Abstract
Maize is an important food and feed crop. Under limited arable land area, the cultivation of high-density-tolerance crops is a key factor in promoting yield improvement. Leaf width and stalk strength are important influences on density tolerance in maize. However, no comprehensive transcriptomic analysis has focused on maize’s leaf width and stalk strength formation mechanisms. In this study, comparative transcriptomic analyses demonstrated that significant transcriptome changes occurred regarding leaf width and stalk strength of narrow-leaved and wide-leaved maize inbred lines, with a total of 5001 differentially expressed genes (DEGs) identified. Enrichment analysis showed that phenylpropanoid biosynthesis, starch and sucrose metabolism, phytohormone signaling, amino acid metabolism, and brassinosteroid biosynthesis were significantly correlated with the formation of maize leaf shape and stalk strength and that the genes in these pathways were primarily involved in cell wall formation. Weighted gene co-expression network analysis identified 2 modules significantly correlated with leaf width and stalk strength, from which 11 hub genes were further identified. The 11 leaf and stem development genes in different pathways were validated using qRT-PCR. These findings can provide a theoretical basis for the mechanism of narrow-leaf and high-strength stalk formation in high-density-tolerance maize and contribute to the proposal of a breeding strategy for yield improvement.
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Claims 6
Claim · model-written summary
Narrow- and wide-leaved maize inbred lines differed in transcriptomes, with 5001 differentially expressed genes identified.
- Material
- narrow-leaved and wide-leaved maize inbred linese0
- Outcome
- differentially expressed genes (DEGs)e0
- Direction
- significant transcriptome changese0
- Value
- 5001e0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant transcriptome changes occurred regarding leaf width and stalk strength of narrow-leaved and wide-leaved maize inbred lines, with a total of 5001 differentially expressed genes (DEGs) identified”
Claim · model-written summary
Phenylpropanoid biosynthesis, starch and sucrose metabolism, and phytohormone signaling were significantly correlated with maize leaf shape and stalk strength formation.
- Organ / stage
- leafe0
- Outcome
- formation of maize leaf shape and stalk strengthe0
- Qualifier
- phenylpropanoid biosynthesis, starch and sucrose metabolism, phytohormone signaling, amino acid metabolism, and brassinosteroid biosynthesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenylpropanoid biosynthesis, starch and sucrose metabolism, phytohormone signaling, amino acid metabolism, and brassinosteroid biosynthesis were significantly correlated with the formation of maize leaf shape and stalk strength”
Claim · model-written summary
Genes in the enriched pathways were primarily involved in cell wall formation.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genes in these pathways were primarily involved in cell wall formation”
t0“Maize Inbred Lines”
Claim · model-written summary
Coexpression analysis identified two modules significantly correlated with leaf width and stalk strength.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Weighted gene co-expression network analysis identified 2 modules significantly correlated with leaf width and stalk strength”
t0“Maize Inbred Lines”
Claim · model-written summary
Eleven hub genes were identified from the two modules.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from which 11 hub genes were further identified”
t0“Maize Inbred Lines”
Claim · model-written summary
Eleven leaf and stem development genes were validated by qRT-PCR.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 11 leaf and stem development genes in different pathways were validated using qRT-PCR”
t0“Maize Inbred Lines”