Abstract
Turmeric (Curcuma longa) is used as a spice and has excellent medicinal value. However, the field multiplication rate of turmeric plants is not satisfactory. In vitro methods could be a feasible alternative to vegetative propagation in the field. This study was aimed at developing a suitable protocol for the clonal propagation of turmeric confirming the genetic fidelity of the in vitro regenerated plantlets. The explants of shoot bud (full, half, and quarter), root, rhizome, and leaf were cultivated on MS medium with BAP, NAA, and Kinetin. Cultured shoot buds showed a higher regeneration frequency and shoots per explant than those obtained from other explants. The highest shoot regeneration was 72.49% from shoot buds, followed by rhizome (68.76%) and the lowest was 53.93 % from ¼ shoot buds. There were significant effects of growth regulator combinations and concentrations in shoot regeneration from shoot bud explant. The highest shoot regeneration per shoot bud explant was 4.42 with BAP at 20.0 mg/l and NAA at 0.5 mg/l at 24 weeks. The best rooting response with 9.75 roots per explant was reported with 1.0 mg/l BAP with 1.0 mg/l NAA. The rooted plants were successfully transferred to pots for their acclimatization. Both the micropropagated shoots and their mother plant produced monomorphic bands with the six RAPD markers that confirm the genetic fidelity of micropropagated clones. These results showed that in vitro raised plantlets of turmeric had no risk of somaclonal variations and were found to be true-to-type in nature over the culture period. The protocol developed through this investigation has the potential application for the rapid multiplication of turmeric plants. Plant Tissue Cult. & Biotech. 34(1): 55-69, 2024 (June)
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Claims 11
Claim · model-written summary
Shoot bud cultures exceeded other explants in regeneration frequency and shoots per explant.
- Taxon
- Turmeric (Curcuma longa)
_W_IMG_2440.jpg?width=60)
Curcuma longat0 - Treatment
- Cultured shoot budse0
- Outcome
- regeneration frequency and shoots per explante0
- Direction
- highere0
- Comparator
- other explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_W_IMG_2440.jpg?width=500)
Verbatim
t0“Turmeric (Curcuma longa)”
e0“Cultured shoot buds showed a higher regeneration frequency and shoots per explant than those obtained from other explants”
Claim · model-written summary
Shoot buds showed 72.49% regeneration.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Organ / stage
- shoot budse0
- Outcome
- shoot regeneratione0
- Value
- 72.49%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“highest shoot regeneration was 72.49% from shoot buds”
Claim · model-written summary
Rhizomes showed 68.76% regeneration.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Outcome
- rhizomee0
- Value
- 68.76%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“followed by rhizome (68.76%)”
Claim · model-written summary
Quarter shoot buds had the lowest regeneration at 53.93%.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Outcome
- ¼ shoot budse0
- Direction
- loweste0
- Value
- 53.93 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“lowest was 53.93 % from ¼ shoot buds”
Claim · model-written summary
Regulator combination and concentration significantly affected shoot regeneration.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Treatment
- growth regulator combinations and concentrationse0
- Outcome
- shoot regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“significant effects of growth regulator combinations and concentrations in shoot regeneration from shoot bud explant”
Claim · model-written summary
BAP and NAA gave 4.42 shoots per explant at 24 weeks.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Treatment
- BAP at 20.0 mg/l and NAA at 0.5 mg/le0
- Outcome
- shoot regeneration per shoot bud explante0
- Direction
- higheste0
- Value
- 4.42e0
- Qualifier
- 24 weekse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“highest shoot regeneration per shoot bud explant was 4.42 with BAP at 20.0 mg/l and NAA at 0.5 mg/l at 24 weeks”
Claim · model-written summary
Equal BAP and NAA concentrations yielded 9.75 roots per explant.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Treatment
- 1.0 mg/l BAP with 1.0 mg/l NAAe0
- Outcome
- rooting responsee0
- Direction
- beste0
- Value
- 9.75 roots per explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“best rooting response with 9.75 roots per explant was reported with 1.0 mg/l BAP with 1.0 mg/l NAA”
Claim · model-written summary
Rooted plants were transferred to pots successfully.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Organ / stage
- rooted plantse0
- Outcome
- successfully transferred to potse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“The rooted plants were successfully transferred to pots for their acclimatization”
Claim · model-written summary
Six RAPD markers gave monomorphic bands in clones and mother plants.
- Taxon
- Turmeric (Curcuma longa)Curcuma longat0
- Outcome
- monomorphic bandse0
- Value
- six RAPD markerse0
- Comparator
- mother plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“Both the micropropagated shoots and their mother plant produced monomorphic bands with the six RAPD markers that confirm the genetic fidelity of micropropagated clones”
Claim · model-written summary
Authors judged regenerated plants true-to-type without somaclonal variation.
- Taxon
- turmericCurcuma longae0
- Outcome
- true-to-type in naturee0
- Qualifier
- over the culture periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“in vitro raised plantlets of turmeric had no risk of somaclonal variations and were found to be true-to-type in nature over the culture period”
Claim · model-written summary
The protocol has potential for rapid turmeric multiplication.
- Taxon
- turmeric plantsCurcuma longae0
- Outcome
- rapid multiplication of turmeric plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Turmeric (Curcuma longa)”
e0“protocol developed through this investigation has the potential application for the rapid multiplication of turmeric plants”
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