Paper · 2024 · South Eastern European Journal of Public Health

Effect Of Cytokinin BA And Different Concentrations Of Chemical Stress Treatments On The Multiplication Of Vegetative Shoots Of The Plant Antirrhinum Magus L. Grown In Vitro

Lamyaa Hussein Mousa Mazene; Shaymaa Hussein Mousa Mazeni

15
claims
31
verbatim quotes
1
citations
3
research areas
✓
Open access · cc-by-nd

Abstract

The study was conducted on the multiplication of Antirrhinum magus L. plant using the micropropagation technique, represented by culture vegetative shoots on MS nutrient medium prepared with different concentrations of cytokinin BA and different levels of chemical stress treatments. The treatment of 4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1 gave the highest significant increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per plantlet, and leaf width, which amounted to 6.40 shoot explant-1, 4.00 cm, 20.60 shoot leaves -1, 0.68 cm, respectively, while a significant decrease in the average vegetative traits was observed when the concentration of BA in the medium was increased in the two treatments (8.88, 11.10) μmol.L–1 BA, which did not differ significantly between them. The results of chemical stress showed that reducing the level Mannitol to 15gm L-1 in multiplication medium led to a significant and inverse increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per shoot, and leaf width reached 4.80 shoot explant-1, 3.50 cm, 11.40 shoot explant1-, 0.44 cm in succession, and their averages decreased. By increasing the concentration in the middle. It was also observed that there was an inverse relationship between the average vegetative traits and the levels of PEG8000, where the control treatment achieved the highest significant increase in the average of all vegetative traits, amounting to 6.60 shoot explant-1, 4.06 cm, and 7.60 shoot explant-1, 0.92 cm, respectively, compared to the other averages of water stress whenever the level of PEG8000 increased. In the nutrient medium down to the level of 45g. L-1.

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Claims 15

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BA with NAA gave the highest significant average shoot number, 6.40 shoot explant-1.

Taxon
Antirrhinum magus L.e1
Treatment
4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1e0
Outcome
average number of vegetative shoots per plantlete0
Direction
highest significant increasee0
Value
6.40 shoot explant-1e0

Not reportedComparator

Verbatim

e0

“The treatment of 4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1 gave the highest significant increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per plantlet, and leaf width, which amounted to 6.40 shoot explant-1, 4.00 cm, 20.60 shoot leaves -1, 0.68 cm, respectively”

✓ located verbatim in the source · abstract, characters 284–609 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BA with NAA gave the highest significant shoot length, 4.00 cm.

Taxon
Antirrhinum magus L.e1
Treatment
4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1e0
Outcome
shoot lengthe0
Direction
highest significant increasee0
Value
4.00 cme0

Not reportedComparator

Verbatim

e0

“The treatment of 4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1 gave the highest significant increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per plantlet, and leaf width, which amounted to 6.40 shoot explant-1, 4.00 cm, 20.60 shoot leaves -1, 0.68 cm, respectively”

✓ located verbatim in the source · abstract, characters 284–609 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BA with NAA gave the highest significant leaf number, 20.60 shoot leaves -1.

Taxon
Antirrhinum magus L.e1
Treatment
4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1e0
Outcome
number of leaves per plantlete0
Direction
highest significant increasee0
Value
20.60 shoot leaves -1e0

Not reportedComparator

Verbatim

e0

“The treatment of 4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1 gave the highest significant increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per plantlet, and leaf width, which amounted to 6.40 shoot explant-1, 4.00 cm, 20.60 shoot leaves -1, 0.68 cm, respectively”

✓ located verbatim in the source · abstract, characters 284–609 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

BA with NAA gave the highest significant leaf width, 0.68 cm.

Taxon
Antirrhinum magus L.e1
Treatment
4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1e0
Outcome
leaf widthe0
Direction
highest significant increasee0
Value
0.68 cme0

Not reportedComparator

Verbatim

e0

“The treatment of 4.44 μmol.L–1 BA with a NAA concentration of 0.54 μmol.L–1 gave the highest significant increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per plantlet, and leaf width, which amounted to 6.40 shoot explant-1, 4.00 cm, 20.60 shoot leaves -1, 0.68 cm, respectively”

✓ located verbatim in the source · abstract, characters 284–609 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Higher BA concentrations significantly decreased vegetative traits without a significant difference between the two doses.

Taxon
Antirrhinum magus L.e1
Treatment
(8.88, 11.10) μmol.L–1 BAe0
Outcome
average vegetative traitse0
Direction
decreasee0
Qualifier
did not differ significantly between theme0

Not reportedComparator

Verbatim

e0

“a significant decrease in the average vegetative traits was observed when the concentration of BA in the medium was increased in the two treatments (8.88, 11.10) μmol.L–1 BA, which did not differ significantly between them”

✓ located verbatim in the source · abstract, characters 617–839 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

At 15gm L-1 mannitol, shoot number significantly increased to 4.80 shoot explant-1.

Taxon
Antirrhinum magus L.e1
Treatment
Mannitol to 15gm L-1e0
Outcome
average number of vegetative shoots per plantlete0
Direction
increasee0
Value
4.80 shoot explant-1e0

Not reportedComparator

Verbatim

e0

“reducing the level Mannitol to 15gm L-1 in multiplication medium led to a significant and inverse increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per shoot, and leaf width reached 4.80 shoot explant-1, 3.50 cm, 11.40 shoot explant1-, 0.44 cm in succession”

✓ located verbatim in the source · abstract, characters 884–1188 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

At 15gm L-1 mannitol, shoot length increased to 3.50 cm.

Taxon
Antirrhinum magus L.e1
Treatment
Mannitol to 15gm L-1e0
Outcome
shoot lengthe0
Direction
increasee0
Value
3.50 cme0

Not reportedComparator

Verbatim

e0

“reducing the level Mannitol to 15gm L-1 in multiplication medium led to a significant and inverse increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per shoot, and leaf width reached 4.80 shoot explant-1, 3.50 cm, 11.40 shoot explant1-, 0.44 cm in succession”

✓ located verbatim in the source · abstract, characters 884–1188 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

At 15gm L-1 mannitol, leaf number increased to 11.40 shoot explant1-.

Taxon
Antirrhinum magus L.e1
Treatment
Mannitol to 15gm L-1e0
Outcome
number of leaves per shoote0
Direction
increasee0
Value
11.40 shoot explant1-e0

Not reportedComparator

Verbatim

e0

“reducing the level Mannitol to 15gm L-1 in multiplication medium led to a significant and inverse increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per shoot, and leaf width reached 4.80 shoot explant-1, 3.50 cm, 11.40 shoot explant1-, 0.44 cm in succession”

✓ located verbatim in the source · abstract, characters 884–1188 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

At 15gm L-1 mannitol, leaf width increased to 0.44 cm.

Taxon
Antirrhinum magus L.e1
Treatment
Mannitol to 15gm L-1e0
Outcome
leaf widthe0
Direction
increasee0
Value
0.44 cme0

Not reportedComparator

Verbatim

e0

“reducing the level Mannitol to 15gm L-1 in multiplication medium led to a significant and inverse increase in the average number of vegetative shoots per plantlet, shoot length, number of leaves per shoot, and leaf width reached 4.80 shoot explant-1, 3.50 cm, 11.40 shoot explant1-, 0.44 cm in succession”

✓ located verbatim in the source · abstract, characters 884–1188 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Increasing mannitol concentration decreased average vegetative traits.

Taxon
Antirrhinum magus L.e2
Treatment
Mannitole1
Outcome
averagese0
Direction
decreasede0

Verbatim

e0

“their averages decreased. By increasing the concentration in the middle”

✓ located verbatim in the source · abstract, characters 1194–1265 · show in text ↑
e1

“The results of chemical stress showed that reducing the level Mannitol to 15gm L-1”

✓ located verbatim in the source · abstract, characters 841–923 · show in text ↑
e2

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

With PEG8000, control shoot number was highest at 6.60 shoot explant-1.

Taxon
Antirrhinum magus L.e1
Treatment
control treatmente0
Outcome
vegetative traitse0
Direction
highest significant increasee0
Value
6.60 shoot explant-1e0
Comparator
other averages of water stresse0

Verbatim

e0

“the control treatment achieved the highest significant increase in the average of all vegetative traits, amounting to 6.60 shoot explant-1, 4.06 cm, and 7.60 shoot explant-1, 0.92 cm, respectively, compared to the other averages of water stress whenever the level of PEG8000 increased”

✓ located verbatim in the source · abstract, characters 1398–1682 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

With PEG8000, control shoot length was highest at 4.06 cm.

Taxon
Antirrhinum magus L.e1
Treatment
control treatmente0
Outcome
vegetative traitse0
Direction
highest significant increasee0
Value
4.06 cme0
Comparator
other averages of water stresse0

Verbatim

e0

“the control treatment achieved the highest significant increase in the average of all vegetative traits, amounting to 6.60 shoot explant-1, 4.06 cm, and 7.60 shoot explant-1, 0.92 cm, respectively, compared to the other averages of water stress whenever the level of PEG8000 increased”

✓ located verbatim in the source · abstract, characters 1398–1682 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

With PEG8000, control leaf number was highest at 7.60 shoot explant-1.

Taxon
Antirrhinum magus L.e1
Treatment
control treatmente0
Outcome
vegetative traitse0
Direction
highest significant increasee0
Value
7.60 shoot explant-1e0
Comparator
other averages of water stresse0

Verbatim

e0

“the control treatment achieved the highest significant increase in the average of all vegetative traits, amounting to 6.60 shoot explant-1, 4.06 cm, and 7.60 shoot explant-1, 0.92 cm, respectively, compared to the other averages of water stress whenever the level of PEG8000 increased”

✓ located verbatim in the source · abstract, characters 1398–1682 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

With PEG8000, control leaf width was highest at 0.92 cm.

Taxon
Antirrhinum magus L.e1
Treatment
control treatmente0
Outcome
vegetative traitse0
Direction
highest significant increasee0
Value
0.92 cme0
Comparator
other averages of water stresse0

Verbatim

e0

“the control treatment achieved the highest significant increase in the average of all vegetative traits, amounting to 6.60 shoot explant-1, 4.06 cm, and 7.60 shoot explant-1, 0.92 cm, respectively, compared to the other averages of water stress whenever the level of PEG8000 increased”

✓ located verbatim in the source · abstract, characters 1398–1682 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Average vegetative traits declined as PEG8000 level increased.

Taxon
Antirrhinum magus L.e1
Treatment
PEG8000e0
Outcome
average vegetative traitse0

Not reportedDirection

Verbatim

e0

“there was an inverse relationship between the average vegetative traits and the levels of PEG8000”

✓ located verbatim in the source · abstract, characters 1293–1390 · show in text ↑
e1

“Antirrhinum magus L. plant”

✓ located verbatim in the source · abstract, characters 49–75 · show in text ↑