Abstract
The monogenic resistance to powdery mildew (caused by Podosphaera leucotricha) of apple trees is crucial when selecting them. DNA markers allow it to differentiate apple cultivars by individual resistance traits and determine prospective genotypes with high reliability. The presented research determines the results of molecular genetics and phytopathological analyses of apple cultivars on loci Pl-2, Pl-1, Pl-w, and Pl-d for reactions to powdery mildew. The molecular screening procedure involved using seven gene-specific markers (EM M01, EM M02, AT20-450, OPN18 SCAR, OPU02 SCAR, dr70F/dr339, and dr55F/dr336R). The resistance genes Pl-1, Pl-2, Pl-w, and Pl-d were amplified in 30 of the 34 research cultivars, with 8 Kazakh and 26 other country apple cultivars among the ones researched, according to the results of a molecular screening. Although resistance genes are still helpful for breeding, they are only recommended for use in extended pyramids of multiple resistant genes. Several cultivars are excellent candidates for additional breeding programs against powdery mildew and for pyramiding the Podosphaera leucotricha resistant genes in new cultivars.
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Claims 3
Claim · model-written summary
Resistance genes Pl-1, Pl-2, Pl-w and Pl-d were amplified in 30 of 34 apple cultivars.
- Taxon
- apple
Malus domesticae0 - Interactor
- Podosphaera leucotricha
Podosphaera leucotrichae0pathogen - Gene
- Pl-1, Pl-2, Pl-w, and Pl-de1
- Outcome
- amplifiede1
- Value
- 30 of the 34e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.


Verbatim
e0“powdery mildew (caused by Podosphaera leucotricha) of apple trees”
e1“The resistance genes Pl-1, Pl-2, Pl-w, and Pl-d were amplified in 30 of the 34 research cultivars”
Claim · model-written summary
Several apple cultivars are excellent candidates for additional breeding against powdery mildew.
- Taxon
- apple
Malus domesticae0 - Outcome
- excellent candidates for additional breeding programse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“powdery mildew (caused by Podosphaera leucotricha) of apple trees”
e2“Several cultivars are excellent candidates for additional breeding programs against powdery mildew”
Claim · model-written summary
Resistance genes are only recommended for use in extended pyramids of multiple resistant genes.
- Taxon
- apple
Malus domesticae0 - Outcome
- use in extended pyramids of multiple resistant genese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“powdery mildew (caused by Podosphaera leucotricha) of apple trees”
e3“they are only recommended for use in extended pyramids of multiple resistant genes”