Abstract
Waterlogging is expected to become a more prominent yield restricting stress for barley as rainfall frequency is increasing in many regions due to climate change. The duration of waterlogging events in the field is highly variable throughout the season, and this variation is also observed in experimental waterlogging studies. Such variety of protocols make intricate physiological responses challenging to assess and quantify. To assess barley waterlogging tolerance in controlled conditions, we present an optimal duration and setup of simulated waterlogging stress using image-based phenotyping. Six protocols durations, 5, 10, and 14 days of stress with and without seven days of recovery, were tested. To quantify the physiological effects of waterlogging on growth and greenness, we used top down and side view RGB (Red-Green-Blue) images. These images were taken daily throughout each of the protocols using the PSI PlantScreen™ imaging platform. Two genotypes of two-row spring barley, grown in glasshouse conditions, were subjected to each of the six protocols, with stress being imposed at the three-leaf stage. Shoot biomass and root imaging data were analysed to determine the optimal stress protocol duration, as well as to quantify the growth and morphometric changes of barley in response to waterlogging stress. Our time-series results show a significant growth reduction and alteration of greenness, allowing us to determine an optimal protocol duration of 14 days of stress and seven days of recovery for controlled conditions. Moreover, to confirm the reproducibility of this protocol, we conducted the same experiment in a different facility equipped with RGB and chlorophyll fluorescence imaging sensors. Our results demonstrate that the selected protocol enables the assessment of genotypic differences, which allow us to further determine tolerance responses in a glasshouse environment. Altogether, this work presents a new and reproducible image-based protocol to assess early stage waterlogging tolerance, empowering a precise quantification of waterlogging stress relevant markers such as greenness, Fv/Fm and growth rates.
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Claims 5
Claim · model-written summary
Waterlogging significantly reduced barley growth.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- stresse0
- Outcome
- growthe0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Our time-series results show a significant growth reduction and alteration of greenness, allowing us to determine an optimal protocol duration of 14 days of stress and seven days of recovery for controlled conditions.”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Waterlogging altered barley greenness.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- stresse0
- Outcome
- greennesse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our time-series results show a significant growth reduction and alteration of greenness, allowing us to determine an optimal protocol duration of 14 days of stress and seven days of recovery for controlled conditions.”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Fourteen days of stress and seven days of recovery was the optimal controlled-condition protocol.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- optimal protocol duratione0
- Value
- 14 days of stress and seven days of recoverye0
- Setting
- controlled conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our time-series results show a significant growth reduction and alteration of greenness, allowing us to determine an optimal protocol duration of 14 days of stress and seven days of recovery for controlled conditions.”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
The selected protocol detected barley genotypic differences in a glasshouse.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- assessment of genotypic differencese0
- Setting
- glasshouse environmente0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results demonstrate that the selected protocol enables the assessment of genotypic differences, which allow us to further determine tolerance responses in a glasshouse environment.”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
The image-based protocol reproducibly quantified greenness, Fv/Fm and growth rates as waterlogging markers.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- greenness, Fv/Fm and growth ratese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this work presents a new and reproducible image-based protocol to assess early stage waterlogging tolerance, empowering a precise quantification of waterlogging stress relevant markers such as greenness, Fv/Fm and growth rates.”
t0“barley (Hordeum vulgare L.)”