Paper · 2025 · Frontiers in Microbiology

Effects of different concentrations of Lactiplantibacillus plantarum and Bacillus licheniformis on silage fermentation parameter, chemical composition and microbial community of Pennisetum sinese

Qiong Chen; Baisheng Yu; Yanchen Zhu; et al.

14
claims
29
verbatim quotes
6
citations
2
research areas
✓
Open access · cc-by

Abstract

The purpose of the experiment was to study the effects of different concentrations of Lactiplantibacillus plantarum (LP) and Bacillus licheniformis (BL) on the quality of Pennisetum sinese (PS) silage. The experiment consisted of seven treatment groups. The control group did not use additives, and the experimental groups were added with LP or BL of 1 × 10 5 CFU/g fresh weight (FW), 1 × 10 6 CFU/g FW and 1 × 10 7 CFU/g FW, respectively. The nutritional value of Pennisetum sinese silage was comprehensively evaluated using CNCPS 6.5 system and 16sDNA sequencing technology. The results showed that the ammonia nitrogen content and pH of each experimental group were significantly lower than those of the control group ( p < 0.05). The starch content gradually decreased and the water-soluble carbohydrate (WSC) content increased in both LP and BL groups with the increase of addition concentration. The LP7 group could significantly increase the true protein content in protein ( p < 0.05), and CP in BL groups decreased gradually with the increase of concentration. Compared with the control group, the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) was significantly lower in LP7 group ( p < 0.05) and the ADF content was significantly lower in BL5 group ( p < 0.05). In addition, LP and BL were able to change the proportion of each component in CNCPS system for Pennisetum sinese silage. The use of LP and BL can reduce the relative abundance of harmful microorganisms in silage such as Sediminibacterium and Nitrospira , and significantly change the microbial community structure in silage ( p < 0.05). In conclusion, LP and BL have significant effects on silage quality and nutritional value. The nutritional value of Pennisetum sinese in LP groups showed a dose-dependent effect, and adding 1 × 10 7 CFU/g LP have the best effect in silage. The best effect was achieved by adding 1 × 10 5 CFU/g BL in BL groups, and the effect of LP7 group was better than that of BL5 group.

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Claims 14

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Ammonia nitrogen content and pH of every experimental group (LP or BL additive) were significantly lower than the control in Pennisetum sinese silage (p < 0.05).

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
each experimental groupe0
Outcome
ammonia nitrogen content and pHe0
Direction
lowere0
Comparator
the control groupe0

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“the ammonia nitrogen content and pH of each experimental group were significantly lower than those of the control group ( p < 0.05)”

✓ located verbatim in the source · abstract, characters 601–732 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Starch content gradually decreased in both LP and BL groups as the addition concentration increased in Pennisetum sinese silage.

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
increase of addition concentratione0
Outcome
starch contente0
Direction
decreasede0

Not reportedComparator

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“The starch content gradually decreased and the water-soluble carbohydrate (WSC) content increased in both LP and BL groups with the increase of addition concentration”

✓ located verbatim in the source · abstract, characters 734–900 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Water-soluble carbohydrate (WSC) content increased in both LP and BL groups as the addition concentration increased in Pennisetum sinese silage.

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
increase of addition concentratione0
Outcome
water-soluble carbohydrate (WSC) contente0
Direction
increasede0

Not reportedComparator

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“The starch content gradually decreased and the water-soluble carbohydrate (WSC) content increased in both LP and BL groups with the increase of addition concentration”

✓ located verbatim in the source · abstract, characters 734–900 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The LP7 group significantly increased the true protein content in protein of Pennisetum sinese silage (p < 0.05).

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
LP7 groupe0
Outcome
true protein content in proteine0
Direction
increasee0

Not reportedComparator

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“The LP7 group could significantly increase the true protein content in protein ( p < 0.05)”

✓ located verbatim in the source · abstract, characters 902–992 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Crude protein (CP) in BL groups decreased gradually as the concentration increased in Pennisetum sinese silage.

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
increase of concentratione0
Outcome
CPe0
Direction
decreasede0

Not reportedComparator

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“CP in BL groups decreased gradually with the increase of concentration”

✓ located verbatim in the source · abstract, characters 998–1068 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Compared with the control, NDF and ADF contents were significantly lower in the LP7 group (p < 0.05).

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
LP7 groupe0
Outcome
content of neutral detergent fiber (NDF) and acid detergent fiber (ADF)e0
Direction
lowere0
Comparator
the control groupe0

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“Compared with the control group, the content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) was significantly lower in LP7 group ( p < 0.05)”

✓ located verbatim in the source · abstract, characters 1070–1227 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

ADF content was significantly lower in the BL5 group than in the control (p < 0.05).

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
BL5 groupe0
Outcome
ADF contente0
Direction
lowere0

Not reportedComparator

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“the ADF content was significantly lower in BL5 group ( p < 0.05)”

✓ located verbatim in the source · abstract, characters 1232–1296 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

LP and BL changed the proportion of each component in the CNCPS system for Pennisetum sinese silage.

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
LP and BLe0
Outcome
proportion of each component in CNCPS systeme0

Not reportedComparatorDirection

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“LP and BL were able to change the proportion of each component in CNCPS system for Pennisetum sinese silage”

✓ located verbatim in the source · abstract, characters 1311–1418 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Using LP and BL reduced the relative abundance of harmful microorganisms such as Sediminibacterium and Nitrospira in silage.

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
use of LP and BLe0
Interactor
Sediminibacterium and NitrospiraNitrospiraSediminibacteriume0other
Outcome
relative abundance of harmful microorganismse0
Direction
reducee0

Not reportedComparator

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“The use of LP and BL can reduce the relative abundance of harmful microorganisms in silage such as Sediminibacterium and Nitrospira”

✓ located verbatim in the source · abstract, characters 1420–1551 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

LP and BL significantly changed the microbial community structure in Pennisetum sinese silage (p < 0.05).

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
LP and BLe1
Outcome
microbial community structuree0

Not reportedComparatorDirection

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“significantly change the microbial community structure in silage ( p < 0.05)”

✓ located verbatim in the source · abstract, characters 1558–1634 · show in text ↑
e1

“The use of LP and BL”

✓ located verbatim in the source · abstract, characters 1420–1440 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The nutritional value of Pennisetum sinese silage in LP groups showed a dose-dependent effect.

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
LP groupse0
Outcome
nutritional valuee0

Not reportedComparatorDirection

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“The nutritional value of Pennisetum sinese in LP groups showed a dose-dependent effect”

✓ located verbatim in the source · abstract, characters 1727–1813 · show in text ↑
Recommendationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Adding 1 × 10 7 CFU/g LP gave the best effect in silage.

Taxon
Pennisetum sinesePennisetum sineset0
Outcome
best effect in silagee0

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“adding 1 × 10 7 CFU/g LP have the best effect in silage”

✓ located verbatim in the source · abstract, characters 1819–1874 · show in text ↑
Recommendationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Among BL groups, the best effect was achieved by adding 1 × 10 5 CFU/g BL.

Taxon
Pennisetum sinesePennisetum sineset0
Outcome
best effecte0

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“The best effect was achieved by adding 1 × 10 5 CFU/g BL in BL groups”

✓ located verbatim in the source · abstract, characters 1876–1945 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The effect of the LP7 group was better than that of the BL5 group.

Taxon
Pennisetum sinesePennisetum sineset0
Treatment
LP7 groupe0
Outcome
effecte0
Direction
bettere0
Comparator
BL5 groupe0

Verbatim

t0

“Pennisetum sinese”

✓ located verbatim in the source · title, characters 178–195 · show in text ↑
e0

“the effect of LP7 group was better than that of BL5 group”

✓ located verbatim in the source · abstract, characters 1951–2008 · show in text ↑