Abstract
Successful pollination and fertilization are crucial for grain setting in cereals. Wheat is an allohexaploid autogamous species. Due to its evolutionary history, the genetic diversity of current bread wheat (Triticum aestivum) cultivars is limited. Introducing favorable alleles from related wild and cultivated wheat species is a promising breeding strategy for resolving this issue. However, wide hybridization between bread wheat and its relatives is hampered by the presence of suppressor genes and difficulties in crossing. Optimized methods for observing pollen tubes are essential for understanding the mechanism of crossability between wheat and its relatives. Here, we improved the crossing procedure between bread wheat and rye (Secale cereale) and established an optimized protocol for visualizing pollen tube behavior. Crossing via detached spike culture significantly enhanced crossing efficiency and phenotypic stability. A combination of canonical aniline blue staining and optimized clearing and sectioning allowed us to visualize pollen tube behavior. The proportion of rye pollen tubes reaching the micropyle was lower than that for pollen tubes germinated on the stigmatic hair, explaining why the hybrid seed-setting rate was approximately 75% instead of 100%. This method sheds light on wide hybridization through deeper visualization of the insides of pistils.
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Claims 4
Claim · model-written summary
Detached spike culture significantly improved crossing efficiency and phenotypic stability.
- Taxon
- Wheat
Triticum aestivumt0 - Treatment
- detached spike culturee0
- Outcome
- crossing efficiency and phenotypic stabilitye0
- Value
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Crossing via detached spike culture significantly enhanced crossing efficiency and phenotypic stability.”
t0“Wheat Pistils”
Claim · model-written summary
Aniline blue staining with clearing and sectioning allowed pollen tube behavior to be visualized.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- canonical aniline blue staining and optimized clearing and sectioninge0
- Outcome
- visualize pollen tube behaviore0
- Value
- allowede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A combination of canonical aniline blue staining and optimized clearing and sectioning allowed us to visualize pollen tube behavior.”
t0“Wheat Pistils”
Claim · model-written summary
A lower proportion of rye pollen tubes reached the micropyle than germinated on stigmatic hair.
- Taxon
- WheatTriticum aestivumt0
- Interactor
- rye
Secale cerealee0 - Outcome
- proportion of rye pollen tubes reaching the micropylee0
- Direction
- lowere0
- Comparator
- pollen tubes germinated on the stigmatic haire0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proportion of rye pollen tubes reaching the micropyle was lower than that for pollen tubes germinated on the stigmatic hair, explaining why the hybrid seed-setting rate was approximately 75% instead of 100%.”
t0“Wheat Pistils”
Claim · model-written summary
The hybrid seed setting rate was approximately 75% rather than 100%.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- hybrid seed-setting ratee0
- Value
- 75%e0
- Comparator
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hybrid seed-setting rate was approximately 75% instead of 100%.”
t0“Wheat Pistils”