Abstract
Background: The NAC transcription factor family of genes is one of the largest families of transcription factors in plants, playing important functions in plant growth and development, response to adversity stress, disease resistance, and hormone signaling. In this study, we identified the number of members of the Panax notoginseng NAC (PnNAC) gene family and conducted a comprehensive analysis of their physicochemical characteristics, chromosomal location, evolutionary features, and expression patterns both in different parts of the plant at different growth stages and in response to infection by Alternaria panax. Methods: The NAC gene family in P. notoginseng was identified using Hidden Markov Model (HMMER) and National Center of Biotechnology Information Conserved Domain Database (NCBI CDD), and their physicochemical properties were analyzed with Perl scripts. Phylogenetic relationships were determined using Clustal Omega and FastTree, and gene structures were visualized with an R script. Promoter regions were analyzed with PlantCARE, motifs with MEME and ggmotif, and transcriptome data were processed using Hical Indexing for Spliced Alignment of Transcripts (HISAT2) and HTseq. Results: This study identified 98 PnNAC genes in P. notoginseng, analyzed their characteristics (protein lengths 104–882 aa, molecular weights 11.78–100.20 kDa, isoelectric points 4.12–9.75), location (unevenly distributed on 12 chromosomes, no tandem repeats), evolution, and expression patterns (distinct in different parts, growth stages, and after A. panax infection). Conclusions: PnNAC plays an important role in the growth and development of P. notoginseng and in its response to A. panax. PnNAC could be a candidate gene for further research on and functional analysis of P. notoginseng disease resistance.
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Claims 10
Claim · model-written summary
The study identified 98 PnNAC genes in P. notoginseng.
- Taxon
- P. notoginseng
Panax notoginsenge0 - Gene
- PnNAC genese0
- Outcome
- PnNAC genese0
- Value
- 98e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study identified 98 PnNAC genes in P. notoginseng”
Claim · model-written summary
PnNAC protein lengths ranged from 104 to 882 aa.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Outcome
- protein lengthse0
- Value
- 104–882 aae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein lengths 104–882 aa”
t0“Panax notoginseng”
Claim · model-written summary
PnNAC molecular weights ranged from 11.78 to 100.20 kDa.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Outcome
- molecular weightse0
- Value
- 11.78–100.20 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“molecular weights 11.78–100.20 kDa”
t0“Panax notoginseng”
Claim · model-written summary
PnNAC isoelectric points ranged from 4.12 to 9.75.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Outcome
- isoelectric pointse0
- Value
- 4.12–9.75e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isoelectric points 4.12–9.75”
t0“Panax notoginseng”
Claim · model-written summary
PnNAC genes were unevenly distributed on 12 chromosomes.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Outcome
- unevenly distributed on 12 chromosomese0
- Value
- 12 chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“unevenly distributed on 12 chromosomes, no tandem repeats”
t0“Panax notoginseng”
Claim · model-written summary
No PnNAC tandem repeats were found.
- Taxon
- Panax notoginsengPanax notoginsengt0
- Outcome
- no tandem repeatse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no tandem repeats”
t0“Panax notoginseng”
Claim · model-written summary
PnNAC expression differed among plant parts and growth stages.
- Taxon
- Panax notoginsengPanax notoginsenge1
- Outcome
- expression patternse0
- Qualifier
- distinct in different parts, growth stagese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression patterns (distinct in different parts, growth stages, and after A. panax infection)”
e1“Panax notoginseng NAC (PnNAC) gene family”
Claim · model-written summary
PnNAC expression changed after A. panax infection.
- Taxon
- Panax notoginsengPanax notoginsenge1
- Interactor
- A. panaxAlternaria panaxe0pathogen
- Outcome
- expression patternse0
- Qualifier
- after A. panax infectione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression patterns (distinct in different parts, growth stages, and after A. panax infection)”
e1“Panax notoginseng NAC (PnNAC) gene family”
Claim · model-written summary
The authors interpret PnNAC as important for P. notoginseng growth, development, and response to A. panax.
- Taxon
- P. notoginsengPanax notoginsenge0
- Interactor
- A. panaxAlternaria panaxe0pathogen
- Gene
- PnNACe0
- Outcome
- growth and development of P. notoginseng and in its response to A. panaxe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PnNAC plays an important role in the growth and development of P. notoginseng and in its response to A. panax.”
Claim · model-written summary
PnNAC is proposed as a candidate for functional disease-resistance research.
- Taxon
- P. notoginsengPanax notoginsenge0
- Gene
- PnNACe0
- Outcome
- candidate gene for further research on and functional analysis of P. notoginseng disease resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PnNAC could be a candidate gene for further research on and functional analysis of P. notoginseng disease resistance.”