Abstract
A system for regeneration and Agrobacferium mediated transformation of the common figFr'cus caries L. of the cultivar Sultani was developed. Different combinations of BA and NAA or 2,4-D and kinetin of callus formation from leaf segment explants. and the best % formation of callus was found to be 2.0 mg/L 2,4-D and 0.2 mgfL kinetin. Callus produced somatic embryogenesis which differentiated to plantlets on media containing TDZ in combination with NAA or 2ip.The best concentrations which produce somatic embryogenesis and differentiated to plantlets on media were containingSO mgfl 2ip and 7 mg/L TDZ with 0.25 mg/L NAA, which gave the regeneration efficiencies 83 and 79 of respectively.The callus failed to induce organogenesis on media containing BA and kinetin. The best concentrations of growth regulators to produce shoots formation by direct somatic embryogenesis with leaf explants were on media containing 2 mg/L TDZ and 4 mg/L 2ip, which gave the regeneration efficiencies (89%).The best results of shoots forming roots was reached the highest value 95% by using MS medium supplemented with 1.0 mg/ L IBA. Leaf explants of in w'tro propagated plants were co-cultivation with Agrcbacferium cell culture containing the binary plasmid plSV267B which harbor the gus-intron and bar genes, the results showed that the highest transformation efficiency was obtained when explants incubated for 30 min. with bacterial suspension (17.5%). The transgenic nature of regenerated plants was confirmed by histochemical GUS, leaf painting assay and PCB analysis. Keywords: Fig (Ficus canoe); Transformation; Callus; Regeneration; GUS; bar, Agrobacterium fumefaciens
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Claims 9
Claim · model-written summary
Leaf-segment callus formation of fig was best on 2.0 mg/L 2,4-D with 0.2 mg/L kinetin.
- Taxon
- Ficus carica
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=60)
Ficus caricat0 - Organ / stage
- leaf segment explantse2
- Treatment
- 2.0 mg/L 2,4-D and 0.2 mgfL kinetine1
- Outcome
- best % formation of calluse1
- Direction
- beste1
- Comparator
- BA and NAAe2
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Ficus_carica)_-_Cagliari%2C_Italy_2024-03-26.jpg?width=500)
Verbatim
t0“Ficus carica L.”
e1“and the best % formation of callus was found to be 2.0 mg/L 2,4-D and 0.2 mgfL kinetin”
e2“Different combinations of BA and NAA or 2,4-D and kinetin of callus formation from leaf segment explants”
Claim · model-written summary
Fig callus failed to induce organogenesis on media containing BA and kinetin.
- Taxon
- Ficus caricaFicus caricat0
- Organ / stage
- calluse3
- Treatment
- media containing BA and kinetine3
- Outcome
- organogenesise3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e3“The callus failed to induce organogenesis on media containing BA and kinetin”
Claim · model-written summary
Callus of fig produced somatic embryogenesis that differentiated to plantlets on media with TDZ combined with NAA or 2ip.
- Taxon
- Ficus caricaFicus caricat0
- Organ / stage
- Calluse4
- Treatment
- TDZ in combination with NAA or 2ipe4
- Outcome
- somatic embryogenesis which differentiated to plantletse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e4“Callus produced somatic embryogenesis which differentiated to plantlets on media containing TDZ in combination with NAA or 2ip”
Claim · model-written summary
The best media with 2ip, TDZ and NAA gave regeneration efficiencies of 83 and 79.
- Taxon
- Ficus caricaFicus caricat0
- Treatment
- SO mgfl 2ip and 7 mg/L TDZ with 0.25 mg/L NAAe5
- Outcome
- regeneration efficienciese5
- Value
- 83 and 79e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e5“The best concentrations which produce somatic embryogenesis and differentiated to plantlets on media were containingSO mgfl 2ip and 7 mg/L TDZ with 0.25 mg/L NAA, which gave the regeneration efficiencies 83 and 79 of respectively”
Claim · model-written summary
Direct somatic embryogenesis from leaf explants on 2 mg/L TDZ and 4 mg/L 2ip gave a regeneration efficiency of 89%.
- Taxon
- Ficus caricaFicus caricat0
- Organ / stage
- leaf explantse6
- Treatment
- 2 mg/L TDZ and 4 mg/L 2ipe6
- Outcome
- regeneration efficienciese6
- Value
- 89%e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e6“The best concentrations of growth regulators to produce shoots formation by direct somatic embryogenesis with leaf explants were on media containing 2 mg/L TDZ and 4 mg/L 2ip, which gave the regeneration efficiencies (89%)”
Claim · model-written summary
Shoots forming roots reached the highest value, 95%, on MS medium supplemented with 1.0 mg/L IBA.
- Taxon
- Ficus caricaFicus caricat0
- Treatment
- MS medium supplemented with 1.0 mg/ L IBAe7
- Outcome
- shoots forming rootse7
- Direction
- higheste7
- Value
- 95%e7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e7“The best results of shoots forming roots was reached the highest value 95% by using MS medium supplemented with 1.0 mg/ L IBA”
Claim · model-written summary
Explants incubated for 30 min with bacterial suspension gave the highest transformation efficiency, 17.5%.
- Taxon
- Ficus caricaFicus caricat0
- Treatment
- explants incubated for 30 mine7
- Outcome
- transformation efficiencye7
- Direction
- higheste7
- Value
- 17.5%e7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e7“the results showed that the highest transformation efficiency was obtained when explants incubated for 30 min. with bacterial suspension (17.5%)”
Claim · model-written summary
Regenerated fig plants were confirmed as transgenic by histochemical GUS, leaf painting and PCR-type analysis.
- Taxon
- Ficus caricaFicus caricat0
- Outcome
- transgenic nature of regenerated plantse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e7“The transgenic nature of regenerated plants was confirmed by histochemical GUS, leaf painting assay and PCB analysis”
Claim · model-written summary
A regeneration and Agrobacterium-mediated transformation system was developed for fig cultivar Sultani.
- Taxon
- Ficus caricaFicus caricat0
- Material
- Sultanie7
- Outcome
- system for regeneration and Agrobacferium mediated transformatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Ficus carica L.”
e7“A system for regeneration and Agrobacferium mediated transformation of the common figFr'cus caries L. of the cultivar Sultani was developed”
The same questions in other papers
2,4-D · growth · Ficus carica (common fig)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
kinetin · growth · Ficus carica (common fig)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.