic acid further reduced flowering time to 84.5 days

Taxon
red cloverTrifolium pratensee0
Treatment
co-application of B12 and humic acide2
Outcome
flowering timee1
Direction
reductione2
Value
84.5 dayse2
Comparator
vitamin B12e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e2

“the co-application of B12 and humic acid resulted in further reduction (84.5 days)”

✓ located verbatim in the source · abstract, characters 623–705 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Vitamin B12 increased the total number of red clover stems from 73.0 to 78.6 per plant

Taxon
red cloverTrifolium pratensee0
Treatment
vitamin B12e1
Outcome
total number of stemse3
Direction
increasede3
Value
73.0 to 78.6 plant−1e3
Comparator
0e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e3

“(B) an increased total number of stems (73.0 to 78.6 plant−1)”

✓ located verbatim in the source · abstract, characters 707–768 · show in text ↑
e7

“Two levels of vitamin B12 (0, 20 mg pot−1)”

✓ located verbatim in the source · abstract, characters 367–409 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Vitamin B12 boosted red clover root dry weight by 60% (3.8 to 6.4 g)

Taxon
red cloverTrifolium pratensee0
Treatment
vitamin B12e1
Outcome
root dry weighte4
Direction
booste4
Value
60%e4
Qualifier
3.8 to 6.4 ge4
Comparator
0e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e4

“a boost in the root dry weight by 60% (3.8 to 6.4 g) while having no significant influence on the shoot dry weight”

✓ located verbatim in the source · abstract, characters 774–888 · show in text ↑
e7

“Two levels of vitamin B12 (0, 20 mg pot−1)”

✓ located verbatim in the source · abstract, characters 367–409 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Vitamin B12 had no significant influence on red clover shoot dry weight

Taxon
red cloverTrifolium pratensee0
Treatment
vitamin B12e1
Outcome
shoot dry weighte4
Direction
no significant influencee4
Comparator
0e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e4

“a boost in the root dry weight by 60% (3.8 to 6.4 g) while having no significant influence on the shoot dry weight”

✓ located verbatim in the source · abstract, characters 774–888 · show in text ↑
e7

“Two levels of vitamin B12 (0, 20 mg pot−1)”

✓ located verbatim in the source · abstract, characters 367–409 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Vitamin B12 decreased leaf trichome density of red clover by 30% (49.0 to 35.0 plant−1)

Taxon
red cloverTrifolium pratensee0
Treatment
vitamin B12e1
Outcome
leaf trichome densitye5
Direction
decreasee5
Value
30%e5
Qualifier
49.0 to 35.0 plant−1e5
Comparator
0e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e5

“(D) a decrease in the leaf trichome density by 30% (49.0 to 35.0 plant−1)”

✓ located verbatim in the source · abstract, characters 890–963 · show in text ↑
e7

“Two levels of vitamin B12 (0, 20 mg pot−1)”

✓ located verbatim in the source · abstract, characters 367–409 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Vitamin B12 raised N content of red clover roots (107.8 to 173.3 mg plant−1)

Taxon
red cloverTrifolium pratensee0
Treatment
vitamin B12e1
Outcome
N content of the rootse6
Direction
risee6
Value
107.8 to 173.3 mg plant−1e6
Comparator
0e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e6

“a rise in the N content of the roots (107.8 to 173.3 mg plant−1), while having no influence on shoot N content”

✓ located verbatim in the source · abstract, characters 969–1079 · show in text ↑
e7

“Two levels of vitamin B12 (0, 20 mg pot−1)”

✓ located verbatim in the source · abstract, characters 367–409 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Vitamin B12 had no influence on red clover shoot N content

Taxon
red cloverTrifolium pratensee0
Treatment
vitamin B12e1
Outcome
shoot N contente6
Direction
no influencee6
Comparator
0e7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the flowering and yield parameters of red clover (Trifolium pretense L.)”

✓ located verbatim in the source · abstract, characters 38–110 · show in text ↑
e1

“vitamin B12 markedly led to (A) a decrease in the flowering time by 5 days (100.2 to 95.9 days)”

✓ located verbatim in the source · abstract, characters 520–615 · show in text ↑
e6

“a rise in the N content of the roots (107.8 to 173.3 mg plant−1), while having no influence on shoot N content”

✓ located verbatim in the source · abstract, characters 969–1079 · show in text ↑
e7

“Two levels of vitamin B12 (0, 20 mg pot−1)”

✓ located verbatim in the source · abstract, characters 367–409 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Enriched biochar had minimal influence on red clover phenology and productivity

Taxon
red cloversTrifolium pratensee7
Treatment
enriched biochart1
Outcome
phenology and productivitye7
Qualifier
minimale7

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t1

“Enriched Biochar”

✓ located verbatim in the source · title, characters 132–148 · show in text ↑
e7

“influence on the phenology and productivity of red clovers was minimal”

✓ located verbatim in the source · abstract, characters 1091–1161 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Manure, naturally rich in vitamin B12 and humic acid, is important to apply to forage crops including red clover

Taxon
red cloverTrifolium pratensee0
Outcome
the application of manuree0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Trifolium pratense
Trifolium pratense · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the importance of the application of manure, which is naturally rich in vitamin B12 and humic acid, to forage crops, including red clover”

✓ located verbatim in the source · abstract, characters 1187–1324 · show in text ↑