Abstract
Male sterility is a valuable trait for hybrid seed production in tomato (Solanum lycopersicum). The mutants male sterile-30 (ms-30) and ms-33 of tomato exhibit twisted stamens, exposed stigmas, and complete male sterility, thus holding potential for application in hybrid seed production. In this study, the ms-30 and ms-33 loci were fine-mapped to 53.3 kb and 111.2 kb intervals, respectively. Tomato PISTILLATA (TPI, syn. SlGLO2), a B-class MADS-box transcription factor gene, was identified as the most likely candidate gene for both loci. TPI is also the candidate gene of tomato male sterile mutant 7B-1 and sl-2. Allelism tests revealed that ms-30, ms-33, 7B-1, and sl-2 were allelic. Sequencing analysis showed sequence alterations in the TPI gene in all these mutants, with ms-30 exhibiting a transversion (G to T) that resulted in a missense mutation (S to I); ms-33 showing a transition (A to T) that led to alternative splicing, resulting in a loss of 46 amino acids in protein; and 7B-1 and sl-2 mutants showing the insertion of an approximately 4.8 kb retrotransposon. On the basis of these sequence alterations, a Kompetitive Allele Specific PCR marker, a sequencing marker, and an Insertion/Deletion marker were developed. Phenotypic analysis of the TPI gene-edited mutants and allelism tests indicated that the gene TPI is responsible for ms-30 and its alleles. Transcriptome analysis of ms-30 and quantitative RT-PCR revealed some differentially expressed genes associated with stamen and carpel development. These findings will aid in the marker-assisted selection for ms-30 and its alleles in tomato breeding and support the functional analysis of the TPI gene.
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Claims 11
Claim · model-written summary
ms-30 and ms-33 exhibit twisted stamens, exposed stigmas and complete male sterility.
- Taxon
- tomato
Solanum lycopersicume0 - Material
- male sterile-30 (ms-30) and ms-33e0
- Outcome
- twisted stamens, exposed stigmas, and complete male sterilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutants male sterile-30 (ms-30) and ms-33 of tomato exhibit twisted stamens, exposed stigmas, and complete male sterility”
Claim · model-written summary
ms-30 was fine mapped to 53.3 kb.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- ms-30e0
- Outcome
- fine-mappede0
- Value
- 53.3 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ms-30 and ms-33 loci were fine-mapped to 53.3 kb and 111.2 kb intervals, respectively”
t0“Tomato”
Claim · model-written summary
ms-33 was fine mapped to 111.2 kb.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- ms-33e0
- Outcome
- fine-mappede0
- Value
- 111.2 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ms-30 and ms-33 loci were fine-mapped to 53.3 kb and 111.2 kb intervals, respectively”
t0“Tomato”
Claim · model-written summary
TPI was identified as the likely gene for both loci and also for 7B-1 and sl-2.
- Taxon
- TomatoSolanum lycopersicume0
- Gene
- Tomato PISTILLATA (TPI, syn. SlGLO2)e0
- Outcome
- most likely candidate gene for both locie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato PISTILLATA (TPI, syn. SlGLO2), a B-class MADS-box transcription factor gene, was identified as the most likely candidate gene for both loci. TPI is also the candidate gene of tomato male sterile mutant 7B-1 and sl-2”
Claim · model-written summary
The four male sterile mutants were allelic.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- ms-30, ms-33, 7B-1, and sl-2e0
- Outcome
- were allelice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Allelism tests revealed that ms-30, ms-33, 7B-1, and sl-2 were allelic”
t0“Tomato”
Claim · model-written summary
ms-30 had a TPI G-to-T transversion producing an S-to-I missense change.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- ms-30e0
- Outcome
- a transversion (G to T) that resulted in a missense mutation (S to I)e0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ms-30 exhibiting a transversion (G to T) that resulted in a missense mutation (S to I)”
t0“Tomato”
Claim · model-written summary
ms-33 had a transition causing alternative splicing and loss of 46 amino acids.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- ms-33e0
- Outcome
- alternative splicinge0
- Value
- 46 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ms-33 showing a transition (A to T) that led to alternative splicing, resulting in a loss of 46 amino acids in protein”
t0“Tomato”
Claim · model-written summary
7B-1 and sl-2 had an approximately 4.8 kb retrotransposon insertion.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- 7B-1 and sl-2e0
- Outcome
- insertion of an approximately 4.8 kb retrotransposone0
- Value
- approximately 4.8 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7B-1 and sl-2 mutants showing the insertion of an approximately 4.8 kb retrotransposon”
t0“Tomato”
Claim · model-written summary
Three marker types were developed from the sequence alterations.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- a Kompetitive Allele Specific PCR marker, a sequencing marker, and an Insertion/Deletion marker were developede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On the basis of these sequence alterations, a Kompetitive Allele Specific PCR marker, a sequencing marker, and an Insertion/Deletion marker were developed”
t0“Tomato”
Claim · model-written summary
Gene editing and allelism tests indicated that TPI is responsible for ms-30 and its alleles.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- TPIe0
- Outcome
- responsible for ms-30 and its allelese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenotypic analysis of the TPI gene-edited mutants and allelism tests indicated that the gene TPI is responsible for ms-30 and its alleles”
t0“Tomato”
Claim · model-written summary
Transcriptome and RT-PCR analyses found genes associated with stamen and carpel development.
- Taxon
- TomatoSolanum lycopersicumt0
- Material
- ms-30e0
- Outcome
- some differentially expressed genes associated with stamen and carpel developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis of ms-30 and quantitative RT-PCR revealed some differentially expressed genes associated with stamen and carpel development”
t0“Tomato”