Abstract
Several factors interact to regulate direct in vitro shoot regeneration. Optimization of de novo direct regeneration is a primary prerequisite for the success of genetic transformation experiments. However, achieving an optimized protocol is typically difficult due to the high cost and time consumption, as well as the complexity of this process. Hence, the application of computational techniques (machine learning (ML) algorithms) is essential for predicting de novo direct regeneration. This study examined the influence of various concentrations of optimal plant growth regulator (PGR) on the successful de novo in vitro regeneration of the shoot of Evolvulus alsinoides . The de novo direct regeneration of E. alsinoides was modelled using Multilayer Perceptron (MLP). Performance of the model was assessed using computational metrics (RMSE and R 2 ). The outcome demonstrated that the model algorithm had higher predictive accuracy. The mean square error (MSE) value was obtained as 5.18E-02, and the R 2 value was 0.99565. Moreover, the findings revealed a 90.83 % regeneration rate with 26.25 shoots per explant achieved from Murashige and Skoog (MS) medium supplemented with 2 μM Thidiazuron (TDZ) and Indole-3-acetic acid (IAA) (0.1 μM). Based on our results, the MLP was able to optimize the variables accurately. The results indicated good performance in modelling and optimization of in vitro de novo direct regeneration. The model may be used as a dependable and accurate predictive technique for ensuing investigations in in vitro plant genetic engineering. • In vitro regeneration of E. alsinoides plant was effectively developed. • MLP demonstrated best R 2 values and predicted shoot number, length and percentage regeneration. • The machine learning models prediction is in agreement with the experimental findings. • The ANN algorithms showed high prediction accuracy.
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Claims 5
Claim · model-written summary
The MLP model achieved a mean square error of 5.18E-02 in regeneration prediction.
- Taxon
- Evolvulus alsinoides
_in_Hyderabad_W_IMG_7978.jpg?width=60)
Evolvulus alsinoidest0 - Outcome
- mean square error (MSE)e0
- Value
- 5.18E-02e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_in_Hyderabad_W_IMG_7978.jpg?width=500)
Verbatim
e0“The mean square error (MSE) value was obtained as 5.18E-02”
t0“Evolvulus alsinoides”
Claim · model-written summary
The MLP model achieved R² of 0.99565.
- Taxon
- Evolvulus alsinoidesEvolvulus alsinoidest0
- Outcome
- R 2 valuee0
- Value
- 0.99565e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the R 2 value was 0.99565”
t0“Evolvulus alsinoides”
Claim · model-written summary
MS medium with TDZ and IAA achieved 90.83% regeneration.
- Taxon
- Evolvulus alsinoidesEvolvulus alsinoidest0
- Treatment
- Murashige and Skoog (MS) medium supplemented with 2 μM Thidiazuron (TDZ) and Indole-3-acetic acid (IAA) (0.1 μM)e0
- Outcome
- regeneration ratee0
- Value
- 90.83 %e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“90.83 % regeneration rate with 26.25 shoots per explant achieved from Murashige and Skoog (MS) medium supplemented with 2 μM Thidiazuron (TDZ) and Indole-3-acetic acid (IAA) (0.1 μM)”
t0“Evolvulus alsinoides”
Claim · model-written summary
The same medium produced 26.25 shoots per explant.
- Taxon
- Evolvulus alsinoidesEvolvulus alsinoidest0
- Treatment
- Murashige and Skoog (MS) medium supplemented with 2 μM Thidiazuron (TDZ) and Indole-3-acetic acid (IAA) (0.1 μM)e0
- Outcome
- shoots per explante0
- Value
- 26.25 shoots per explante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“90.83 % regeneration rate with 26.25 shoots per explant achieved from Murashige and Skoog (MS) medium supplemented with 2 μM Thidiazuron (TDZ) and Indole-3-acetic acid (IAA) (0.1 μM)”
t0“Evolvulus alsinoides”
Claim · model-written summary
The MLP predicted shoot number, length, and regeneration percentage accurately.
- Taxon
- Evolvulus alsinoidesEvolvulus alsinoidest0
- Outcome
- shoot number, length and percentage regeneratione0
Not reportedComparatorDirectionValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MLP demonstrated best R 2 values and predicted shoot number, length and percentage regeneration”
t0“Evolvulus alsinoides”