Abstract
Maintaining grape cultivation requires the continuous production of healthy propagating material and the control of economically important viruses. For more effective virus eradication, it is beneficial to try chemotherapeutic agents that have not yet been used on grapevine. We therefore tested two chemotherapeutic agents with different mechanisms of action (2-thiouracil and zidovudine) in addition to ribavirin, which is already used on grapevine. Viruses and viroids were identified in the mother plants of different grapevine cultivars by small RNA HTS (High-Throughput Sequencing), and RT-PCR verified the results. After chemotherapy, the regenerated plants were tested using RT-PCR and the efficacy of the three chemotherapeutic compounds was evaluated. Among the tested agents, ribavirin had the broadest elimination effect (the virus was not detectable by RT-PCR after 8 months) on most viruses. It proved to be particularly effective (the virus was not detectable by RT-PCR in most of the tested plants) against GFkV, GLRaV-4, GPGV and GRSPaV. The use of 2-thiouracil caused high phytotoxicity and was effective against GLRaV-4 among the viruses tested, having no effect on the other viruses. Zidovudine alone failed to eliminate any of the viruses we tested. 2-thiouracil, ribavirin and zidovudine were unable to eliminate HSVd and GYSVd-1 viroids.
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Claims 11
Claim · model-written summary
Ribavirin had the broadest virus elimination effect after eight months.
- Treatment
- ribavirine0
- Outcome
- virus was not detectable by RT-PCRe0
- Direction
- broadest elimination effecte0
- Qualifier
- after 8 monthse0
- Comparator
- tested agentse0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“Among the tested agents, ribavirin had the broadest elimination effect (the virus was not detectable by RT-PCR after 8 months) on most viruses”
Claim · model-written summary
Ribavirin eliminated GFkV from most tested grapevine plants.
- Treatment
- ribavirine1
- Interactor
- GFkVGrapevine fleck viruse0pathogen
- Outcome
- virus was not detectable by RT-PCRe0
- Direction
- particularly effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“It proved to be particularly effective (the virus was not detectable by RT-PCR in most of the tested plants) against GFkV, GLRaV-4, GPGV and GRSPaV”
e1“Among the tested agents, ribavirin had the broadest elimination effect”
Claim · model-written summary
Ribavirin eliminated GLRaV-4 from most tested grapevine plants.
- Treatment
- ribavirine0
- Interactor
- GLRaV-4e1pathogen
- Outcome
- virus was not detectable by RT-PCRe1
- Direction
- not detectablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“Among the tested agents, ribavirin had the broadest elimination effect”
e1“the virus was not detectable by RT-PCR in most of the tested plants) against GFkV, GLRaV-4, GPGV and GRSPaV”
Claim · model-written summary
Ribavirin eliminated GPGV from most tested grapevine plants.
- Treatment
- ribavirine0
- Interactor
- GPGVe1pathogen
- Outcome
- virus was not detectable by RT-PCRe1
- Direction
- not detectablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“Among the tested agents, ribavirin had the broadest elimination effect”
e1“the virus was not detectable by RT-PCR in most of the tested plants) against GFkV, GLRaV-4, GPGV and GRSPaV”
Claim · model-written summary
Ribavirin eliminated GRSPaV from most tested grapevine plants.
- Treatment
- ribavirine0
- Interactor
- GRSPaVGrapevine rupestris stem pitting-associated viruse1pathogen
- Outcome
- virus was not detectable by RT-PCRe1
- Direction
- not detectablee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“Among the tested agents, ribavirin had the broadest elimination effect”
e1“the virus was not detectable by RT-PCR in most of the tested plants) against GFkV, GLRaV-4, GPGV and GRSPaV”
Claim · model-written summary
2-thiouracil caused high phytotoxicity in grapevine.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“The use of 2-thiouracil caused high phytotoxicity”
Claim · model-written summary
2-thiouracil was effective against GLRaV-4.
- Treatment
- 2-thiouracile0
- Interactor
- GLRaV-4e0pathogen
- Outcome
- effective against GLRaV-4e0
- Direction
- effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“2-thiouracil caused high phytotoxicity and was effective against GLRaV-4 among the viruses tested”
Claim · model-written summary
2-thiouracil had no effect on other tested viruses.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“2-thiouracil caused high phytotoxicity and was effective against GLRaV-4 among the viruses tested, having no effect on the other viruses”
Claim · model-written summary
Zidovudine alone did not eliminate any tested viruses.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“Zidovudine alone failed to eliminate any of the viruses we tested”
Claim · model-written summary
None of the three agents eliminated HSVd.
- Treatment
- 2-thiouracil, ribavirin and zidovudinee0
- Interactor
- HSVdHop stunt viroide0pathogen
- Outcome
- eliminate HSVde0
- Direction
- unablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“2-thiouracil, ribavirin and zidovudine were unable to eliminate HSVd and GYSVd-1 viroids”
Claim · model-written summary
None of the three agents eliminated GYSVd-1.
- Treatment
- 2-thiouracil, ribavirin and zidovudinee0
- Interactor
- GYSVd-1e0pathogen
- Outcome
- eliminate HSVd and GYSVd-1 viroidse0
- Direction
- unablee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Grapevine”
e0“2-thiouracil, ribavirin and zidovudine were unable to eliminate HSVd and GYSVd-1 viroids”
The same questions in other papers
ribavirin · Grapevine rupestris stem pitting-associated virus · VitisResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.