Abstract
Cultivated peanut (Arachis hypogaea L.) is a key oil- and protein-providing legume crop of the world. It is full of nutrients, and its nutrient profile is comparable to that of other nuts. Peanut is a unique plant as it showcases a pegging phenomenon, producing flowers above ground, and after fertilization, the developing peg enters the soil and produces seeds underground. This geocarpic nature of peanut exposes its seeds to soil pathogens. Peanut seeds are protected by an inedible pericarp and testa. The pericarp- and testa-specific promoters can be effectively used to improve the seed defense. We identified a pericarp- and testa-abundant expression gene (AhN8DT-2) from available transcriptome expression data, whose tissue-specific expression was further confirmed by the qRT-PCR. The 1827bp promoter sequence was used to construct the expression vector using the pMDC164 vector for further analysis. Quantitative expression of the GUS gene in transgenic Arabidopsis plants showed its high expression in the pericarp. GUS staining showed a deep blue color in the pericarp and testa. Cryostat sectioning of stained Arabidopsis seeds showed that expression is only limited to seed coat (testa), and staining was not present in cotyledons and embryos. GUS staining was not detected in any other tissues, including seedlings, leaves, stems, and roots, except for some staining in flowers. Under different phytohormones, this promoter did not show an increase in expression level. These results indicated that the AhN8DT-2 promoter drives GUS gene expression in a pericarp- and testa-specific manner. The identified promoter can be utilized to drive disease resistance genes, specifically in the pericarp and testa, enhancing peanut seed defense against soil-borne pathogens. This approach has broader implications for improving the resilience of peanut crops and other legumes, contributing to sustainable agricultural practices and food security.
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Claims 7
Claim · model-written summary
AhN8DT-2 was identified as abundantly expressed in pericarp and testa, with tissue specificity confirmed by qRT-PCR.
- Taxon
- Arachis hypogaea
Arachis hypogaeat0 - Organ / stage
- pericarp and testae1
- Gene
- AhN8DT-2e0
- Outcome
- pericarp- and testa-abundant expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified a pericarp- and testa-abundant expression gene (AhN8DT-2) from available transcriptome expression data, whose tissue-specific expression was further confirmed by the qRT-PCR.”
e1“GUS staining showed a deep blue color in the pericarp and testa.”
t0“Arachis hypogaea”
Claim · model-written summary
The AhN8DT-2 promoter drove high GUS expression in the pericarp of transgenic Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- pericarpe0
- Gene
- GUS genee0
- Outcome
- expression of the GUS genee0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative expression of the GUS gene in transgenic Arabidopsis plants showed its high expression in the pericarp.”
t0“AhN8DT-2”
Claim · model-written summary
GUS staining was deep blue in the pericarp and testa.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Organ / stage
- pericarp and testae0
- Gene
- GUSe0
- Outcome
- deep blue color in the pericarp and testae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS staining showed a deep blue color in the pericarp and testa.”
t0“Arachis hypogaea”
Claim · model-written summary
In stained Arabidopsis seeds, expression was limited to the testa and absent in cotyledons and embryos.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- seed coat (testa)e0
- Outcome
- expression is only limited to seed coat (testa), and staining was not present in cotyledons and embryose0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cryostat sectioning of stained Arabidopsis seeds showed that expression is only limited to seed coat (testa), and staining was not present in cotyledons and embryos.”
Claim · model-written summary
GUS staining was absent from seedlings, leaves, stems and roots, with some staining in flowers.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Gene
- GUSe0
- Outcome
- GUS staining was not detected in any other tissues, including seedlings, leaves, stems, and roots, except for some staining in flowerse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS staining was not detected in any other tissues, including seedlings, leaves, stems, and roots, except for some staining in flowers.”
t0“Arachis hypogaea”
Claim · model-written summary
Different phytohormones did not increase promoter expression.
- Taxon
- Arachis hypogaeaArachis hypogaeat0
- Treatment
- different phytohormonese0
- Outcome
- expression levele0
- Direction
- did not show an increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under different phytohormones, this promoter did not show an increase in expression level.”
t0“Arachis hypogaea”
Claim · model-written summary
The authors propose using the promoter to drive disease resistance genes in the pericarp and testa.
- Taxon
- peanutArachis hypogaeae0
- Organ / stage
- pericarp and testae0
- Gene
- disease resistance genese0
- Outcome
- drive disease resistance genes, specifically in the pericarp and testae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The identified promoter can be utilized to drive disease resistance genes, specifically in the pericarp and testa, enhancing peanut seed defense against soil-borne pathogens.”
The same questions in other papers
class · molecular: expression · Arachis hypogaea (peanut)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.