Abstract
Vitamin E is an essential micronutrient that is abundant in peanut seeds. However, the absence of a rapid and reliable method for determining its content has impeded advancements in peanut quality improvement. In this study, we developed an efficient ultra-performance liquid chromatography variable wavelength detector (UPLC-vwd) method for quantifying vitamin E content in peanut seeds, capable of detecting four tocopherols and four tocotrienols . Compared to traditional methods, this approach is simpler, more efficient, and highly accurate. By comparing two sample preparation techniques, it was found that slicing better reflects the true vitamin E content than grinding, as it minimizes losses caused by mechanical pressure. The method demonstrated robust stability and accuracy in both repeatability tests and spiked recovery tests, showing no significant differences compared to the national standard method. The study revealed that the cotyledons of peanuts are the primary storage site for vitamin E, being rich in γ- and α-tocopherols, which together account for over 90°% of the total vitamin E content. In contrast, the vitamin E content in the seed coat is considerably lower. Therefore, the optimized detection method minimizes seed coat interference during sample preparation, ensuring the accuracy of the results. In summary, the UPLC-vwd method developed in this study is a promising tool for determining vitamin E content and supports the improvement of peanut quality.
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Claims 9
Claim · model-written summary
UPLC-vwd detected four tocopherols and four tocotrienols in peanut seeds.
- Taxon
- peanut seeds
Arachis hypogaeat0 - Outcome
- detecting four tocopherols and four tocotrienolse0
- Value
- four tocopherols and four tocotrienolse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UPLC-vwd) method for quantifying vitamin E content in peanut seeds, capable of detecting four tocopherols and four tocotrienols”
t0“peanut seeds”
Claim · model-written summary
The method was simpler, more efficient and highly accurate relative to traditional methods.
- Taxon
- peanut seedsArachis hypogaeat0
- Outcome
- simpler, more efficient, and highly accuratee0
- Comparator
- traditional methodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to traditional methods, this approach is simpler, more efficient, and highly accurate.”
t0“peanut seeds”
Claim · model-written summary
Slicing reflected true seed vitamin E content better than grinding by reducing pressure losses.
- Taxon
- peanut seedsArachis hypogaeat0
- Outcome
- true vitamin E contente0
- Direction
- bettere0
- Comparator
- grindinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“slicing better reflects the true vitamin E content than grinding, as it minimizes losses caused by mechanical pressure.”
t0“peanut seeds”
Claim · model-written summary
Repeatability and spike recovery tests showed robust method stability and accuracy.
- Taxon
- peanut seedsArachis hypogaeat0
- Outcome
- robust stability and accuracye0
- Comparator
- repeatability tests and spiked recovery testse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The method demonstrated robust stability and accuracy in both repeatability tests and spiked recovery tests”
t0“peanut seeds”
Claim · model-written summary
Method results did not differ significantly from the national standard method.
- Taxon
- peanut seedsArachis hypogaeat0
- Outcome
- differencese0
- Direction
- no significant differencese0
- Comparator
- national standard methode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“showing no significant differences compared to the national standard method.”
t0“peanut seeds”
Claim · model-written summary
Peanut cotyledons were the main vitamin E storage site.
- Taxon
- peanutsArachis hypogaeae0
- Organ / stage
- cotyledonse0
- Outcome
- primary storage site for vitamin Ee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cotyledons of peanuts are the primary storage site for vitamin E”
Claim · model-written summary
Gamma and alpha tocopherols together comprised over 90°% of total vitamin E in cotyledons.
- Taxon
- peanut seedsArachis hypogaeat0
- Outcome
- γ- and α-tocopherolse0
- Value
- over 90°%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“being rich in γ- and α-tocopherols, which together account for over 90°% of the total vitamin E content.”
t0“peanut seeds”
Claim · model-written summary
Vitamin E content was much lower in the seed coat.
- Taxon
- peanut seedsArachis hypogaeat0
- Organ / stage
- seed coate0
- Outcome
- vitamin E contente0
- Direction
- considerably lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the vitamin E content in the seed coat is considerably lower.”
t0“peanut seeds”
Claim · model-written summary
The optimized detection method minimizes seed coat interference during preparation.
- Taxon
- peanut seedsArachis hypogaeat0
- Outcome
- seed coat interferencee0
- Direction
- minimizese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the optimized detection method minimizes seed coat interference during sample preparation, ensuring the accuracy of the results.”
t0“peanut seeds”