Abstract
Soil legacy phosphorus (P) accumulated due to the long-term continuous application of P fertilizers in agricultural fields accumulated in the soil for years, as sparingly soluble forms. This can be made as a possible soil P source using P-activators. In this study, a laboratory incubation and a pot experiment were conducted to evaluate the potential of some P activators (Phosphorus solubilizing bacteria, phytase, humic acid, oxalic acid) in increasing the availability of residual phosphorus in calcareous soil. For the incubation experiment, the P activators were applied alone and in combination. Soil samples were analyzed at subsequent intervals for soil available P, alkaline phosphatase activity, and sequential P fractions. The results showed that phytase application with humic acid (HA) increased the available soil P (12.4 kg ha−1) and alkaline phosphatase activity (47.42 µg p-nitrophenol released g−1 soil hr−1) followed by phosphorus solubilizing Bacteria (PSB) with humic acid and PSB with phytase treatments. All treatments have a significant effect on different soil P-fractions. A greater reduction in calcium phosphate fraction was noted in phytase and humic acid application. The combined application of P-activators performed better than their sole application. The best three treatments selected from the laboratory incubated experiment were used for the pot experiment with maize, along with the different dosages of P fertilizer. The morphological parameters were observed on the 30th and 60th days of the maize crop. The results of plant height, chlorophyll index, and dry matter production showed that the application of phytase and HA with 100% recommended dose of P was statistically comparable with phytase and HA with 75% recommended dose of P. The findings have illustrated the extent of P release by different P activators in increasing the legacy P availability. The application of phytase and HA with 25% reduced dose of P fertilizers will promote the availability of legacy P in calcareous soils through solubilization.
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Claims 8
Claim · model-written summary
In the incubation experiment, phytase with humic acid increased available soil P to 12.4 kg ha-1.
- Treatment
- phytase application with humic acid (HA)e0
- Outcome
- available soil Pe0
- Direction
- increasede0
- Value
- 12.4 kg ha−1e0
Not reportedTaxonComparatorSetting
Verbatim
e0“phytase application with humic acid (HA) increased the available soil P (12.4 kg ha−1) and alkaline phosphatase activity (47.42 µg p-nitrophenol released g−1 soil hr−1)”
Claim · model-written summary
In the incubation experiment, phytase with humic acid increased alkaline phosphatase activity to 47.42 ug p-nitrophenol released per g soil per hour.
- Treatment
- phytase application with humic acid (HA)e0
- Outcome
- alkaline phosphatase activitye0
- Direction
- increasede0
- Value
- 47.42 µg p-nitrophenol released g−1 soil hr−1e0
Not reportedTaxonComparator
Verbatim
e0“phytase application with humic acid (HA) increased the available soil P (12.4 kg ha−1) and alkaline phosphatase activity (47.42 µg p-nitrophenol released g−1 soil hr−1)”
Claim · model-written summary
Phosphorus solubilizing bacteria with humic acid and PSB with phytase ranked next after phytase with humic acid for available P and phosphatase activity.
- Treatment
- phosphorus solubilizing Bacteria (PSB) with humic acid and PSB with phytase treatmentse0
- Outcome
- followed bye0
- Direction
- followed bye0
Not reportedTaxon
Verbatim
e0“followed by phosphorus solubilizing Bacteria (PSB) with humic acid and PSB with phytase treatments”
Claim · model-written summary
All P-activator treatments had a significant effect on the different soil P fractions.
Not reportedTaxonComparatorDirection
Verbatim
e0“All treatments have a significant effect on different soil P-fractions”
Claim · model-written summary
Phytase and humic acid application caused a greater reduction in the calcium phosphate fraction.
- Treatment
- phytase and humic acid applicatione0
- Outcome
- calcium phosphate fractione0
- Direction
- greater reductione0
Not reportedTaxonComparator
Verbatim
e0“A greater reduction in calcium phosphate fraction was noted in phytase and humic acid application”
Claim · model-written summary
Combined application of P-activators performed better than their sole application.
- Treatment
- combined application of P-activatorse0
- Outcome
- performed bettere0
- Direction
- performed bettere0
- Comparator
- their sole applicatione0
Not reportedTaxon
Verbatim
e0“The combined application of P-activators performed better than their sole application”
Claim · model-written summary
In the maize pot experiment, plant height, chlorophyll index and dry matter with phytase and HA at 100% recommended P dose were statistically comparable with phytase and HA at 75% of the dose.
- Treatment
- phytase and HA with 100% recommended dose of Pe0
- Outcome
- plant height, chlorophyll index, and dry matter productione0
- Direction
- statistically comparablee0
- Comparator
- phytase and HA with 75% recommended dose of Pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize Growth”
e0“The results of plant height, chlorophyll index, and dry matter production showed that the application of phytase and HA with 100% recommended dose of P was statistically comparable with phytase and HA with 75% recommended dose of P”
Claim · model-written summary
Phytase and humic acid with a 25% reduced P fertilizer dose will promote availability of legacy P in calcareous soils through solubilization.
Not reportedTaxon
Verbatim
e0“The application of phytase and HA with 25% reduced dose of P fertilizers will promote the availability of legacy P in calcareous soils through solubilization”
The same questions in other papers
other · physiology: pigments · Zea mays (maize)Positive and negative results
2 papers in the same direction as this paper · 27 reporting no difference from an alternative
- positive
- positive
- positive
26 papers more in this group
See all 30 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.