Abstract
Mulberry crinkle leaf virus (MCLV), identified in mulberry plants (Morus alba L.), is a member of the genus Mulcrilevirus in the family Geminiviridae. The functions of the V2 protein encoded by MCLV remain unclear. Here, Agrobacterium-mediated infectious clones of a wild-type MCLV vII (MCLVWT) and two V2 mutant MCLV vIIs, including MCLVmV2 (with a mutation of the start codon of the V2 ORF) and MCLVdV2 (5′-end partial deletion of the V2 ORF sequence), were constructed to investigate the roles of V2 both in planta and at the cellular level. Although all three constructs (pCA-1.1MCLVWT, pCA-MCLVmV2, and pCA-MCLVdV2) were able to infect both natural host mulberry plants and experimental tomato plants systematically, the replication of the MCLVmV2 and MCLVdV2 genomes in these hosts was significantly reduced compared to that of MCLVWT. Similarly, the accumulation of MCLVmV2 and MCLVdV2 in protoplasts of Nicotiana benthamiana plants was significantly lower than that of MCLVWT either 24 h or 48 h post-transfection. A complementation experiment further confirmed that the decreased accumulation of MCLV in the protoplasts was due to the absence of V2 expression. These results revealed that MCLV-encoded V2 greatly enhances the level of MCLV DNA accumulation and is designated the replication enhancer protein of MCLV.
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Claims 5
Claim · model-written summary
MCLVmV2 and MCLVdV2 V2 mutants show significantly reduced genome replication in mulberry and tomato compared with wild-type MCLV.
Verbatim
e0“the replication of the MCLVmV2 and MCLVdV2 genomes in these hosts was significantly reduced compared to that of MCLVWT”
e1“were able to infect both natural host mulberry plants and experimental tomato plants systematically”
Claim · model-written summary
MCLVmV2 and MCLVdV2 accumulate to significantly lower levels than MCLVWT in N. benthamiana protoplasts at 24 h and 48 h after transfection.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- protoplastse0
- Treatment
- MCLVmV2 and MCLVdV2e0
- Interactor
- MCLVMulberry crinkle leaf viruse0pathogen
- Outcome
- accumulation of MCLVmV2 and MCLVdV2e0
- Direction
- significantly lowere0
- Comparator
- MCLVWTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of MCLVmV2 and MCLVdV2 in protoplasts of Nicotiana benthamiana plants was significantly lower than that of MCLVWT either 24 h or 48 h post-transfection”
Claim · model-written summary
Complementation confirms that the decreased MCLV accumulation in protoplasts is due to the absence of V2 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Interactor
- MCLVMulberry crinkle leaf viruse1pathogen
- Gene
- V2e1
- Outcome
- decreased accumulation of MCLV in the protoplastse1
- Qualifier
- complementation experimente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the accumulation of MCLVmV2 and MCLVdV2 in protoplasts of Nicotiana benthamiana plants was significantly lower than that of MCLVWT either 24 h or 48 h post-transfection”
e1“A complementation experiment further confirmed that the decreased accumulation of MCLV in the protoplasts was due to the absence of V2 expression”
Claim · model-written summary
MCLV-encoded V2 greatly enhances the level of MCLV DNA accumulation and is designated the replication enhancer protein.
- Taxon
- mulberry plantse0
- Interactor
- MCLVMulberry crinkle leaf viruse1pathogen
- Gene
- MCLV-encoded V2e1
- Outcome
- level of MCLV DNA accumulatione1
Verbatim
e0“identified in mulberry plants (Morus alba L.)”
e1“MCLV-encoded V2 greatly enhances the level of MCLV DNA accumulation”
Claim · model-written summary
All three MCLV constructs (wild type and two V2 mutants) infect natural host mulberry plants and experimental tomato plants systemically.
Verbatim
e0“were able to infect both natural host mulberry plants and experimental tomato plants systematically”