Abstract
The phloem-feeding insect Bemisia tabaci is an important pest, responsible for the transmission of several crop-threatening virus species. While feeding, the insect secretes a cocktail of effectors to modulate plant defense responses. Here, we present a set of proteins identified in an artificial diet on which B. tabaci was salivating. We subsequently studied whether these candidate effectors can play a role in plant immune suppression. Effector G4 was the most robust suppressor of an induced- reactive oxygen species (ROS) response in Nicotiana benthamiana. In addition, G4 was able to suppress ROS production in Solanum lycopersicum (tomato) and Capsicum annuum (pepper). G4 localized predominantly in the endoplasmic reticulum in N. benthamiana leaves and colocalized with two identified target proteins in tomato: REF-like stress related protein 1 (RSP1) and meloidogyne-induced giant cell protein DB141 (MIPDB141). Silencing of MIPDB141 in tomato reduced whitefly fecundity up to 40%, demonstrating that the protein is involved in susceptibility to B. tabaci. Together, our data demonstrate that effector G4 impairs tomato immunity to whiteflies by interfering with ROS production and via an interaction with tomato susceptibility protein MIPDB141. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Effector G4 was the most robust suppressor of the induced ROS response in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- Effector G4e1
- Outcome
- induced- reactive oxygen species (ROS) responsee1
- Direction
- suppressore1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e1“Effector G4 was the most robust suppressor of an induced- reactive oxygen species (ROS) response in Nicotiana benthamiana”
Claim · model-written summary
Effector G4 suppressed ROS production in tomato.
- Taxon
- Solanum lycopersicum (tomato)
Solanum lycopersicume2 - Treatment
- G4e2
- Outcome
- ROS productione2
- Direction
- suppresse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e2“G4 was able to suppress ROS production in Solanum lycopersicum (tomato) and Capsicum annuum (pepper)”
Claim · model-written summary
Effector G4 suppressed ROS production in pepper.
- Taxon
- Capsicum annuum (pepper)
Capsicum annuume2 - Treatment
- G4e2
- Outcome
- ROS productione2
- Direction
- suppresse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e2“G4 was able to suppress ROS production in Solanum lycopersicum (tomato) and Capsicum annuum (pepper)”
Claim · model-written summary
G4 localized predominantly in the endoplasmic reticulum in Nicotiana benthamiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae3
- Organ / stage
- leavese3
- Treatment
- G4e3
- Outcome
- localized predominantly in the endoplasmic reticulume3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e3“G4 localized predominantly in the endoplasmic reticulum in N. benthamiana leaves and colocalized with two identified target proteins in tomato”
Claim · model-written summary
G4 colocalized with the tomato proteins RSP1 and MIPDB141.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- G4e3
- Gene
- REF-like stress related protein 1 (RSP1) and meloidogyne-induced giant cell protein DB141 (MIPDB141)e3
- Outcome
- colocalizede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e3“G4 localized predominantly in the endoplasmic reticulum in N. benthamiana leaves and colocalized with two identified target proteins in tomato: REF-like stress related protein 1 (RSP1) and meloidogyne-induced giant cell protein DB141 (MIPDB141)”
Claim · model-written summary
Silencing MIPDB141 in tomato reduced whitefly fecundity by up to 40%.
- Taxon
- tomatoSolanum lycopersicume4
- Treatment
- Silencing of MIPDB141e4
- Interactor
- whiteflye4pest
- Gene
- MIPDB141e4
- Outcome
- whitefly fecunditye4
- Direction
- reducede4
- Value
- 40%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e4“Silencing of MIPDB141 in tomato reduced whitefly fecundity up to 40%, demonstrating that the protein is involved in susceptibility to B. tabaci”
Claim · model-written summary
MIPDB141 is involved in tomato susceptibility to Bemisia tabaci.
- Taxon
- tomatoSolanum lycopersicume4
- Interactor
- B. tabaci
Bemisia tabacie4pest - Gene
- MIPDB141e4
- Outcome
- susceptibility to B. tabacie4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e4“Silencing of MIPDB141 in tomato reduced whitefly fecundity up to 40%, demonstrating that the protein is involved in susceptibility to B. tabaci”
Claim · model-written summary
The authors identified a set of candidate salivary proteins of B. tabaci secreted into an artificial diet.
- Taxon
- TomatoSolanum lycopersicume0
- Interactor
- B. tabaciBemisia tabacie5pest
- Outcome
- a set of proteins identified in an artificial diete5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Interaction of Whitefly Effector G4 with Tomato Proteins Impacts Whitefly Performance”
e5“Here, we present a set of proteins identified in an artificial diet on which B. tabaci was salivating”
The same questions in other papers
pathogen product · physiology: oxidative · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
loss of function · pest · Solanum lycopersicum (tomato)Positive and negative results
6 papers in the same direction as this paper · 10 in the opposite direction
- no effect
- negative
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.