Abstract
Powdery mildew and stripe rust are major diseases on wheat worldwide that cause significant reductions in wheat production. The ribosomal silencing factor (RsfS) has been proven to regulate protein biosynthesis by inhibiting the translation process in bacterial response to stress. However, the role of RsfS in plant resistance to biotic stresses remains unclear. In this study, the RsfS homolog, TaRsfS was isolated from wheat. Overexpression of TaRsfS (TaRsfS-OE) reduces wheat resistance to powdery mildew and stripe rust and TaRsfS knockout (TaRsfS-KO) increases wheat resistance to both diseases without affecting key agronomic traits. The interaction protein of TaRsfS, 12-oxo-phytodienoic acid reductase 1 (TaOPR1), a key enzyme in the biosynthesis of jasmonic acid (JA), was screened and identified. Knocking-down and overexpression of TaOPR1 indicated that TaOPR1 positively regulates wheat resistance to powdery mildew and stripe rust. TaRsfS may regulate TaOPR1 at upstream, bind to the enzyme activity pocket of TaOPR1 and affect TaOPR1 enzyme activity, resulting in a reduced JA biosynthesis and wheat susceptible to powdery mildew and stripe rust. Collectively, TaRsfS is a susceptibility gene and negatively regulates wheat resistance to powdery mildew and stripe rust, and it has good potential for improving wheat resistance by genetic modifications.
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Claims 10
Claim · model-written summary
Overexpression of TaRsfS reduced wheat resistance to powdery mildew
- Taxon
- wheat
Triticum aestivume1 - Treatment
- Overexpression of TaRsfSe1
- Interactor
- powdery mildewe1pathogen
- Gene
- TaRsfSe1
- Outcome
- resistancee1
- Direction
- reducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of TaRsfS (TaRsfS-OE) reduces wheat resistance to powdery mildew and stripe rust”
Claim · model-written summary
Overexpression of TaRsfS reduced wheat resistance to stripe rust
- Taxon
- wheatTriticum aestivume1
- Treatment
- Overexpression of TaRsfSe1
- Interactor
- stripe ruste1pathogen
- Gene
- TaRsfSe1
- Outcome
- resistancee1
- Direction
- reducese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of TaRsfS (TaRsfS-OE) reduces wheat resistance to powdery mildew and stripe rust”
Claim · model-written summary
Knockout of TaRsfS increased wheat resistance to powdery mildew
- Taxon
- wheatTriticum aestivume2
- Treatment
- TaRsfS knockoute2
- Interactor
- powdery mildewe1pathogen
- Gene
- TaRsfSe2
- Outcome
- resistancee2
- Direction
- increasese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of TaRsfS (TaRsfS-OE) reduces wheat resistance to powdery mildew and stripe rust”
e2“TaRsfS knockout (TaRsfS-KO) increases wheat resistance to both diseases without affecting key agronomic traits”
Claim · model-written summary
Knockout of TaRsfS increased wheat resistance to stripe rust
- Taxon
- wheatTriticum aestivume2
- Treatment
- TaRsfS knockoute2
- Interactor
- stripe ruste1pathogen
- Gene
- TaRsfSe2
- Outcome
- resistancee2
- Direction
- increasese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Overexpression of TaRsfS (TaRsfS-OE) reduces wheat resistance to powdery mildew and stripe rust”
e2“TaRsfS knockout (TaRsfS-KO) increases wheat resistance to both diseases without affecting key agronomic traits”
Claim · model-written summary
Knockout of TaRsfS did not affect key agronomic traits of wheat
- Taxon
- wheatTriticum aestivume2
- Treatment
- TaRsfS knockoute2
- Gene
- TaRsfSe2
- Outcome
- key agronomic traitse2
- Direction
- without affectinge2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“TaRsfS knockout (TaRsfS-KO) increases wheat resistance to both diseases without affecting key agronomic traits”
Claim · model-written summary
TaOPR1 was identified as an interaction protein of TaRsfS in wheat
- Taxon
- wheatTriticum aestivume0
- Gene
- TaOPR1e1
- Outcome
- interaction protein of TaRsfSe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Powdery mildew and stripe rust are major diseases on wheat worldwide”
e1“The interaction protein of TaRsfS, 12-oxo-phytodienoic acid reductase 1 (TaOPR1), a key enzyme in the biosynthesis of jasmonic acid (JA), was screened and identified”
Claim · model-written summary
Knocking down and overexpressing TaOPR1 positively regulated wheat resistance to powdery mildew
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knocking-down and overexpression of TaOPR1e1
- Interactor
- powdery mildewe1pathogen
- Gene
- TaOPR1e1
- Outcome
- resistancee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Knocking-down and overexpression of TaOPR1 indicated that TaOPR1 positively regulates wheat resistance to powdery mildew and stripe rust”
Claim · model-written summary
Knocking down and overexpressing TaOPR1 positively regulated wheat resistance to stripe rust
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knocking-down and overexpression of TaOPR1e1
- Interactor
- stripe ruste1pathogen
- Gene
- TaOPR1e1
- Outcome
- resistancee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Knocking-down and overexpression of TaOPR1 indicated that TaOPR1 positively regulates wheat resistance to powdery mildew and stripe rust”
Claim · model-written summary
TaRsfS probably acts upstream of TaOPR1, reducing jasmonic acid biosynthesis and wheat susceptibility
- Taxon
- wheatTriticum aestivume1
- Gene
- TaRsfSe1
- Outcome
- JA biosynthesise1
- Qualifier
- affect TaOPR1 enzyme activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaRsfS may regulate TaOPR1 at upstream, bind to the enzyme activity pocket of TaOPR1 and affect TaOPR1 enzyme activity, resulting in a reduced JA biosynthesis and wheat susceptible to powdery mildew and stripe rust”
Claim · model-written summary
TaRsfS has good potential for improving wheat resistance by genetic modification
- Taxon
- wheatTriticum aestivume1
- Outcome
- improving wheat resistance by genetic modificationse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“it has good potential for improving wheat resistance by genetic modifications”