Abstract
Improving seed germination and seedling development can potentially increase crop yield and improve quality in direct-seeded rice. This study aimed to detect loci or genes associated with rice seed germination. We reported the phenotypic analysis of seed germination in 103 rice accessions across two years, and a genome-wide association study (GWAS) was conducted to identify loci underlying the genetic regulation of seed germination. A total of seven genetic loci were found to be associated with seed germination, including five loci that overlapped with the previously reported loci/genes, and two novel loci. Of these, two loci (qGP2 and qGP4.1) were stable across different environments. GP4 (Germination percentage 4), encoding a 9-cis-epoxycarotenoid dioxygenase, was identified as the candidate gene of the major locus qGP4.1. A sequence analysis of GP4 revealed that four functional polymorphic sites in the coding region were significantly associated with germination percentage. The disruption of GP4 by gene editing resulted in faster seed germination and seedling establishment. Taken together, we have identified GP4 as a novel gene involved in rice seed germination, and we provide a potential target gene for improving rice seed vigor via gene editing or molecular breeding.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Seven genetic loci were associated with rice seed germination.
- Taxon
- Rice
Oryza sativat0 - Gene
- genetic locie0
- Outcome
- seed germinatione0
- Value
- sevene0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of seven genetic loci were found to be associated with seed germination”
t0“Rice”
Claim · model-written summary
Five of the associated loci overlapped previously reported loci or genes.
- Taxon
- RiceOryza sativat0
- Outcome
- loci that overlapped with the previously reported loci/genese0
- Value
- five locie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including five loci that overlapped with the previously reported loci/genes”
t0“Rice”
Claim · model-written summary
Two germination loci were novel.
- Taxon
- RiceOryza sativat0
- Outcome
- novel locie0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and two novel loci”
t0“Rice”
Claim · model-written summary
qGP2 and qGP4.1 were stable across environments.
- Taxon
- RiceOryza sativat0
- Gene
- qGP2 and qGP4.1e0
- Outcome
- stable across different environmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two loci (qGP2 and qGP4.1) were stable across different environments”
t0“Rice”
Claim · model-written summary
GP4, encoding a dioxygenase, was identified as candidate for qGP4.1.
- Taxon
- RiceOryza sativat0
- Gene
- GP4e0
- Outcome
- candidate gene of the major locus qGP4.1e0
- Qualifier
- encoding a 9-cis-epoxycarotenoid dioxygenasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GP4 (Germination percentage 4), encoding a 9-cis-epoxycarotenoid dioxygenase, was identified as the candidate gene of the major locus qGP4.1”
t0“Rice”
Claim · model-written summary
Four GP4 coding polymorphisms were significantly associated with germination percentage.
- Taxon
- RiceOryza sativat0
- Gene
- GP4e0
- Outcome
- germination percentagee0
- Value
- foure0
- Qualifier
- functional polymorphic sites in the coding regione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GP4 (Germination percentage 4), encoding a 9-cis-epoxycarotenoid dioxygenase, was identified as the candidate gene of the major locus qGP4.1. A sequence analysis of GP4 revealed that four functional polymorphic sites in the coding region were significantly associated with germination percentage.”
t0“Rice”
Claim · model-written summary
Disrupting GP4 by editing led to faster seed germination.
- Taxon
- RiceOryza sativat0
- Treatment
- disruption of GP4 by gene editinge0
- Gene
- GP4e0
- Outcome
- seed germinatione0
- Direction
- fastere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disruption of GP4 by gene editing resulted in faster seed germination and seedling establishment”
t0“Rice”
Claim · model-written summary
Disrupting GP4 by editing led to faster seedling establishment.
- Taxon
- RiceOryza sativat0
- Treatment
- disruption of GP4 by gene editinge0
- Gene
- GP4e0
- Outcome
- seedling establishmente0
- Direction
- fastere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disruption of GP4 by gene editing resulted in faster seed germination and seedling establishment”
t0“Rice”
Claim · model-written summary
GP4 is proposed as a target to improve seed vigor by editing or breeding.
- Taxon
- riceOryza sativae0
- Gene
- GP4t0
- Outcome
- improving rice seed vigor via gene editing or molecular breedinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we provide a potential target gene for improving rice seed vigor via gene editing or molecular breeding”
t0“identified GP4 as a novel gene”
The same questions in other papers
loss of function · growth · Oryza sativa (rice)Positive and negative results
27 papers in the same direction as this paper · 104 in the opposite direction
- negative
- negative
- negative
128 papers more in this group
See all 132 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.