Abstract
The results of studies on the stimulation of soybean seed germination under the influence of nanopriming with a chemically synthesized nanocomposite (NC) based on manganese-containing nanoparticles and a water-soluble polysaccharide arabinogalactan are presented. The study was carried out on the phytopathosystem: soybean seeds Glycine max (L) – phytopathogenic bacterium Pectobacterium carotovorum. Phytopathogenic bacterium P. carotovorum causes the infectious disease soft rot in various plant species from vegetables to trees and shrubs. After nanopriming (treatment Mn(OH)2/AGs NC), seed germination, biometric characteristics (mass and length of the root and hypocotyl) and biochemical parameters of soybean seedlings (content of reactive oxygen species (ROS), activity of antioxidant enzymes, amount of lipid peroxidation products) were analyzed. It was shown that the infection of seeds with P. carotovorum led to a decrease in their germination, 50 % of the seeds did not germinate. In addition, seedlings obtained from infected seeds had reduced all biometric characteristics compared to the control. Infection of seeds with P. carotovorum showed an increase in the amount of ROS in root tissues and caused a significant increase in peroxidase activity in the roots of soybean seedlings. A significant increase in diene conjugates (DC) was noted in the tissues of the roots of the hypocotel in this variant. The nanocomposite of manganese hydroxide and sulfated arabinogalactan (Mn(OH)2/AGs NC, 4,8 % Mn) removed the negative effect of the phytopathogen P. carotovorum. Morphometric characteristics of seed sprouts after nanopriming were characterized by an increase in biomass compared to the control. Thus, after nanopriming with Mn(OH)2/AGs NC, the length and weight of the root in uninfected seeds increased. The most pronounced stimulating effect of nanopriming was expressed in sprouts obtained from infected seeds. The length weight of the hypocotyl and the length of the root in infected seeds significantly increased after nanopriming of infected seeds. The precursor NC – MnSO4×5H2O did not affect the germination and sprouting of seeds both infected with P. carotovorum and free from infection. Nanopriming of infected seeds reduced the formation of ROS in seedling tissues. It was found that after nanopriming, peroxidase activity increased significantly in soybean roots compared to the control. This effect can be explained by the antioxidant effect of Mn NPs. Nanopriming of infected seeds reduced the amount of DC in root tissues to the control level. Thus, the mechanisms of interaction of NC with the studied phytopathosystem are represented by an increase in seed germination under the influence of NC, stimulation of morphometric parameters of seedlings, as well as an effect on the components of the antioxidant system. The obtained results and our previous studies on the antibacterial effect of Mn(OH)2/AGs against phytopathogens Clavibacter sepedonicus demonstrate the great potential of using Mn(OH)2/AGs in practical agriculture, in particular for application in the field of stimulating plant growth and increasing their resistance to phytopathogens.
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Claims 14
Claim · model-written summary
Infection cut germination, with half the seeds failing to germinate.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- infection of seeds with P. carotovorume0
- Interactor
- P. carotovorum
Pectobacterium carotovorume0pathogen - Outcome
- germinatione0
- Direction
- decreasee0
- Value
- 50 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infection of seeds with P. carotovorum led to a decrease in their germination, 50 % of the seeds did not germinate”
t0“Soybean”
Claim · model-written summary
Infection reduced seedling biometric traits.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- infected seedse0
- Outcome
- all biometric characteristicse0
- Direction
- reducede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seedlings obtained from infected seeds had reduced all biometric characteristics compared to the control”
t0“Soybean”
Claim · model-written summary
Infection increased root reactive oxygen species.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Infection of seeds with P. carotovorume0
- Interactor
- P. carotovorumPectobacterium carotovorume0pathogen
- Outcome
- amount of ROS in root tissuese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection of seeds with P. carotovorum showed an increase in the amount of ROS in root tissues”
t0“Soybean”
Claim · model-written summary
Infection significantly increased root peroxidase activity.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Infection of seeds with P. carotovorume0
- Interactor
- P. carotovorumPectobacterium carotovorume0pathogen
- Outcome
- peroxidase activity in the rootse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection of seeds with P. carotovorum showed an increase in the amount of ROS in root tissues and caused a significant increase in peroxidase activity in the roots of soybean seedlings”
Claim · model-written summary
Infection significantly increased diene conjugates.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- this variante0
- Outcome
- diene conjugates (DC)e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant increase in diene conjugates (DC) was noted in the tissues of the roots of the hypocotel in this variant”
t0“Soybean”
Claim · model-written summary
Nanocomposite treatment removed the pathogen negative effect.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Mn(OH)2/AGs NC, 4,8 % Mne0
- Interactor
- P. carotovorumPectobacterium carotovorume0pathogen
- Outcome
- negative effect of the phytopathogen P. carotovorume0
- Direction
- removede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nanocomposite of manganese hydroxide and sulfated arabinogalactan (Mn(OH)2/AGs NC, 4,8 % Mn) removed the negative effect of the phytopathogen P. carotovorum”
t0“Soybean”
Claim · model-written summary
Nanopriming increased sprout biomass.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nanopriminge0
- Outcome
- biomasse0
- Direction
- increasee0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Morphometric characteristics of seed sprouts after nanopriming were characterized by an increase in biomass compared to the control”
t0“Soybean”
Claim · model-written summary
Nanopriming increased root length and weight in uninfected seeds.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nanopriming with Mn(OH)2/AGs NCe0
- Outcome
- length and weight of the roote0
- Direction
- increasede0
- Qualifier
- uninfected seedse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after nanopriming with Mn(OH)2/AGs NC, the length and weight of the root in uninfected seeds increased”
t0“Soybean”
Claim · model-written summary
Nanopriming significantly increased hypocotyl length and weight in infected seeds.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nanopriming of infected seedse0
- Outcome
- length weight of the hypocotyle0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The length weight of the hypocotyl and the length of the root in infected seeds significantly increased after nanopriming of infected seeds”
t0“Soybean”
Claim · model-written summary
Nanopriming significantly increased root length in infected seeds.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- nanopriming of infected seedse0
- Outcome
- length of the roote0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The length weight of the hypocotyl and the length of the root in infected seeds significantly increased after nanopriming of infected seeds”
t0“Soybean”
Claim · model-written summary
The precursor did not affect germination or sprouting.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- MnSO4×5H2Oe0
- Outcome
- germination and sprouting of seedse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“precursor NC – MnSO4×5H2O did not affect the germination and sprouting of seeds both infected with P. carotovorum and free from infection”
t0“Soybean”
Claim · model-written summary
Nanopriming infected seeds reduced seedling ROS.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Nanopriming of infected seedse0
- Outcome
- formation of ROS in seedling tissuese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nanopriming of infected seeds reduced the formation of ROS in seedling tissues”
t0“Soybean”
Claim · model-written summary
Nanopriming significantly increased root peroxidase.
- Taxon
- soybeanGlycine maxe0
- Treatment
- nanopriminge0
- Outcome
- peroxidase activitye0
- Direction
- increasede0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after nanopriming, peroxidase activity increased significantly in soybean roots compared to the control”
Claim · model-written summary
Nanopriming infected seeds reduced root diene conjugates to control level.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Nanopriming of infected seedse0
- Outcome
- amount of DC in root tissuese0
- Direction
- reducede0
- Comparator
- the control levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nanopriming of infected seeds reduced the amount of DC in root tissues to the control level”
t0“Soybean”
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See all 36 papers →8 of 11 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.