Abstract
Abstract Key message A new Or Anom1 gene introgressed in cultivated sunflower from wild Helianthus anomalus confers late post-attachment resistance to Orobanche cumana race G and maps to a target interval in Chromosome 4 where two receptor-like kinases (RLKs) have been identified in the H. anomalus genome as putative candidates. Abstract Sunflower broomrape is a parasitic weed that infects sunflower ( Helianthus annuus L.) roots causing severe yield losses. Breeding for resistance is the most effective and sustainable control method. In this study, we report the identification, introgression, and genetic and physiological characterization of a new sunflower source of resistance to race G of broomrape developed from the wild annual sunflower H. anomalus (accession PI 468642). Crosses between PI 468642 and the susceptible line P21 were carried out, and the genetic study was conducted in BC 1 F 1 , BC 1 F 2, and its derived BC 1 F 3 populations. A BC 1 F 5 germplasm named ANOM1 was developed through selection for race G resistance and resemblance to cultivated sunflower. The resistant trait showed monogenic and dominant inheritance. The gene, named Or Anom1 , was mapped to Chromosome 4 within a 1.2 cM interval and co-segregated with 7 SNP markers. This interval corresponds to a 1.32 Mb region in the sunflower reference genome, housing a cluster of receptor-like kinase and receptor-like protein (RLK-RLP) genes. Notably, the analysis of the H. anomalus genome revealed the absence of RLPs in the Or Anom1 target region but featured two RLKs as possible Or Anom1 candidates. Rhizotron and histological studies showed that Or Anom1 determines a late post-attachment resistance mechanism. Broomrape can establish a vascular connection with the host, but parasite growth is stopped before tubercle development, showing phenolic compounds accumulation and tubercle necrosis. ANOM1 will contribute to broadening the genetic basis of broomrape resistance in the cultivated sunflower pool and to a better understanding of the molecular basis of the sunflower-broomrape interaction.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
The new Or Anom1 introgression confers late post-attachment resistance to broomrape race G.
- Taxon
- sunflower
Helianthus annuuse0 - Material
- Helianthus anomaluse0
- Interactor
- Orobanche cumana
Orobanche cernua var. cumanae0parasite - Gene
- Or Anom1e0
- Outcome
- late post-attachment resistance to Orobanche cumana race Ge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A new Or Anom1 gene introgressed in cultivated sunflower from wild Helianthus anomalus confers late post-attachment resistance to Orobanche cumana race G”
Claim · model-written summary
Selection produced the ANOM1 BC1F5 germplasm for race G resistance and cultivated sunflower resemblance.
- Taxon
- sunflowerHelianthus annuuse0
- Material
- ANOM1e0
- Interactor
- Orobanche cumanaOrobanche cernua var. cumanae1parasite
- Outcome
- germplasm named ANOM1 was developede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A BC 1 F 5 germplasm named ANOM1 was developed through selection for race G resistance and resemblance to cultivated sunflower”
e1“Orobanche cumana race G”
Claim · model-written summary
The resistance trait showed monogenic dominant inheritance.
- Taxon
- sunflowerHelianthus annuuse1
- Interactor
- Orobanche cumanaOrobanche cernua var. cumanae2parasite
- Outcome
- monogenic and dominant inheritancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The resistant trait showed monogenic and dominant inheritance”
e1“sunflower source of resistance to race G of broomrape”
e2“Orobanche cumana race G”
Claim · model-written summary
Or Anom1 mapped to a 1.2 cM Chromosome 4 interval and co-segregated with seven SNP markers.
- Taxon
- sunflowerHelianthus annuuse1
- Gene
- Or Anom1e0
- Outcome
- mapped to Chromosome 4e0
- Value
- 1.2 cMe0
- Qualifier
- co-segregated with 7 SNP markerse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Or Anom1 , was mapped to Chromosome 4 within a 1.2 cM interval and co-segregated with 7 SNP markers”
e1“resemblance to cultivated sunflower”
Claim · model-written summary
The target interval corresponds to a 1.32 Mb sunflower reference region with RLK-RLP genes.
- Taxon
- sunflowerHelianthus annuuse0
- Gene
- receptor-like kinase and receptor-like protein (RLK-RLP) genese0
- Outcome
- region in the sunflower reference genomee0
- Value
- 1.32 Mbe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This interval corresponds to a 1.32 Mb region in the sunflower reference genome, housing a cluster of receptor-like kinase and receptor-like protein (RLK-RLP) genes”
Claim · model-written summary
The H. anomalus target region lacked RLPs.
- Taxon
- H. anomalus
Helianthus anomaluse0 - Gene
- RLPse0
- Outcome
- absence of RLPse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the H. anomalus genome revealed the absence of RLPs in the Or Anom1 target region”
Claim · model-written summary
Two RLKs in the H. anomalus region were proposed as Or Anom1 candidates.
- Taxon
- H. anomalusHelianthus anomaluse1
- Gene
- RLKse0
- Outcome
- possible Or Anom1 candidatese0
- Value
- two RLKse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“featured two RLKs as possible Or Anom1 candidates”
e1“analysis of the H. anomalus genome”
Claim · model-written summary
Broomrape established a vascular connection with the sunflower host.
- Taxon
- sunflowerHelianthus annuuse1
- Interactor
- Broomrapee0parasite
- Outcome
- establish a vascular connection with the hoste0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Broomrape can establish a vascular connection with the host”
e1“Sunflower broomrape is a parasitic weed that infects sunflower”
Claim · model-written summary
Broomrape growth stopped before tubercle development in resistant sunflower.
- Taxon
- sunflowerHelianthus annuuse1
- Interactor
- Broomrapee2parasite
- Outcome
- parasite growthe0
- Direction
- stoppede0
- Qualifier
- before tubercle developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“parasite growth is stopped before tubercle development”
e1“Sunflower broomrape is a parasitic weed that infects sunflower”
e2“Broomrape can establish”
Claim · model-written summary
Resistance involved phenolic compound accumulation and tubercle necrosis.
- Taxon
- sunflowerHelianthus annuuse1
- Interactor
- Broomrapee2parasite
- Outcome
- phenolic compounds accumulation and tubercle necrosise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“showing phenolic compounds accumulation and tubercle necrosis”
e1“Sunflower broomrape is a parasitic weed that infects sunflower”
e2“Broomrape can establish”