Abstract
Brown rot symptoms may be linked to alterations in the gene expression pattern of genes associated with cell wall degradation. In this study, we identify key carbohydrate-active enzymes (CAZymes) involved in cell wall degradation by Monilinia fructicola, including pme2 and pme3 (pectin methylesterases), cut1 (cutinase) and nep2 (necrosis-inducing factor). The expression of these genes is significantly modulated by red and blue light during early nectarine infection. The polygalacturonase gene pg1 and the cellulase gene cel1 also exhibit photoinduction albeit to a lesser extent. Red and blue light cause an acceleration in the initial stages of brown rot development caused by M. fructicola on nectarines. Disease symptoms like tissue maceration were evident after an incubation period of 24 h followed by 14 h of light exposition, in contrast to the usual incubation period of 48 to 72 h. Furthermore, the culture media exerts an impact on gene regulation, suggesting a complex interplay between light and nutrient signalling pathways in M. fructicola. In addition, we observe that red light promotes colony growth on a 12 h photoperiod and consistently reduces conidiation. In contrast, blue light hampers growth rate on both the 12 h and the 8 h photoperiod but only diminishes conidiation on the 12 h photoperiod. These findings enhance our comprehension of genes associated with cell wall degradation and the environmental factors influencing brown rot development.
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Claims 10
Claim · model-written summary
Key CAZymes involved in Monilinia cell wall degradation were identified.
- Taxon
- nectarine
Prunus persica var. nucipersicat0 - Interactor
- Monilinia fructicola
Monilinia fructicolae0pathogen - Gene
- pme2 and pme3e0
- Outcome
- cell wall degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identify key carbohydrate-active enzymes (CAZymes) involved in cell wall degradation by Monilinia fructicola, including pme2 and pme3 (pectin methylesterases), cut1 (cutinase) and nep2 (necrosis-inducing factor)”
t0“nectarine”
Claim · model-written summary
Red and blue light significantly modulated expression of the named fungal genes in early infection.
- Taxon
- nectarinePrunus persica var. nucipersicae0
- Treatment
- red and blue lighte0
- Outcome
- expression of these genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of these genes is significantly modulated by red and blue light during early nectarine infection”
Claim · model-written summary
The fungal pg1 and cel1 genes also showed weaker photoinduction.
- Taxon
- nectarinePrunus persica var. nucipersicat0
- Gene
- pg1 and the cellulase gene cel1e0
- Outcome
- photoinductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polygalacturonase gene pg1 and the cellulase gene cel1 also exhibit photoinduction albeit to a lesser extent”
t0“nectarine”
Claim · model-written summary
Red and blue light accelerated initial brown rot development on nectarines.
- Taxon
- nectarinesPrunus persica var. nucipersicae0
- Treatment
- Red and blue lighte0
- Interactor
- M. fructicolaMonilinia fructicolae0pathogen
- Outcome
- brown rot developmente0
- Direction
- acceleratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Red and blue light cause an acceleration in the initial stages of brown rot development caused by M. fructicola on nectarines”
Claim · model-written summary
Tissue maceration appeared after 24 hours plus 14 hours of light, earlier than usual.
- Taxon
- nectarinePrunus persica var. nucipersicat0
- Outcome
- tissue maceratione0
- Value
- 24 h followed by 14 he0
- Qualifier
- 48 to 72 he0
- Comparator
- usual incubation periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disease symptoms like tissue maceration were evident after an incubation period of 24 h followed by 14 h of light exposition, in contrast to the usual incubation period of 48 to 72 h”
t0“nectarine”
Claim · model-written summary
Culture medium affected fungal gene regulation.
- Taxon
- nectarinePrunus persica var. nucipersicat0
- Treatment
- culture mediae0
- Outcome
- gene regulatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the culture media exerts an impact on gene regulation”
t0“nectarine”
Claim · model-written summary
Red light promoted fungal colony growth at a 12 hour photoperiod.
- Taxon
- nectarinePrunus persica var. nucipersicat0
- Treatment
- red lighte0
- Outcome
- colony growthe0
- Direction
- promotese0
- Qualifier
- 12 h photoperiode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“red light promotes colony growth on a 12 h photoperiod”
t0“nectarine”
Claim · model-written summary
Red light reduced fungal conidiation.
- Taxon
- nectarinePrunus persica var. nucipersicat0
- Treatment
- red lighte0
- Outcome
- conidiatione0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“red light promotes colony growth on a 12 h photoperiod and consistently reduces conidiation”
t0“nectarine”
Claim · model-written summary
Blue light hampered fungal growth at both tested photoperiods.
- Taxon
- nectarinePrunus persica var. nucipersicat0
- Treatment
- blue lighte0
- Outcome
- growth ratee0
- Direction
- hamperse0
- Qualifier
- both the 12 h and the 8 h photoperiode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blue light hampers growth rate on both the 12 h and the 8 h photoperiod”
t0“nectarine”
Claim · model-written summary
Blue light diminished fungal conidiation only at a 12 hour photoperiod.
- Taxon
- nectarinePrunus persica var. nucipersicat0
- Treatment
- blue lighte0
- Outcome
- conidiatione0
- Direction
- diminishese0
- Qualifier
- 12 h photoperiode0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“blue light hampers growth rate on both the 12 h and the 8 h photoperiod but only diminishes conidiation on the 12 h photoperiod”
t0“nectarine”
The same questions in other papers
light · Monilinia fructicola · Prunus persica (peach)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.