Abstract
Aim: The aim of this research was to investigate the effects of different plant growth regulators and MS culture medium on the characteristics of callus weight, percentage of callus formation and embryogenesis in anther culture of two cultivars of Sina and Negin cucumbers (Cucumis sativus L.).Materials and methods: The experiment was conducted as a factorial in a completely randomized design with three replications. In this research, to determine the stage of growth and development of microspores, male flower buds in the sizes of 0.5 to 2.2 cm were taken from mother plants. For dyeing, the anthers were separated from the buds and placed in stocarman dye solution containing one gram of carmen powder and one hundred milliliters of 45% acetic acid for one day. Then the anthers were crushed on the laboratory slide and the microspores inside them were observed under a light microscope. In the callus induction test, the factors include two cucumber cultivars (Sina and Negin), the combination of different concentrations of BAP (0, 0.7, 0.9 and 1.1 mg/liter) and 2,4-D (0, 0.75, 1 and 1.25 mg/liter). 4 anthers were cultured in each petri dish. After placing the anthers in the culture medium, the lids of the petri dishes were closed and sealed with parafilm, and then they were covered with aluminum foil and placed in the growth chamber at a temperature of 25 ± 2 degrees Celsius in the dark for 2 weeks and the environments They were cultivated once every 2 or 3 weeks.Results: Cytogenetic experiments showed that 1.5 cm buds containing anthers and microspores in the mononuclear stage are suitable for anther culture. The results of the analysis of the variance of the data showed a statistically significant difference at the level of 0.01 between the interaction effects of genotype and culture medium. Anthers cultured in MS base medium and media prepared with different concentrations of BAP did not show a response and were destroyed after one month of cultivation. The average main effect of cultivars on callus fresh weight after several weeks of cultivation showed that the highest callus fresh weight was obtained in Sina variety. The comparison of the average triple interaction effects of cultivar × BAP × 2,4-D for callus fresh weight in this research has shown that Sina cultivar in MS culture medium contains the growth regulator compound 0.7 mg/liter BAP and 1 mg/liter 2,4 -D has the highest callus fresh weight. By examining the interaction effect of cultivar, auxin and cytokinin, it was found that the highest percentage of callus formation was 100% in both cultivars in environments with the combination of BAP (0.7 and 0.9 mg/liter) with 2,4-D (0.75, 1 and 1.25 mg/L) and in Sina cultivar BAP (1.1 mg/L) with 2,4-D (0.75, 1.1 and 1.25 mg/L). However, the lowest percentage of callus formation was observed in Nagin cultivar in the presence of 2,4-D hormone with a concentration of 1 mg/liter with an average of 33.333%.Conclusion: In general, the use of growth regulator BAP and 2,4-D in MS culture medium induced callus in anther culture of both cucumber cultivars.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Buds of 1.5 cm with mononuclear microspores were suitable for cucumber anther culture.
- Taxon
- cucumber
Cucumis sativust0 - Organ / stage
- anthers and microspores in the mononuclear stagee0
- Outcome
- suitable for anther culturee0
- Value
- 1.5 cme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1.5 cm buds containing anthers and microspores in the mononuclear stage are suitable for anther culture”
t0“cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Genotype and culture medium had a significant interaction effect on the studied response.
- Taxon
- cucumberCucumis sativust0
- Material
- genotypee0
- Outcome
- interaction effects of genotype and culture mediume0
- Value
- 0.01e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“statistically significant difference at the level of 0.01 between the interaction effects of genotype and culture medium”
t0“cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Anthers in MS base medium showed no response and were destroyed after one month.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- Antherse0
- Treatment
- MS base mediume0
- Outcome
- did not show a response and were destroyede0
- Qualifier
- after one month of cultivatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Anthers cultured in MS base medium and media prepared with different concentrations of BAP did not show a response and were destroyed after one month of cultivation”
t0“cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Anthers in BAP media showed no response and were destroyed after one month.
- Taxon
- cucumberCucumis sativust0
- Organ / stage
- antherst0
- Treatment
- different concentrations of BAPe0
- Outcome
- did not show a response and were destroyede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“media prepared with different concentrations of BAP did not show a response and were destroyed after one month of cultivation”
t0“anthers of cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Sina had the highest callus fresh weight among the cultivars.
- Taxon
- cucumberCucumis sativust0
- Material
- Sinae0
- Outcome
- callus fresh weighte0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest callus fresh weight was obtained in Sina variety”
t0“cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Sina in MS medium with 0.7 mg/liter BAP and 1 mg/liter 2,4-D had the highest callus fresh weight.
- Taxon
- cucumberCucumis sativust0
- Material
- Sinae0
- Treatment
- 0.7 mg/liter BAP and 1 mg/liter 2,4 -De0
- Outcome
- callus fresh weighte0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sina cultivar in MS culture medium contains the growth regulator compound 0.7 mg/liter BAP and 1 mg/liter 2,4 -D has the highest callus fresh weight”
t0“cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Both cultivars reached 100% callus formation under specified BAP and 2,4-D combinations.
- Taxon
- cucumberCucumis sativust0
- Treatment
- BAP (0.7 and 0.9 mg/liter) with 2,4-D (0.75, 1 and 1.25 mg/L)e0
- Outcome
- percentage of callus formatione0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest percentage of callus formation was 100% in both cultivars in environments with the combination of BAP (0.7 and 0.9 mg/liter) with 2,4-D (0.75, 1 and 1.25 mg/L)”
t0“cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Sina also reached 100% callus formation with the stated 1.1 mg/L BAP combination.
- Taxon
- cucumberCucumis sativust0
- Material
- Sinae0
- Treatment
- BAP (1.1 mg/L) with 2,4-D (0.75, 1.1 and 1.25 mg/L)e0
- Outcome
- percentage of callus formatione0
- Direction
- higheste0
- Value
- 100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest percentage of callus formation was 100% in both cultivars in environments with the combination of BAP (0.7 and 0.9 mg/liter) with 2,4-D (0.75, 1 and 1.25 mg/L) and in Sina cultivar BAP (1.1 mg/L) with 2,4-D (0.75, 1.1 and 1.25 mg/L)”
t0“cucumber cultivars (Cucumis sativus L.)”
Claim · model-written summary
Nagin with 1 mg/liter 2,4-D had the lowest callus formation, averaging 33.333%.
- Taxon
- cucumberCucumis sativust0
- Material
- Nagine0
- Treatment
- 2,4-D hormone with a concentration of 1 mg/litere0
- Outcome
- percentage of callus formatione0
- Direction
- loweste0
- Value
- 33.333%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lowest percentage of callus formation was observed in Nagin cultivar in the presence of 2,4-D hormone with a concentration of 1 mg/liter with an average of 33.333%”
t0“cucumber cultivars (Cucumis sativus L.)”
The same questions in other papers
N-benzyladenine · growth · Cucumis sativus (cucumber)Results differ
29 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- negative
30 papers more in this group
See all 34 papers →2,4-D · growth · Cucumis sativus (cucumber)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
7 papers more in this group
See all 11 papers →class · growth · Cucumis sativus (cucumber)Positive and negative results
9 papers in the same direction as this paper · 22 in the opposite direction
- positive
- positive
- positive
28 papers more in this group
See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.