Abstract
Fig is one of the most important fruit trees in Egypt. It used to constitute the major source of income for the inhabitants of the western north coast of Egypt. Since 1993 fig cultivations were threatened by a number of factors including virus, insect and mite infections. An efficient system for regeneration and transformation of the common fig Ficus carica L. cultivar Sultani (fresh consumption) was required to conserve fig cultivation in the area. The effect of different combinations of BA and NAA/2,4-D and kinetin on callus formation from leaf segments were studied. Results showed that the best medium for callus formation was MS supplemented with 2.0 mg/l 2,4-D and 0.2 mg/l kinetin. The best plantlet differentiation was obtained at concentrations of 30 mg/l 2iP and 7 mg/l TDZ with 0.25 mg/l NAA (with a regeneration efficiency of 83 and 79%, respectively). On the other hand, the obtained callus failed to induce organogenesis on media containing a combination of BA and kinetin. The highest shoot formation percentage (89%) was obtained when using 2 mg/l TDZ and 4 mg/l 2iP. The highest percentage of shoots forming roots (95%) was obtained when using MS medium supplemented with 1.0 mg/l IBA. Explants were transformed using Agrobacterium and microprojectile bombardment using the plasmid pISV2678 which harbors the gus-intron and bar genes. Results showed that the highest transformation efficiency using the Agrobacterium (17.5%) was obtained when explants were co-cultivated with the bacteria for 30 min. The highest transformation efficiency recorded using the microprojectile bombardment (12%) was obtained with 2.0 μg DNA per shot at 1,100 psi and a distance of 6 cm repeated twice. The transgenic nature of regenerated plants was confirmed by PCR analysis, histochemical GUS assay and leaf painting assay.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
The best callus formation from fig leaf segments was on MS supplemented with 2.0 mg/l 2,4-D and 0.2 mg/l kinetin.
- Taxon
- Ficus carica L.
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=60)
Ficus caricae0 - Material
- Sultanie0
- Treatment
- MS supplemented with 2.0 mg/l 2,4-D and 0.2 mg/l kinetine1
- Outcome
- callus formatione1
- Direction
- beste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=500)
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“the best medium for callus formation was MS supplemented with 2.0 mg/l 2,4-D and 0.2 mg/l kinetin”
Claim · model-written summary
The best plantlet differentiation, with regeneration efficiencies of 83 and 79%, was obtained at 30 mg/l 2iP and 7 mg/l TDZ with 0.25 mg/l NAA.
- Taxon
- Ficus carica L.Ficus caricae0
- Material
- Sultanie0
- Treatment
- 30 mg/l 2iP and 7 mg/l TDZ with 0.25 mg/l NAAe1
- Outcome
- plantlet differentiatione1
- Direction
- beste1
- Value
- 83 and 79%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“The best plantlet differentiation was obtained at concentrations of 30 mg/l 2iP and 7 mg/l TDZ with 0.25 mg/l NAA (with a regeneration efficiency of 83 and 79%, respectively)”
Claim · model-written summary
Fig callus failed to induce organogenesis on media containing a combination of BA and kinetin.
- Taxon
- Ficus carica L.Ficus caricae0
- Material
- Sultanie0
- Treatment
- combination of BA and kinetine1
- Outcome
- organogenesise1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“the obtained callus failed to induce organogenesis on media containing a combination of BA and kinetin”
Claim · model-written summary
The highest shoot formation percentage in fig, 89%, was obtained with 2 mg/l TDZ and 4 mg/l 2iP.
- Taxon
- Ficus carica L.Ficus caricae0
- Material
- Sultanie0
- Treatment
- 2 mg/l TDZ and 4 mg/l 2iPe1
- Outcome
- shoot formation percentagee1
- Direction
- higheste1
- Value
- 89%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“The highest shoot formation percentage (89%) was obtained when using 2 mg/l TDZ and 4 mg/l 2iP”
Claim · model-written summary
The highest percentage of fig shoots forming roots, 95%, was obtained on MS medium supplemented with 1.0 mg/l IBA.
- Taxon
- Ficus carica L.Ficus caricae0
- Material
- Sultanie0
- Treatment
- MS medium supplemented with 1.0 mg/l IBAe1
- Outcome
- percentage of shoots forming rootse1
- Direction
- higheste1
- Value
- 95%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“The highest percentage of shoots forming roots (95%) was obtained when using MS medium supplemented with 1.0 mg/l IBA”
Claim · model-written summary
The highest Agrobacterium transformation efficiency of fig explants, 17.5%, was obtained with 30 min co-cultivation.
- Taxon
- Ficus carica L.Ficus caricae0
- Material
- Sultanie0
- Outcome
- transformation efficiencye1
- Direction
- higheste1
- Value
- 17.5%e1
- Qualifier
- co-cultivated with the bacteria for 30 mine1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“the highest transformation efficiency using the Agrobacterium (17.5%) was obtained when explants were co-cultivated with the bacteria for 30 min”
Claim · model-written summary
The highest microprojectile bombardment transformation efficiency, 12%, was obtained with 2.0 μg DNA per shot at 1,100 psi and a distance of 6 cm repeated twice.
- Taxon
- Ficus carica L.Ficus caricae0
- Material
- Sultanie0
- Outcome
- transformation efficiencye1
- Direction
- higheste1
- Value
- 12%e1
- Qualifier
- 2.0 μg DNA per shot at 1,100 psi and a distance of 6 cm repeated twicee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“The highest transformation efficiency recorded using the microprojectile bombardment (12%) was obtained with 2.0 μg DNA per shot at 1,100 psi and a distance of 6 cm repeated twice”
Claim · model-written summary
The transgenic nature of regenerated fig plants was confirmed by PCR analysis, histochemical GUS assay and leaf painting assay.
- Taxon
- Ficus carica L.Ficus caricae0
- Material
- Sultanie0
- Outcome
- transgenic nature of regenerated plants was confirmede1
- Qualifier
- PCR analysis, histochemical GUS assay and leaf painting assaye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ficus carica L. cultivar Sultani”
e1“The transgenic nature of regenerated plants was confirmed by PCR analysis, histochemical GUS assay and leaf painting assay”
The same questions in other papers
Thidiazuron · growth · Ficus carica (common fig)Results agree
2 papers in the same direction as this paper
- positive
- positive
2,4-D · growth · Ficus carica (common fig)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
kinetin · growth · Ficus carica (common fig)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.