Abstract
Peanut root rot, caused by Fusarium spp., is a devastating fungal disease. As part of a program to obtain a biocontrol agent to control peanut root rot in the field, a bacterial strain LY-1 capable of inhibiting the growth of the fungus in vitro was isolated from rhizosphere soil samples collected from wild mint by agar disk dilution and dual-culture assay. Strain LY-1 was identified as Bacillus subtilis based on morphological characteristics, 16S rDNA, and gyrA sequence analyses. The bacterial suspension and cell-free culture filtrate of LY-1 could significantly inhibit the growth of Fusarium oxysporum, Fusarium proliferatum and Fusarium solani, but volatile organic compounds from the cultures had only a weak effect on mycelial growth. The percentage inhibition of 20% concentration of the cell-free culture filtrate of LY-1 on conidium production of each of the three Fusarium species was greater than 72.38%, and the percentage inhibition by the culture filtration on the germination of conidia of the three species was at least 62.37%. The production of extracellular enzyme activity by LY-1 was studied in functional assays, showing protease, cellulase, amylase, chitinase, and β-1,3-glucanase activity, while LY-1 contained a gene encoding iturin, an antifungal lipopeptide. In addition, under pot culture in a greenhouse, culture filtrate of LY-1 significantly promoted the growth of peanut, increasing the fresh and dry mass of the plant by 30.77% and 27.27%, respectively, in comparison with the no-filtrate control. The culture filtrate of LY-1 increased the resistance of peanut plants to F. oxysporum, with the biocontrol efficiency reaching 44.71%. In conclusion, B. subtilis LY-1, a plant-growth-promoting rhizobacterium, was able to protect peanuts from Fusarium spp. infection.
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Claims 10
Claim · model-written summary
LY-1 was isolated from wild mint rhizosphere soil and identified as Bacillus subtilis.
- Taxon
- wild mint
Mentha arvensise0 - Interactor
- LY-1e0biocontrol_agent
- Outcome
- a bacterial strain LY-1 capable of inhibiting the growth of the fungus in vitro was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a bacterial strain LY-1 capable of inhibiting the growth of the fungus in vitro was isolated from rhizosphere soil samples collected from wild mint by agar disk dilution and dual-culture assay.”
Claim · model-written summary
LY-1 suspension and filtrate significantly inhibited Fusarium oxysporum, F. proliferatum and F. solani growth.
- Taxon
- Peanut
Arachis hypogaeat0 - Treatment
- The bacterial suspension and cell-free culture filtrate of LY-1e0
- Interactor
- Fusarium oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume0pathogen - Second interactor
- LY-1e0biocontrol_agent
- Outcome
- growth of Fusarium oxysporum, Fusarium proliferatum and Fusarium solanie0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e0“The bacterial suspension and cell-free culture filtrate of LY-1 could significantly inhibit the growth of Fusarium oxysporum, Fusarium proliferatum and Fusarium solani, but volatile organic compounds from the cultures had only a weak effect on mycelial growth.”
t0“Peanut”
Claim · model-written summary
LY-1 volatiles had a weak inhibitory effect on fungal mycelial growth.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- volatile organic compounds from the culturese0
- Outcome
- mycelial growthe0
- Qualifier
- only a weak effecte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“volatile organic compounds from the cultures had only a weak effect on mycelial growth.”
t0“Peanut”
Claim · model-written summary
At 20% concentration, LY-1 filtrate inhibited conidium production by greater than 72.38%.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- 20% concentration of the cell-free culture filtrate of LY-1e0
- Second interactor
- LY-1e0biocontrol_agent
- Outcome
- conidium production of each of the three Fusarium speciese0
- Direction
- inhibitione0
- Value
- greater than 72.38%e0
FusariumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage inhibition of 20% concentration of the cell-free culture filtrate of LY-1 on conidium production of each of the three Fusarium species was greater than 72.38%”
t0“Peanut”
Claim · model-written summary
LY-1 filtrate inhibited Fusarium conidial germination by at least 62.37%.
- Taxon
- PeanutArachis hypogaeat0
- Treatment
- culture filtratione0
- Outcome
- germination of conidia of the three speciese0
- Direction
- inhibitione0
- Value
- at least 62.37%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the percentage inhibition by the culture filtration on the germination of conidia of the three species was at least 62.37%.”
t0“Peanut”
Claim · model-written summary
LY-1 displayed protease, cellulase, amylase, chitinase and β-1,3-glucanase activities.
- Taxon
- PeanutArachis hypogaeat0
- Outcome
- protease, cellulase, amylase, chitinase, and β-1,3-glucanase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“showing protease, cellulase, amylase, chitinase, and β-1,3-glucanase activity”
t0“Peanut”
Claim · model-written summary
LY-1 contained a gene encoding iturin.
- Taxon
- PeanutArachis hypogaeat0
- Gene
- a gene encoding iturine0
- Outcome
- contained a gene encoding iturine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LY-1 contained a gene encoding iturin, an antifungal lipopeptide.”
t0“Peanut”
Claim · model-written summary
LY-1 filtrate raised peanut fresh mass by 30.77% relative to no filtrate.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- culture filtrate of LY-1e0
- Interactor
- LY-1e0biocontrol_agent
- Outcome
- fresh and dry masse0
- Direction
- increasinge0
- Value
- 30.77%e0
- Comparator
- no-filtrate controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culture filtrate of LY-1 significantly promoted the growth of peanut, increasing the fresh and dry mass of the plant by 30.77% and 27.27%, respectively, in comparison with the no-filtrate control.”
Claim · model-written summary
LY-1 filtrate raised peanut dry mass by 27.27% relative to no filtrate.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- culture filtrate of LY-1e0
- Interactor
- LY-1e0biocontrol_agent
- Outcome
- dry masse0
- Direction
- increasinge0
- Value
- 27.27%e0
- Comparator
- no-filtrate controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culture filtrate of LY-1 significantly promoted the growth of peanut, increasing the fresh and dry mass of the plant by 30.77% and 27.27%, respectively, in comparison with the no-filtrate control.”
Claim · model-written summary
LY-1 filtrate increased peanut resistance to F. oxysporum, with 44.71% biocontrol efficiency.
- Taxon
- peanutArachis hypogaeae0
- Treatment
- culture filtrate of LY-1e0
- Interactor
- F. oxysporumFusarium oxysporume0pathogen
- Second interactor
- LY-1e0biocontrol_agent
- Outcome
- resistance of peanut plants to F. oxysporume0
- Direction
- increasede0
- Value
- 44.71%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The culture filtrate of LY-1 increased the resistance of peanut plants to F. oxysporum, with the biocontrol efficiency reaching 44.71%.”
The same questions in other papers
pathogen product · growth · Arachis hypogaea (peanut)Positive and negative results
4 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.