Abstract
Light represents a powerful signal for the regulation of virulence in many microbial pathogens. Monilinia fructicola is the most virulent species causing brown rot in stone fruit crops. To understand the influence of light on M. fructicola, we measured the effect of white light and photoperiods on the colonial growth and sporulation of the model M. fructicola strain 38C on solid cultures. Searches in the M. fructicola 38C genome predicted a complete set of genes coding for photoreceptors possibly involved in the perception of all ranges of wavelengths. Since white light had an obvious negative effect on vegetative growth and the asexual development of M. fructicola 38C on potato dextrose agar, we studied how light influences photoresponse genes in M. fructicola during early peach infection and in liquid culture. The transcriptomes were analyzed in “Red Jim” nectarines infected by M. fructicola 38C and subjected to light pulses for 5 min and 14 h after 24 h of incubation in darkness. Specific light-induced genes were identified. Among these, we confirmed in samples from infected fruit or synthetic media that blue light photoreceptor vvd1 was among the highest expressed genes. An unknown gene, far1, coding for a small protein conserved in many families of Ascomycota phylum, was also highly induced by light. In contrast, a range of well-known photoreceptors displayed a low transcriptional response to light in M. fructicola from nectarines but not on the pathogen mycelium growing in liquid culture media for 6 days.
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Claims 6
Claim · model-written summary
Genome searches predicted a complete photoreceptor gene set in the pathogen.
- Taxon
- nectarines
Prunus persica var. nucipersicae1 - Interactor
- M. fructicola 38C
Monilinia fructicolae0pathogen - Outcome
- complete set of genes coding for photoreceptorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Searches in the M. fructicola 38C genome predicted a complete set of genes coding for photoreceptors possibly involved in the perception of all ranges of wavelengths”
t0“Monilinia fructicola Response to White Light”
e1“The transcriptomes were analyzed in “Red Jim” nectarines infected by M. fructicola 38C and subjected to light pulses for 5 min and 14 h after 24 h of incubation in darkness. Specific light-induced genes were identified.”
Claim · model-written summary
White light negatively affected pathogen vegetative growth and asexual development on agar.
- Taxon
- nectarinesPrunus persica var. nucipersicae1
- Treatment
- white lighte0
- Interactor
- M. fructicola 38CMonilinia fructicolae0pathogen
- Outcome
- vegetative growth and the asexual development of M. fructicola 38Ce0
- Direction
- negative effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“white light had an obvious negative effect on vegetative growth and the asexual development of M. fructicola 38C on potato dextrose agar”
t0“Monilinia fructicola Response to White Light”
e1“The transcriptomes were analyzed in “Red Jim” nectarines infected by M. fructicola 38C and subjected to light pulses for 5 min and 14 h after 24 h of incubation in darkness. Specific light-induced genes were identified.”
Claim · model-written summary
Light induced specific pathogen genes in infected nectarines.
- Taxon
- nectarinesPrunus persica var. nucipersicae0
- Material
- “Red Jim”e0
- Treatment
- light pulsese0
- Interactor
- M. fructicola 38CMonilinia fructicolae0pathogen
- Outcome
- light-induced genese0
- Direction
- light-inducede0
- Comparator
- darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptomes were analyzed in “Red Jim” nectarines infected by M. fructicola 38C and subjected to light pulses for 5 min and 14 h after 24 h of incubation in darkness. Specific light-induced genes were identified.”
Claim · model-written summary
The pathogen blue light photoreceptor vvd1 was among the highest expressed genes in infected fruit or media.
- Taxon
- nectarinesPrunus persica var. nucipersicae1
- Interactor
- Monilinia fructicolaMonilinia fructicolat0pathogen
- Gene
- vvd1e0
- Outcome
- highest expressed genese0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we confirmed in samples from infected fruit or synthetic media that blue light photoreceptor vvd1 was among the highest expressed genes”
t0“Monilinia fructicola Response to White Light”
e1“The transcriptomes were analyzed in “Red Jim” nectarines infected by M. fructicola 38C and subjected to light pulses for 5 min and 14 h after 24 h of incubation in darkness. Specific light-induced genes were identified.”
Claim · model-written summary
Light highly induced the pathogen gene far1.
- Taxon
- nectarinesPrunus persica var. nucipersicae1
- Treatment
- lighte0
- Interactor
- Monilinia fructicolaMonilinia fructicolat0pathogen
- Gene
- far1e0
- Outcome
- far1e0
- Direction
- highly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An unknown gene, far1, coding for a small protein conserved in many families of Ascomycota phylum, was also highly induced by light”
t0“Monilinia fructicola Response to White Light”
e1“The transcriptomes were analyzed in “Red Jim” nectarines infected by M. fructicola 38C and subjected to light pulses for 5 min and 14 h after 24 h of incubation in darkness. Specific light-induced genes were identified.”
Claim · model-written summary
Known pathogen photoreceptors responded weakly to light in nectarines but not in liquid culture.
- Taxon
- nectarinesPrunus persica var. nucipersicae0
- Treatment
- lighte0
- Interactor
- M. fructicolaMonilinia fructicolae0pathogen
- Outcome
- transcriptional responsee0
- Direction
- lowe0
- Comparator
- pathogen mycelium growing in liquid culture mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a range of well-known photoreceptors displayed a low transcriptional response to light in M. fructicola from nectarines but not on the pathogen mycelium growing in liquid culture media for 6 days”
The same questions in other papers
light · Monilinia fructicola · Prunus persica (peach)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.