Abstract
High Resolution Image Download MS PowerPoint Slide Soybean cyst nematode (SCN, Heterodera glycines ) is a sedentary endoparasite nematode that results in severe economic losses in soybean crops. miRNAs play crucial roles in plant responses to nematode. However, the role of miR2119 responding to SCN stress in soybean. Here, we demonstrated that the transcript levels of polycistronic precursors containing miR2119 and miR398a were significantly reduced in soybean upon nematode infection. Promoter of the miR2119-398a precursor analysis was conducted containing a GUS reporter gene. GUS activity assays demonstrated a decrease in miR2119-398a promoter during SCN infection. Overexpression of polycistronic precursor miR2119-398a (OE-premiR2119-398a) and miR2119 precursor (OE-premiR2119) rendered soybean more susceptible to SCN. Conversely, silencing miR2119 (STTM2119) increased soybean resistance against SCN. Furthermore, RNA-seq analysis revealed that miR2119 is involved in many defense signaling pathways. GUS reporter gene assays demonstrated that miR2119 targets GmADH1.1a and GmADH1.1b . Functional analysis indicated that ADHs act as a major role in responding to H. glycines by modulating reactive oxygen species (ROS) levels. Together, the findings reveal a novel mechanism by which the polycistronic precursor miR2119–398a coordinately regulates in response to H. glycines . Additionally, miR2119 becomes an essential element contributing to H. glycines by modulating ADH activity and ROS homeostasis in soybean.
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Claims 11
Claim · model-written summary
Nematode infection significantly reduced transcript levels of precursors containing miR2119 and miR398a.
- Taxon
- soybean
Glycine maxe0 - Gene
- miR2119 and miR398ae0
- Outcome
- transcript levelse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript levels of polycistronic precursors containing miR2119 and miR398a were significantly reduced in soybean upon nematode infection.”
Claim · model-written summary
Soybean miR2119-398a promoter activity decreased during SCN infection.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- SCNe0pathogen
- Gene
- miR2119-398ae0
- Outcome
- miR2119-398a promotere0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS activity assays demonstrated a decrease in miR2119-398a promoter during SCN infection.”
t0“Soybean”
Claim · model-written summary
Overexpression of the miR2119-398a precursor increased soybean susceptibility to SCN.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Overexpression of polycistronic precursor miR2119-398a (OE-premiR2119-398a)e0
- Interactor
- SCNe0pathogen
- Outcome
- susceptible to SCNe0
- Direction
- more susceptiblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of polycistronic precursor miR2119-398a (OE-premiR2119-398a) and miR2119 precursor (OE-premiR2119) rendered soybean more susceptible to SCN.”
Claim · model-written summary
Overexpression of the miR2119 precursor increased soybean susceptibility to SCN.
- Taxon
- soybeanGlycine maxe0
- Treatment
- miR2119 precursor (OE-premiR2119)e0
- Interactor
- SCNe0pathogen
- Outcome
- susceptible to SCNe0
- Direction
- more susceptiblee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of polycistronic precursor miR2119-398a (OE-premiR2119-398a) and miR2119 precursor (OE-premiR2119) rendered soybean more susceptible to SCN.”
Claim · model-written summary
Silencing miR2119 increased soybean resistance against SCN.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- silencing miR2119 (STTM2119)e0
- Interactor
- SCNe0pathogen
- Outcome
- soybean resistance against SCNe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing miR2119 (STTM2119) increased soybean resistance against SCN.”
t0“Soybean”
Claim · model-written summary
RNA-seq implicated miR2119 in multiple defense signaling pathways.
- Taxon
- SoybeanGlycine maxt0
- Gene
- miR2119e0
- Outcome
- defense signaling pathwayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR2119 is involved in many defense signaling pathways.”
t0“Soybean”
Claim · model-written summary
GUS reporter assays showed miR2119 targets GmADH1.1a.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmADH1.1ae0
- Outcome
- miR2119 targets GmADH1.1ae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR2119 targets GmADH1.1a and GmADH1.1b”
t0“Soybean”
Claim · model-written summary
GUS reporter assays showed miR2119 targets GmADH1.1b.
- Taxon
- SoybeanGlycine maxt0
- Gene
- GmADH1.1be0
- Outcome
- miR2119 targets GmADH1.1a and GmADH1.1be0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR2119 targets GmADH1.1a and GmADH1.1b”
t0“Soybean”
Claim · model-written summary
ADHs modulate reactive oxygen species levels in the response to H. glycines.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- H. glycines
Heterodera glycinese0pathogen - Gene
- ADHse0
- Outcome
- reactive oxygen species (ROS) levelse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ADHs act as a major role in responding to H. glycines by modulating reactive oxygen species (ROS) levels.”
t0“Soybean”
Claim · model-written summary
The precursor miR2119–398a coordinately regulates the response to H. glycines.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- H. glycinesHeterodera glycinese0pathogen
- Gene
- miR2119–398ae0
- Outcome
- response to H. glycinese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polycistronic precursor miR2119–398a coordinately regulates in response to H. glycines”
t0“Soybean”
Claim · model-written summary
miR2119 contributes to the soybean response to H. glycines by modulating ADH activity and ROS homeostasis.
- Taxon
- soybeanGlycine maxe0
- Interactor
- H. glycinesHeterodera glycinese0pathogen
- Gene
- miR2119e0
- Outcome
- ADH activity and ROS homeostasise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR2119 becomes an essential element contributing to H. glycines by modulating ADH activity and ROS homeostasis in soybean.”
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