Abstract
Bacterial wilt, caused by Ralstonia solanacearum, is a destructive disease that causes significant loss in ginger productivity. This disease is notoriously difficult to control by chemical measures, prompting the exploration of biological control as a more sustainable and effective alternative strategy. In our research, we aimed to develop effective microbial agents to combat R. solanacearum-induced bacterial wilt in ginger. Initially, 307 Bacillus-like strains were isolated from ginger rhizosphere soil. Among these, ten strains exhibited significant antibacterial activity after extensive in vitro screening, with Bacillus-like isolate F18 being the most effective. Further tests in a co-culture assay revealed six isolates, notably L70, that significantly reduced the pathogen's fitness. To further assess their effectiveness, six isolates of L3, L72, L73, F18, HC-5, and L70 were then subjected to a hydroponics assay, where they substantially lowered disease incidence in ginger seedlings, especially strains F18, HC-5, and L73. These three strains were identified as Bacillus velezensis, exhibiting beneficial traits such as IAA production, siderophore secretion, and phosphate and potassium solubilization. Additionally, they also harbored genes related to the production of the dipeptide bacilysin and the polyketides macrolactin, bacillaene and difficidin. In greenhouse experiments, these three B. velezensis strains, particularly F18 and HC-5, demonstrated remarkable efficacy in controlling bacterial wilt. Tubers soaked in these biocontrol agents showed reduced disease severity and pathogen population. Moreover, the treatments promoted ginger growth, notably in tuber fresh weight, with HC-5 showing the greatest improvement. Conclusively, this study suggests a highly efficient strategy for biologically controlling ginger bacterial wilt, with B. velezensis strains HC-5, L73, and F18 emerging as promising biocontrol agents.
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Claims 15
Claim · model-written summary
Ten Bacillus-like isolates showed significant antibacterial activity in vitro, with F18 most effective.
Not reportedTaxon
Verbatim
e0“ten strains exhibited significant antibacterial activity after extensive in vitro screening, with Bacillus-like isolate F18 being the most effective”
Claim · model-written summary
Six isolates, notably L70, significantly reduced pathogen fitness in co-culture.
Not reportedTaxonComparator
Verbatim
e0“six isolates, notably L70, that significantly reduced the pathogen's fitness”
Claim · model-written summary
L3 substantially lowered disease incidence in ginger seedlings in hydroponics.
- Taxon
- ginger
Zingiber officinalee0 - Treatment
- L3e0
- Outcome
- disease incidencee0
- Direction
- lowerede0
- Setting
- hydroponics assaye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six isolates of L3, L72, L73, F18, HC-5, and L70 were then subjected to a hydroponics assay, where they substantially lowered disease incidence in ginger seedlings”
Claim · model-written summary
L72 substantially lowered ginger seedling disease incidence in hydroponics.
- Taxon
- gingerZingiber officinalee0
- Treatment
- L72e0
- Outcome
- disease incidencee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six isolates of L3, L72, L73, F18, HC-5, and L70 were then subjected to a hydroponics assay, where they substantially lowered disease incidence in ginger seedlings”
Claim · model-written summary
L73 was particularly effective at lowering ginger seedling disease incidence.
- Taxon
- gingerZingiber officinalee0
- Treatment
- L73e0
- Outcome
- disease incidencee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“where they substantially lowered disease incidence in ginger seedlings, especially strains F18, HC-5, and L73”
Claim · model-written summary
F18 was particularly effective at lowering ginger seedling disease incidence.
- Taxon
- gingerZingiber officinalee0
- Treatment
- F18e0
- Outcome
- disease incidencee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“where they substantially lowered disease incidence in ginger seedlings, especially strains F18, HC-5, and L73”
Claim · model-written summary
HC-5 was particularly effective at lowering ginger seedling disease incidence.
- Taxon
- gingerZingiber officinalee0
- Treatment
- HC-5e0
- Outcome
- disease incidencee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“where they substantially lowered disease incidence in ginger seedlings, especially strains F18, HC-5, and L73”
Claim · model-written summary
L70 substantially lowered ginger seedling disease incidence in hydroponics.
- Taxon
- gingerZingiber officinalee0
- Treatment
- L70e0
- Outcome
- disease incidencee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six isolates of L3, L72, L73, F18, HC-5, and L70 were then subjected to a hydroponics assay, where they substantially lowered disease incidence in ginger seedlings”
Claim · model-written summary
F18, HC-5 and L73 were identified as Bacillus velezensis and had IAA production, siderophore secretion and phosphate and potassium solubilization traits.
- Interactor
- Bacillus velezensis
Bacillus velezensise0biocontrol_agent - Outcome
- IAA production, siderophore secretion, and phosphate and potassium solubilizatione0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These three strains were identified as Bacillus velezensis, exhibiting beneficial traits such as IAA production, siderophore secretion, and phosphate and potassium solubilization”
Claim · model-written summary
The three Bacillus velezensis strains harbored genes for bacilysin, macrolactin, bacillaene and difficidin production.
- Outcome
- genes related to the production of the dipeptide bacilysin and the polyketides macrolactin, bacillaene and difficidine0
Not reportedTaxon
Verbatim
e0“they also harbored genes related to the production of the dipeptide bacilysin and the polyketides macrolactin, bacillaene and difficidin”
Claim · model-written summary
The three B. velezensis strains controlled bacterial wilt in greenhouse experiments, especially F18 and HC-5.
Not reportedTaxonComparator
Verbatim
e0“these three B. velezensis strains, particularly F18 and HC-5, demonstrated remarkable efficacy in controlling bacterial wilt”
Claim · model-written summary
Soaking tubers in biocontrol agents reduced disease severity.
- Organ / stage
- Tuberse0
- Treatment
- soaked in these biocontrol agentse0
- Outcome
- disease severitye0
- Direction
- reducede0
Not reportedTaxonComparator
Verbatim
e0“Tubers soaked in these biocontrol agents showed reduced disease severity and pathogen population”
Claim · model-written summary
Soaking tubers in biocontrol agents reduced pathogen population.
- Organ / stage
- Tuberse0
- Treatment
- soaked in these biocontrol agentse0
- Outcome
- pathogen populatione0
- Direction
- reducede0
Not reportedTaxonComparator
Verbatim
e0“Tubers soaked in these biocontrol agents showed reduced disease severity and pathogen population”
Claim · model-written summary
Biocontrol treatments promoted ginger tuber fresh weight, with HC-5 greatest.
- Taxon
- gingerZingiber officinalee0
- Treatment
- HC-5e0
- Outcome
- tuber fresh weighte0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the treatments promoted ginger growth, notably in tuber fresh weight, with HC-5 showing the greatest improvement”
Claim · model-written summary
The authors proposed HC-5, L73 and F18 as biocontrol agents for ginger bacterial wilt.
- Outcome
- promising biocontrol agentse0
Not reportedTaxon
Verbatim
e0“B. velezensis strains HC-5, L73, and F18 emerging as promising biocontrol agents”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.