Abstract
BACKGROUND: Laccase (LAC) gene family plays a pivotal role in plant lignin biosynthesis and adaptation to various stresses. Limited research has been conducted on laccase genes in common beans. RESULTS: 29 LAC gene family members were identified within the common bean genome, distributed unevenly in 9 chromosomes. These members were divided into 6 distinct subclades by phylogenetic analysis. Further phylogenetic analyses and synteny analyses indicated that considerable gene duplication and loss presented throughout the evolution of the laccase gene family. Purified selection was shown to be the major evolutionary force through Ka / Ks. Transcriptional changes of PvLAC genes under low temperature and salt stress were observed, emphasizing the regulatory function of these genes in such conditions. Regulation by abscisic acid and gibberellins appears to be the case for PvLAC3, PvLAC4, PvLAC7, PvLAC13, PvLAC14, PvLAC18, PvLAC23, and PvLAC26, as indicated by hormone induction experiments. Additionally, the regulation of PvLAC3, PvLAC4, PvLAC7, and PvLAC14 in response to nicosulfuron and low-temperature stress were identified by virus-induced gene silence, which demonstrated inhibition on growth and development in common beans. CONCLUSIONS: The research provides valuable genetic resources for improving the resistance of common beans to abiotic stresses and enhance the understanding of the functional roles of the LAC gene family.
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Claims 7
Claim · model-written summary
Twenty-nine LAC family members were identified in the common bean genome across nine chromosomes.
- Taxon
- common bean (Phaseolus vulgaris)
Phaseolus vulgarist0 - Gene
- LACe0
- Outcome
- 29 LAC gene family memberse0
- Value
- 29e0
- Qualifier
- distributed unevenly in 9 chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris)”
e0“29 LAC gene family members were identified within the common bean genome, distributed unevenly in 9 chromosomes.”
Claim · model-written summary
The LAC members were divided into six subclades.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarist0
- Outcome
- 6 distinct subcladese0
- Value
- 6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris)”
e0“These members were divided into 6 distinct subclades by phylogenetic analysis.”
Claim · model-written summary
Gene duplication and loss occurred during laccase family evolution.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarist0
- Gene
- laccase gene familye0
- Outcome
- gene duplication and losse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris)”
e0“considerable gene duplication and loss presented throughout the evolution of the laccase gene family.”
Claim · model-written summary
Purifying selection was reported as the main force in laccase family evolution.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarist0
- Outcome
- Purified selectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris)”
e0“Purified selection was shown to be the major evolutionary force through Ka / Ks.”
Claim · model-written summary
PvLAC transcription changed under low temperature and salt stress.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarist0
- Treatment
- low temperature and salt stresse0
- Gene
- PvLACe0
- Outcome
- Transcriptional changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris)”
e0“Transcriptional changes of PvLAC genes under low temperature and salt stress were observed”
Claim · model-written summary
Hormone induction suggested regulation of eight PvLAC genes by abscisic acid and gibberellins.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarist0
- Treatment
- abscisic acid and gibberellinse0
- Gene
- PvLAC3, PvLAC4, PvLAC7, PvLAC13, PvLAC14, PvLAC18, PvLAC23, and PvLAC26e0
- Outcome
- Regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris)”
e0“Regulation by abscisic acid and gibberellins appears to be the case for PvLAC3, PvLAC4, PvLAC7, PvLAC13, PvLAC14, PvLAC18, PvLAC23, and PvLAC26, as indicated by hormone induction experiments.”
Claim · model-written summary
Silencing PvLAC3, PvLAC4, PvLAC7 and PvLAC14 inhibited common bean growth and development under nicosulfuron and cold stress.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarist0
- Treatment
- virus-induced gene silencee0
- Gene
- PvLAC3, PvLAC4, PvLAC7, and PvLAC14e0
- Outcome
- growth and developmente0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“common bean (Phaseolus vulgaris)”
e0“the regulation of PvLAC3, PvLAC4, PvLAC7, and PvLAC14 in response to nicosulfuron and low-temperature stress were identified by virus-induced gene silence, which demonstrated inhibition on growth and development in common beans.”
The same questions in other papers
loss of function · growth · Phaseolus vulgaris (common bean)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.