Abstract
Immature embryos rescued in sunflower breeding programs represent one of the important procedures for creating a competitive research program. The development of inbred lines in a short period offers a significant advantage, enabling faster adaptation of sunflower hybrids to farmers’ needs, including new herbicide technologies, such as existing sulfonylurea- and imidazoline-based treatments or the introgression of new disease-resistant genes. In our research, we initiated immature embryo rescue by harvesting at 168 h, 216 h, 264 h, and 312 h after fecundations. The media used were based on MS (Murashige and Skoog) and supplemented with different concentrations of BAP, NAA, and GA3. For the experiments, three types of genotypes were used: inbred lines, populations, and sunflower hybrids. Our results demonstrated a significant connection between harvest timing, the medium used, and the genotype. The highest survival rates were recorded as up to 81% for hybrids and 71% for inbred lines. For immature embryo rescue, they were obtained at 312 h on MS5—2.0:0.4:0.4 (BAP:NAA:GA3). The genotype, culture medium composition, and harvesting time are essential factors for optimizing the in vitro rescue technique of immature sunflower embryos. The survival rates of immature embryos were significantly influenced by the interaction of these factors.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Embryo survival depended significantly on harvest time, culture medium, and genotype.
- Taxon
- Sunflower
Helianthus annuust0 - Material
- genotypee0
- Outcome
- significant connectione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower Inbred Line Development”
e0“Our results demonstrated a significant connection between harvest timing, the medium used, and the genotype”
Claim · model-written summary
Hybrid embryo survival reached up to 81%.
- Taxon
- SunflowerHelianthus annuust0
- Material
- hybridse0
- Outcome
- survival ratese0
- Direction
- higheste0
- Value
- up to 81%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower Inbred Line Development”
e0“highest survival rates were recorded as up to 81% for hybrids and 71% for inbred lines”
Claim · model-written summary
Inbred line embryo survival reached 71%.
- Taxon
- SunflowerHelianthus annuust0
- Material
- inbred linese0
- Outcome
- survival ratese0
- Direction
- higheste0
- Value
- 71%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower Inbred Line Development”
e0“highest survival rates were recorded as up to 81% for hybrids and 71% for inbred lines”
Claim · model-written summary
Best embryo survival was at 312 h on MS5 medium.
- Taxon
- SunflowerHelianthus annuust0
- Treatment
- MS5—2.0:0.4:0.4 (BAP:NAA:GA3)e0
- Outcome
- immature embryo rescuee0
- Value
- 312 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower Inbred Line Development”
e0“For immature embryo rescue, they were obtained at 312 h on MS5—2.0:0.4:0.4 (BAP:NAA:GA3)”
Claim · model-written summary
The interaction of genotype, medium and harvest time significantly influenced embryo survival.
- Taxon
- SunflowerHelianthus annuust0
- Material
- genotypee1
- Outcome
- survival rates of immature embryose0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sunflower Inbred Line Development”
e0“The survival rates of immature embryos were significantly influenced by the interaction of these factors”
e1“The genotype, culture medium composition, and harvesting time are essential factors”