Abstract
In this study, a rapid, efficient, and stable Agrobacterium -mediated genetic transformation system was successfully developed using stem slices of fig cultivar ‘117D’ as explants. In the Murashige and Skoog basal medium supplemented with 2 mg · L⁻ 1 thidiazuron (TDZ) and 0.05 mg · L⁻ 1 1-naphthaleneacetic acid (NAA), the highest shoot-induction rate was 53.33%, while the medium with a hormone ratio of 3 mg · L⁻ 1 TDZ and 0.05 mg · L⁻ 1 NAA achieved a maximum callus-induction rate of 78.89%. The optimal infection parameters for fig callus and stem slices were obtained by infecting thin cell layers (TCLs) with Agrobacterium tumefaciens K599 (OD 600 = 0.6) under vacuum for 10 min, and shaking at 120 r · min -1 , 28 °C for 30 min. Finally, transgenic callus and plants were identified through green fluorescent protein screening, β-glucuronidase staining, PCR analysis, and Western blot analysis, successfully obtaining positive FcMYB114 -overexpressing (OE) callus and three transgenic plants. Utilizing these FcMYB114 transgenic callus and plants allows us to gain deeper insights into their roles in the growth, development, and metabolism of fig. In the future, we aim to identify new regulatory factors and leverage this efficient transgenic technology to investigate the regulatory mechanisms of key molecules in fig. The advancement of fig regeneration and transgenic systems provides a valuable tool for validating genetic functions in fruit trees and enhancing the agronomic traits of fig.
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Claims 5
Claim · model-written summary
Medium with 2 mg/L TDZ and 0.05 mg/L NAA gave the highest shoot-induction rate (53.33%).
- Taxon
- Ficus carica
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=60)
Ficus caricat0 - Treatment
- thidiazuron (TDZ)e1
- Outcome
- shoot-induction ratee1
- Direction
- higheste1
- Value
- 53.33%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=500)
Verbatim
t0“fig (Ficus carica)”
e1“In the Murashige and Skoog basal medium supplemented with 2 mg · L⁻ 1 thidiazuron (TDZ) and 0.05 mg · L⁻ 1 1-naphthaleneacetic acid (NAA), the highest shoot-induction rate was 53.33%”
Claim · model-written summary
A hormone ratio of TDZ and NAA gave the maximum callus-induction rate (78.89%).
- Taxon
- Ficus caricaFicus caricat0
- Treatment
- hormone ratioe2
- Outcome
- callus-induction ratee2
- Direction
- maximume2
- Value
- 78.89%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“fig (Ficus carica)”
e2“the medium with a hormone ratio of 3 mg · L⁻ 1 TDZ and 0.05 mg · L⁻ 1 NAA achieved a maximum callus-induction rate of 78.89%”
Claim · model-written summary
Optimal Agrobacterium infection parameters for fig callus and stem slices were OD600 0.6 with 10 min vacuum infection.
- Taxon
- Ficus caricaFicus caricat0
- Treatment
- under vacuum for 10 mine3
- Interactor
- Agrobacterium tumefaciens K599
Agrobacterium tumefaciense3other - Outcome
- optimal infection parameters for fig callus and stem slicese3
- Value
- OD 600 = 0.6e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“fig (Ficus carica)”
e3“The optimal infection parameters for fig callus and stem slices were obtained by infecting thin cell layers (TCLs) with Agrobacterium tumefaciens K599 (OD 600 = 0.6) under vacuum for 10 min”
Claim · model-written summary
Transformation yielded three transgenic fig plants overexpressing FcMYB114.
- Taxon
- Ficus caricaFicus caricat0
- Gene
- FcMYB114e4
- Outcome
- three transgenic plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“fig (Ficus carica)”
e4“successfully obtaining positive FcMYB114 -overexpressing (OE) callus and three transgenic plants”
Claim · model-written summary
A rapid, efficient and stable Agrobacterium-mediated genetic transformation system was developed for fig.
- Taxon
- Ficus caricaFicus caricat0
- Outcome
- genetic transformation systeme5
- Qualifier
- rapid, efficient, and stablee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“fig (Ficus carica)”
e5“a rapid, efficient, and stable Agrobacterium -mediated genetic transformation system was successfully developed using stem slices of fig cultivar ‘117D’ as explants”
The same questions in other papers
Thidiazuron · growth · Ficus carica (common fig)Results agree
2 papers in the same direction as this paper
- positive
- positive
class · growth · Ficus carica (common fig)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.