Abstract
ABSTRACT Kidney vetch ( Anthyllis vulneraria L.) is an old medicinal plant mostly known for ethnomedicine. Nowadays, its herbal parts are only scarcely used in phytotherapy, whereas medicinal applications of roots have not been reported so far. In this study, we investigated ethyl acetate (EtOAc) extracts obtained by solvent partition of an acetone/water crude extract of A. vulneraria roots. Mainly prenylated chalcones, flavones, isoflavones, and cinnamic acid derivatives dominated the compound profile when the extracts were studied by HPLC–ESI–(HR)MS n and GC–MS analysis. Two main chalcones ( 23 and 24 ) were isolated from the EtOAc extracts by several consecutive preparative chromatographic steps. Based on 1D‐ and 2D‐NMR data 24 was assigned to the known compound kanzonol C, while 23 was identified as a novel chalcone. Moreover, antiproliferative activities of the EtOAc extract and of isolated chalcones were investigated in different liver, breast, and thyroid carcinoma cell lines. Interestingly, the EtOAc extract and 24 exhibited the most pronounced decrease of cell viability in the MDA‐MB468 breast carcinoma cell line with an IC 50 value of 5.97 µM. Two synthetic derivatives of 24 (hexahydro‐ and triacetyl kanzonol, 30 , 31 , respectively), 23 and isobavachalcone 18 were less active (IC 50 > 11.7 µM). Thus, the prenylated chalcones detected in A. vulneraria roots appear to be promising lead structures for further antiproliferative studies.
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Claims 5
Claim · model-written summary
The root extract profile was dominated by prenylated chalcones, flavones and isoflavones.
- Taxon
- Kidney vetch
Anthyllis vulnerariat0 - Organ / stage
- Rootst1
- Outcome
- prenylated chalcones, flavones, isoflavonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mainly prenylated chalcones, flavones, isoflavones, and cinnamic acid derivatives dominated the compound profile”
t0“Kidney Vetch (Anthyllis vulneraria L.)”
t1“Roots of Kidney Vetch”
Claim · model-written summary
Cinnamic acid derivatives also dominated the root extract profile.
- Taxon
- Kidney VetchAnthyllis vulnerariat0
- Organ / stage
- Rootst0
- Outcome
- cinnamic acid derivativese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cinnamic acid derivatives dominated the compound profile”
t0“Roots of Kidney Vetch”
Claim · model-written summary
Two main chalcones were isolated from the root extracts.
- Taxon
- Kidney VetchAnthyllis vulnerariat0
- Organ / stage
- Rootst0
- Outcome
- Two main chalcones ( 23 and 24 )e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two main chalcones ( 23 and 24 ) were isolated from the EtOAc extracts”
t0“Roots of Kidney Vetch”
Claim · model-written summary
Chalcone 24 was identified as kanzonol C.
- Taxon
- Kidney VetchAnthyllis vulnerariat0
- Organ / stage
- Rootst0
- Outcome
- kanzonol Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“24 was assigned to the known compound kanzonol C”
t0“Roots of Kidney Vetch”
Claim · model-written summary
Chalcone 23 was identified as novel.
- Taxon
- Kidney VetchAnthyllis vulnerariat0
- Organ / stage
- Rootst0
- Outcome
- novel chalconee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“23 was identified as a novel chalcone”
t0“Roots of Kidney Vetch”