Abstract
Abstract. The aim of this original research was to obtain new knowledge during biotechnological operations for the recovery of grape plant material from viral infections for subsequent practical use. The in vitro plants of 18 grape varieties of the "Magarach" Institute were the objects of research. The plants in vitro of the grapes were obtained in the necessary and sufficient quantity for carrying out experiments on health improvement. The complex of technological operations for recovery of grape plant material from viruses was carried out, including thermotherapy, chemotherapy and electrotherapy. After the recovery procedures, molecular diagnostics was performed on the latent form of phytopathogens in plant material. Viruses in plant material of the Alminskiy variety were completely eliminated. The infection of the Grapevine fanleaf virus and Grapevine rupestris stem pitting-associated virus has been eliminated or significantly reduced in the samples. The infection rate of Grapevine rupestris stem pitting-associated virus in plant material has been lowered by increasing exposure of plants in vitro to high temperatures. The use of ribavirin to eliminate the Grapevine rupestris stem pitting-associated virus should also be considered successful. The decrease in the infection rate of Grapevine rupestris stem pitting-associated virus in plant material compared to the control option resulting from electrotherapy is noteworthy. The use of biotechnological techniques has been demonstrated in experiments to eliminate or partially eliminate a number of viruses. The knowledge was obtained allows to optimize the recovery of grape genotypes from viruses in biotechnological systems. Samples of grape varieties that are free from latent infections should be used to replenish the vegetative collection in vitro of grapes created earlier at the Institute "Magarach". Keywords: biotechnology, in vitro, testing, thermotherapy, chemotherapy, electrotherapy
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Claims 7
Claim · model-written summary
Viruses were completely eliminated from Alminskiy grape material after recovery procedures.
- Taxon
- grapee1
- Material
- Alminskiye0
- Treatment
- thermotherapy, chemotherapy and electrotherapye1
- Outcome
- Viruses in plant materiale0
- Direction
- completely eliminatede0
Not reportedComparator
Verbatim
e0“Viruses in plant material of the Alminskiy variety were completely eliminated.”
e1“The complex of technological operations for recovery of grape plant material from viruses was carried out, including thermotherapy, chemotherapy and electrotherapy.”
Claim · model-written summary
Grapevine fanleaf virus infection was eliminated or significantly reduced in samples.
- Taxon
- grapet0
- Interactor
- Grapevine fanleaf virusGrapevine fanleaf viruse0pathogen
- Outcome
- infection of the Grapevine fanleaf viruse0
- Direction
- eliminated or significantly reducede0
Verbatim
e0“The infection of the Grapevine fanleaf virus and Grapevine rupestris stem pitting-associated virus has been eliminated or significantly reduced in the samples.”
t0“grape plant material”
Claim · model-written summary
Grapevine rupestris stem pitting-associated virus infection was eliminated or significantly reduced in samples.
- Taxon
- grapet0
- Interactor
- Grapevine rupestris stem pitting-associated virusGrapevine rupestris stem pitting-associated viruse0pathogen
- Outcome
- infection of the Grapevine fanleaf virus and Grapevine rupestris stem pitting-associated viruse0
- Direction
- eliminated or significantly reducede0
Verbatim
e0“The infection of the Grapevine fanleaf virus and Grapevine rupestris stem pitting-associated virus has been eliminated or significantly reduced in the samples.”
t0“grape plant material”
Claim · model-written summary
Longer high-temperature exposure lowered Grapevine rupestris stem pitting-associated virus infection rate.
- Taxon
- grapet0
- Treatment
- increasing exposure of plants in vitro to high temperaturese0
- Interactor
- Grapevine rupestris stem pitting-associated virusGrapevine rupestris stem pitting-associated viruse0pathogen
- Outcome
- infection rate of Grapevine rupestris stem pitting-associated viruse0
- Direction
- lowerede0
Not reportedComparator
Verbatim
e0“The infection rate of Grapevine rupestris stem pitting-associated virus in plant material has been lowered by increasing exposure of plants in vitro to high temperatures.”
t0“grape plant material”
Claim · model-written summary
Ribavirin was successful in eliminating Grapevine rupestris stem pitting-associated virus.
- Taxon
- grapet0
- Treatment
- ribavirine0
- Interactor
- Grapevine rupestris stem pitting-associated virusGrapevine rupestris stem pitting-associated viruse0pathogen
- Outcome
- Grapevine rupestris stem pitting-associated viruse0
- Direction
- eliminatee0
Not reportedComparator
Verbatim
e0“The use of ribavirin to eliminate the Grapevine rupestris stem pitting-associated virus should also be considered successful.”
t0“grape plant material”
Claim · model-written summary
Electrotherapy decreased Grapevine rupestris stem pitting-associated virus infection compared with control.
Verbatim
e0“The decrease in the infection rate of Grapevine rupestris stem pitting-associated virus in plant material compared to the control option resulting from electrotherapy is noteworthy.”
t0“grape plant material”
Claim · model-written summary
The authors recommend virus-free grape varieties for replenishing the institute collection.
Verbatim
e0“Samples of grape varieties that are free from latent infections should be used to replenish the vegetative collection in vitro of grapes created earlier at the Institute "Magarach".”
The same questions in other papers
ribavirin · Grapevine rupestris stem pitting-associated virus · VitisResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.