Abstract
AIMS: The objective of this study was to elucidate the role and mechanism of changes in the rhizosphere microbiome following Arthrobotrys oligospora treatment in the biological control of root-knot nematodes and identify the key fungal and bacterial species that collaborate with A. oligospora to biocontrol root-knot nematodes. METHODS AND RESULTS: We conducted a pot experiment to investigate the impact of A. oligospora treatment on the biocontrol efficiency of A. oligospora against Meloidogyne incognita infecting tomatoes. We analyzed the rhizosphere bacteria and fungi communities of tomato by high-throughput sequencing of the 16S rRNA gene fragment and the internal transcribed spacer (ITS). The results indicated that the application of A. oligospora resulted in a 53.6% reduction in the disease index of M. incognita infecting tomato plants. The bacterial diversity of rhizosphere soil declined in the A. oligospora-treated group, while fungal diversity increased. The A. oligospora treatment enriched the tomato rhizosphere with Acidobacteriota, Firmicutes, Bradyrhizobium, Sphingomonadales, Glomeromycota, and Purpureocillium. These organisms are involved in the utilization of rhizosphere organic matter, nitrogen, and glycerolipids, or play the role of ectomycorrhiza or directly kill nematodes. The networks of bacterial and fungal co-occurrence exhibited a greater degree of stability and complexity in the A. oligospora treatment group. CONCLUSIONS: This study demonstrated the key fungal and bacterial species that collaborate with the A. oligospora in controlling the root-knot nematode and elaborated the potential mechanisms involved. The findings offer valuable insights and inspiration for the advancement of bionematicide based on nematode-trapping fungi.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Arthrobotrys oligospora application reduced the M. incognita disease index in tomato by 53.6%.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- application of A. oligosporae0
- Interactor
- M. incognita
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogyne incognitae0pathogen - Second interactor
- A. oligosporae0biocontrol_agent
- Outcome
- disease indexe0
- Direction
- reductione0
- Value
- 53.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“application of A. oligospora resulted in a 53.6% reduction in the disease index of M. incognita infecting tomato plants.”
Claim · model-written summary
A. oligospora treatment decreased bacterial diversity in tomato rhizosphere soil.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- A. oligospora-treated groupe0
- Outcome
- bacterial diversity of rhizosphere soile0
- Direction
- declinede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bacterial diversity of rhizosphere soil declined in the A. oligospora-treated group”
t0“tomato”
Claim · model-written summary
A. oligospora treatment increased fungal diversity in tomato rhizosphere soil.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- fungal diversitye0
- Direction
- increasede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while fungal diversity increased.”
t0“tomato”
Claim · model-written summary
A. oligospora treatment enriched the tomato rhizosphere with six named microbial groups.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- A. oligospora treatmente0
- Outcome
- Acidobacteriota, Firmicutes, Bradyrhizobiume0
- Direction
- enrichede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The A. oligospora treatment enriched the tomato rhizosphere with Acidobacteriota, Firmicutes, Bradyrhizobium, Sphingomonadales, Glomeromycota, and Purpureocillium.”
Claim · model-written summary
Bacterial and fungal co-occurrence networks were more stable and complex after A. oligospora treatment.
Not reportedTaxonComparator
Verbatim
e0“The networks of bacterial and fungal co-occurrence exhibited a greater degree of stability and complexity in the A. oligospora treatment group.”