Abstract
This study aimed to investigate the impact of selenium (Se) treatment on the accumulation of reactive oxygen species (ROS) and the expressions of related genes in broccoli. To achieve this, one group of broccoli heads was treated with a selenite solution of 2 mg L−1, while another group was soaked in distilled water, serving as the control. The effects of these treatments were evaluated by analyzing the browning, hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents, enzyme activity, and gene expression levels of WARK and RBOH. Our results show that the Se treatment effectively inhibited H2O2 accumulation in the broccoli and reduced harmful MDA levels. The inhibition of ROS accumulation following the Se treatment was associated with enhanced activity of the CAT and SOD enzymes, increased expression levels of BoCAT and BoSOD, and decreased expression levels of the WRKY and RBOH transcription factors. Our study provides insights into the mechanism of action of selenium and its potential application in vegetable storage.
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Claims 5
Claim · model-written summary
Selenium treatment inhibited H2O2 accumulation in broccoli compared with distilled water.
- Taxon
- broccoli
Brassica oleracea var. italicae2 - Treatment
- selenite solution of 2 mg L−1e1
- Outcome
- H2O2 accumulatione2
- Direction
- inhibitede2
- Comparator
- distilled watere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“one group of broccoli heads was treated with a selenite solution of 2 mg L−1, while another group was soaked in distilled water, serving as the control”
e2“the Se treatment effectively inhibited H2O2 accumulation in the broccoli and reduced harmful MDA levels”
Claim · model-written summary
Selenium treatment reduced harmful MDA levels in broccoli compared with distilled water.
- Taxon
- broccoliBrassica oleracea var. italicae2
- Treatment
- Se treatmente2
- Outcome
- harmful MDA levelse2
- Direction
- reducede2
- Comparator
- distilled watere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“one group of broccoli heads was treated with a selenite solution of 2 mg L−1, while another group was soaked in distilled water, serving as the control”
e2“the Se treatment effectively inhibited H2O2 accumulation in the broccoli and reduced harmful MDA levels”
Claim · model-written summary
Inhibition of ROS accumulation after selenium treatment was associated with enhanced activity of the CAT and SOD enzymes in broccoli.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- Se treatmente3
- Gene
- CAT and SODe3
- Outcome
- activitye3
- Direction
- enhancede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The inhibition of ROS accumulation following the Se treatment was associated with enhanced activity of the CAT and SOD enzymes, increased expression levels of BoCAT and BoSOD, and decreased expression levels of the WRKY and RBOH transcription factors”
e1“one group of broccoli heads was treated with a selenite solution of 2 mg L−1, while another group was soaked in distilled water, serving as the control”
Claim · model-written summary
Selenium treatment increased the expression levels of BoCAT and BoSOD in broccoli compared with distilled water.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- Se treatmente3
- Gene
- BoCAT and BoSODe3
- Outcome
- expression levelse3
- Direction
- increasede3
- Comparator
- distilled watere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The inhibition of ROS accumulation following the Se treatment was associated with enhanced activity of the CAT and SOD enzymes, increased expression levels of BoCAT and BoSOD, and decreased expression levels of the WRKY and RBOH transcription factors”
e1“one group of broccoli heads was treated with a selenite solution of 2 mg L−1, while another group was soaked in distilled water, serving as the control”
Claim · model-written summary
Selenium treatment decreased the expression levels of WRKY and RBOH transcription factors in broccoli compared with distilled water.
- Taxon
- broccoliBrassica oleracea var. italicae1
- Treatment
- Se treatmente3
- Gene
- WRKY and RBOHe3
- Outcome
- expression levelse3
- Direction
- decreasede3
- Comparator
- distilled watere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The inhibition of ROS accumulation following the Se treatment was associated with enhanced activity of the CAT and SOD enzymes, increased expression levels of BoCAT and BoSOD, and decreased expression levels of the WRKY and RBOH transcription factors”
e1“one group of broccoli heads was treated with a selenite solution of 2 mg L−1, while another group was soaked in distilled water, serving as the control”
The same questions in other papers
selenium atom · physiology: oxidative · Brassica oleracea (wild cabbage)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.