Paper · 2024 · Vestnik Altajskogo gosudarstvennogo agrarnogo universiteta

USE OF PROTEIN MARKERS IN PLANT BREEDING TO IMPROVE THE QUALITY OF BARLEY VARIETIES

Maksim Romakhin

15
claims
30
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

The research was carried out in order to increase the main indices of grain quality by the method of protein markers when developing varieties of the targeted use. Hordein polymorphism of 36 homogeneous accessions of competitive variety testing was studied by electrophoresis in starch gel. The study of the allelic composition of the hordein-coding loci of the breeding material revealed the variants A4, A23, B4, B164 and F3 clearly associated with low protein content parameters (10.8-11.5%) and high extractive substances (81.1-82.6%) in barley grain. The alleles A126, B21, B25, F1, associated with a higher protein level (12.6-13.3%), turned out to be valuable for feed purposes. Grain quality indices of universal use are associated with allelic variants A2, A23, B28, and F2. According to the frequency of allele occurrence, it was found that the main direction of selection in malting barley was in the direction of allele variants A23 (11.1%), B45 (8.3%), B164 (11.1%), F3 (22.2%). The forage varieties were characterized by a high frequency of A2 (22.2%), B25 (25.0%), F2 (27.8%) alleles, and universal varieties - A2 (22.2%), B8 (25.0%), F2 (22.2%) alleles. The formulas of hordein combining alleles characteristic of the best varieties of malting barley (A2 B45 F2; A2 B45F 3; A23 B164 F3; A4 B164 F3), universal barley (A2 B8 F2; A18 B8 F2; A23 B8 F2) and forage barley (A2 B25 F1) were identified.

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Claims 15

Associationobservedoriginal resultmeasured

Claim · model-written summary

Alleles A4, A23, B4, B164 and F3 are associated with low protein content parameters (10.8-11.5%) in barley grain.

Organ / stage
barley graine2
Gene
A4, A23, B4, B164 and F3e1
Outcome
protein content parameterse1
Direction
lowerinferrede1
Value
10.8-11.5%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“the variants A4, A23, B4, B164 and F3 clearly associated with low protein content parameters (10.8-11.5%) and high extractive substances (81.1-82.6%) in barley grain”

✓ located verbatim in the source · abstract, characters 389–554 · show in text ↑
e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Alleles A4, A23, B4, B164 and F3 are associated with high extractive substances (81.1-82.6%) in barley grain.

Organ / stage
barley graine2
Gene
A4, A23, B4, B164 and F3e1
Outcome
extractive substancese1
Direction
higherinferrede1
Value
81.1-82.6%e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“the variants A4, A23, B4, B164 and F3 clearly associated with low protein content parameters (10.8-11.5%) and high extractive substances (81.1-82.6%) in barley grain”

✓ located verbatim in the source · abstract, characters 389–554 · show in text ↑
e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Alleles A126, B21, B25 and F1 are associated with higher protein level (12.6-13.3%) and are valuable for feed purposes.

Gene
A126, B21, B25, F1e3
Outcome
protein levele3
Direction
highere3
Value
12.6-13.3%e3
Qualifier
valuable for feed purposese3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e3

“The alleles A126, B21, B25, F1, associated with a higher protein level (12.6-13.3%), turned out to be valuable for feed purposes”

✓ located verbatim in the source · abstract, characters 556–684 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Allelic variants A2, A23, B28 and F2 are associated with grain quality indices of universal use.

Gene
A2, A23, B28, and F2e4
Outcome
Grain quality indices of universal usee4

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e4

“Grain quality indices of universal use are associated with allelic variants A2, A23, B28, and F2”

✓ located verbatim in the source · abstract, characters 686–782 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele A23 occurred at 11.1% frequency in malting barley.

Material
malting barleye5
Gene
A23e5
Outcome
frequency of allele occurrencee5
Value
11.1%e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e5

“According to the frequency of allele occurrence, it was found that the main direction of selection in malting barley was in the direction of allele variants A23 (11.1%), B45 (8.3%), B164 (11.1%), F3 (22.2%)”

✓ located verbatim in the source · abstract, characters 784–990 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele B45 occurred at 8.3% frequency in malting barley.

Material
malting barleye5
Gene
B45e5
Outcome
frequency of allele occurrencee5
Value
8.3%e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e5

“According to the frequency of allele occurrence, it was found that the main direction of selection in malting barley was in the direction of allele variants A23 (11.1%), B45 (8.3%), B164 (11.1%), F3 (22.2%)”

✓ located verbatim in the source · abstract, characters 784–990 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele B164 occurred at 11.1% frequency in malting barley.

Material
malting barleye5
Gene
B164e5
Outcome
frequency of allele occurrencee5
Value
11.1%e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e5

“According to the frequency of allele occurrence, it was found that the main direction of selection in malting barley was in the direction of allele variants A23 (11.1%), B45 (8.3%), B164 (11.1%), F3 (22.2%)”

✓ located verbatim in the source · abstract, characters 784–990 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele F3 occurred at 22.2% frequency in malting barley.

Material
malting barleye5
Gene
F3e5
Outcome
frequency of allele occurrencee5
Value
22.2%e5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e5

“According to the frequency of allele occurrence, it was found that the main direction of selection in malting barley was in the direction of allele variants A23 (11.1%), B45 (8.3%), B164 (11.1%), F3 (22.2%)”

✓ located verbatim in the source · abstract, characters 784–990 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele A2 occurred at 22.2% frequency in forage varieties.

Material
forage varietiese6
Gene
A2e6
Outcome
frequencye6
Value
22.2%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e6

“The forage varieties were characterized by a high frequency of A2 (22.2%), B25 (25.0%), F2 (27.8%) alleles, and universal varieties - A2 (22.2%), B8 (25.0%), F2 (22.2%) alleles”

✓ located verbatim in the source · abstract, characters 992–1168 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele B25 occurred at 25.0% frequency in forage varieties.

Material
forage varietiese6
Gene
B25e6
Outcome
frequencye6
Value
25.0%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e6

“The forage varieties were characterized by a high frequency of A2 (22.2%), B25 (25.0%), F2 (27.8%) alleles, and universal varieties - A2 (22.2%), B8 (25.0%), F2 (22.2%) alleles”

✓ located verbatim in the source · abstract, characters 992–1168 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele F2 occurred at 27.8% frequency in forage varieties.

Material
forage varietiese6
Gene
F2e6
Outcome
frequencye6
Value
27.8%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e6

“The forage varieties were characterized by a high frequency of A2 (22.2%), B25 (25.0%), F2 (27.8%) alleles, and universal varieties - A2 (22.2%), B8 (25.0%), F2 (22.2%) alleles”

✓ located verbatim in the source · abstract, characters 992–1168 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele A2 occurred at 22.2% frequency in universal varieties.

Material
universal varietiese6
Gene
A2e6
Outcome
frequencye6
Value
22.2%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e6

“The forage varieties were characterized by a high frequency of A2 (22.2%), B25 (25.0%), F2 (27.8%) alleles, and universal varieties - A2 (22.2%), B8 (25.0%), F2 (22.2%) alleles”

✓ located verbatim in the source · abstract, characters 992–1168 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele B8 occurred at 25.0% frequency in universal varieties.

Material
universal varietiese6
Gene
B8e6
Outcome
frequencye6
Value
25.0%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e6

“The forage varieties were characterized by a high frequency of A2 (22.2%), B25 (25.0%), F2 (27.8%) alleles, and universal varieties - A2 (22.2%), B8 (25.0%), F2 (22.2%) alleles”

✓ located verbatim in the source · abstract, characters 992–1168 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Allele F2 occurred at 22.2% frequency in universal varieties.

Material
universal varietiese6
Gene
F2e6
Outcome
frequencye6
Value
22.2%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e6

“The forage varieties were characterized by a high frequency of A2 (22.2%), B25 (25.0%), F2 (27.8%) alleles, and universal varieties - A2 (22.2%), B8 (25.0%), F2 (22.2%) alleles”

✓ located verbatim in the source · abstract, characters 992–1168 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Hordein allele combinations characteristic of the best malting, universal and forage barley were identified.

Outcome
formulas of hordein combining allelese7
Qualifier
characteristic of the best varieties of malting barleye7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“in barley grain”

✓ located verbatim in the source · abstract, characters 539–554 · show in text ↑
e7

“The formulas of hordein combining alleles characteristic of the best varieties of malting barley (A2 B45 F2; A2 B45F 3; A23 B164 F3; A4 B164 F3), universal barley (A2 B8 F2; A18 B8 F2; A23 B8 F2) and forage barley (A2 B25 F1) were identified”

✓ located verbatim in the source · abstract, characters 1170–1411 · show in text ↑