Abstract
Cryopreservation of plant material of valuable plants is an effective way to preserve biological diversity in conditions of active and widespread anthropogenic pressure on natural ecosystems. Introduction to cryocollections is justified not only for food agricultural plants, but also for medicinal plants that have valuable secondary metabolites and essential oils. These include Dracocephalum ruyschian and Salvia sclarea. To effectively restore the viability of seeds after freezing in liquid nitrogen, we examined the influence of various cryoprotectants: glycerin, dimethyl sulfoxide and polyvinypyrrolidone, as well as various methods of their application — at room temperature and in the cold. It was found that for effective cryopreservation of Dracocephalum ruyschiana seed material, the use of cryoprotectors is justified; however, the method of their application does not matter. When cryopreserving Salvia sclarea seed material, it is important to use cryoprotectors and introduce them into the cooling system, preferably at the crystallization temperature of water; in this case, the energy of seed germination increases significantly.
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Claims 4
Claim · model-written summary
Cryoprotectors were justified for effective cryopreservation of Dracocephalum ruyschiana seed material.
- Taxon
- Dracocephalum ruyschiana
Dracocephalum ruyschianae0 - Treatment
- cryoprotectorse0
- Outcome
- effective cryopreservatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“for effective cryopreservation of Dracocephalum ruyschiana seed material, the use of cryoprotectors is justified”
Claim · model-written summary
The method of cryoprotector application did not matter for Dracocephalum ruyschiana seed cryopreservation.
- Taxon
- Dracocephalum ruyschianaDracocephalum ruyschianae0
- Treatment
- method of their applicatione0
- Outcome
- effective cryopreservatione0
- Direction
- does not mattere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“for effective cryopreservation of Dracocephalum ruyschiana seed material, the use of cryoprotectors is justified; however, the method of their application does not matter”
Claim · model-written summary
Cryoprotectors were important for cryopreserving Salvia sclarea seed material.
- Taxon
- Salvia sclarea
Salvia sclareae0 - Treatment
- cryoprotectorse0
- Outcome
- cryopreserving Salvia sclarea seed materiale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When cryopreserving Salvia sclarea seed material, it is important to use cryoprotectors”
Claim · model-written summary
Introducing cryoprotectors into the cooling system preferably at the crystallization temperature of water significantly increased Salvia sclarea seed germination energy.
- Taxon
- Salvia sclareaSalvia sclareae0
- Treatment
- introduce them into the cooling systeme0
- Outcome
- energy of seed germinatione0
- Direction
- increasese0
- Qualifier
- at the crystallization temperature of watere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When cryopreserving Salvia sclarea seed material, it is important to use cryoprotectors and introduce them into the cooling system, preferably at the crystallization temperature of water; in this case, the energy of seed germination increases significantly”