Abstract
Expansins (EXPs) loosen plant cell walls and are involved in diverse developmental processes through modifying cell-walls; however, little is known about the role of PpEXPs in peach fruit. In this study, 26 PpEXP genes were identified in the peach genome and grouped into four subfamilies, with 20 PpEXPAs, three PpEXPBs, one PpEXPLA and two PpEXPLBs. The 26 PpEXPs were mapped on eight chromosomes. The primary mode of gene duplication of the PpEXPs was dispersed gene duplication (DSD, 50%). Notably, cis-elements involved in light responsiveness and MeJA-responsiveness were detected in the promoter regions of all PpEXPs, while ethylene responsive elements were observed in 12 PpEXPs. Transcript profiling of PpEXPs in the peach fruit varieties of MF (melting), NMF (non-melting) and SH (stony hard) at different stages showed that PpEXPs displayed distinct expression patterns. Among the 26 PpEXPs, 15 PpEXPs were expressed in the fruit. Combining the expressing patterns of PpEXPs in fruits with different flesh textures, PpEXPA7, PpEXPA13 and PpEXPA15 were selected as candidate genes, as they were highly consistent with the patterns of previous reported key genes (PpPGM, PpPGF and PpYUC11) in regard to peach fruit texture. The genes with different expression patterns between MF and NMF were divided into 16 modules, of which one module, with pink and midnightblue, negatively correlated with the phenotype of fruit firmness and was identified as PpEXPA1 and PpEXPA7, while the other module was identified as PpERF in the pink module, which might potentially effect fruit texture development by regulating PpEXPs. These results provide a foundation for the functional characterization of PpEXPs in peach.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
A total of 26 PpEXP genes were identified in the peach genome and grouped into four subfamilies.
- Taxon
- peach
Prunus persicae7 - Gene
- PpEXP genese0
- Outcome
- identifiede0
- Value
- 26e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“26 PpEXP genes were identified in the peach genome and grouped into four subfamilies”
e7“peach genome”
Claim · model-written summary
The peach PpEXP family includes 20 PpEXPAs.
- Taxon
- peachPrunus persicae7
- Outcome
- PpEXPAse0
- Value
- 20e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“20 PpEXPAs”
e7“peach genome”
Claim · model-written summary
The peach PpEXP family includes three PpEXPBs.
- Taxon
- peachPrunus persicae7
- Outcome
- PpEXPBse0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three PpEXPBs”
e7“peach genome”
Claim · model-written summary
The peach PpEXP family includes one PpEXPLA.
- Taxon
- peachPrunus persicae7
- Outcome
- PpEXPLAe0
- Value
- onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one PpEXPLA”
e7“peach genome”
Claim · model-written summary
The peach PpEXP family includes two PpEXPLBs.
- Taxon
- peachPrunus persicae7
- Outcome
- PpEXPLBse0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two PpEXPLBs”
e7“peach genome”
Claim · model-written summary
The 26 PpEXPs were mapped on eight chromosomes.
- Taxon
- peachPrunus persicae7
- Outcome
- mapped on eight chromosomese0
- Value
- eight chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 26 PpEXPs were mapped on eight chromosomes”
e7“peach genome”
Claim · model-written summary
The primary mode of PpEXP gene duplication was dispersed gene duplication (50%).
- Taxon
- peachPrunus persicae7
- Outcome
- primary mode of gene duplicatione0
- Value
- 50%e0
- Qualifier
- dispersed gene duplicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The primary mode of gene duplication of the PpEXPs was dispersed gene duplication (DSD, 50%)”
e7“peach genome”
Claim · model-written summary
Light-responsive and MeJA-responsive cis-elements were detected in the promoters of all PpEXPs.
- Taxon
- peachPrunus persicae7
- Outcome
- cis-elements involved in light responsiveness and MeJA-responsivenesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cis-elements involved in light responsiveness and MeJA-responsiveness were detected in the promoter regions of all PpEXPs”
e7“peach genome”
Claim · model-written summary
Ethylene responsive elements were observed in 12 PpEXPs.
- Taxon
- peachPrunus persicae7
- Outcome
- ethylene responsive elementse0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene responsive elements were observed in 12 PpEXPs”
e7“peach genome”
Claim · model-written summary
Fifteen of the 26 PpEXPs were expressed in the fruit.
- Taxon
- peachPrunus persicae7
- Organ / stage
- fruite0
- Outcome
- expressed in the fruite0
- Value
- 15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“15 PpEXPs were expressed in the fruit”
e7“peach genome”
Claim · model-written summary
PpEXPs displayed distinct expression patterns in peach fruit varieties MF, NMF and SH at different stages.
- Taxon
- peachPrunus persicae7
- Material
- MF (melting), NMF (non-melting) and SH (stony hard)e0
- Outcome
- distinct expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcript profiling of PpEXPs in the peach fruit varieties of MF (melting), NMF (non-melting) and SH (stony hard) at different stages showed that PpEXPs displayed distinct expression patterns”
e7“peach genome”
Claim · model-written summary
PpEXPA7, PpEXPA13 and PpEXPA15 were selected as candidate genes because their expression was highly consistent with that of PpPGM, PpPGF and PpYUC11 for fruit texture.
- Taxon
- peachPrunus persicae7
- Gene
- PpEXPA7, PpEXPA13 and PpEXPA15e0
- Outcome
- candidate genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PpEXPA7, PpEXPA13 and PpEXPA15 were selected as candidate genes, as they were highly consistent with the patterns of previous reported key genes (PpPGM, PpPGF and PpYUC11)”
e7“peach genome”
Claim · model-written summary
A module identified as PpEXPA1 and PpEXPA7 was negatively correlated with fruit firmness.
- Taxon
- peachPrunus persicae7
- Gene
- PpEXPA1 and PpEXPA7e0
- Outcome
- phenotype of fruit firmnesse0
- Direction
- negatively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one module, with pink and midnightblue, negatively correlated with the phenotype of fruit firmness and was identified as PpEXPA1 and PpEXPA7”
e7“peach genome”
Claim · model-written summary
PpERF in the pink module might affect fruit texture development by regulating PpEXPs.
- Taxon
- peachPrunus persicae7
- Gene
- PpERFe0
- Outcome
- fruit texture developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the other module was identified as PpERF in the pink module, which might potentially effect fruit texture development by regulating PpEXPs”
e7“peach genome”