Abstract
The production of carrot (D. carota L.) doubled haploids (DH) for the acceleration of this important vegetable crop breeding requires genome doubling of haploid regenerants. If spontaneous doubling does not occur, artificial chromosome doubling can be complicated by the lack of efficient genome-doubling protocols. We tested an antimitotic agent treatment of carrot at the embryo stage. It allowed us to produce and treat a large number of clonal carrot embryos (at least 30 embryos per treatment condition) in small volumes with minimal reagent amounts. We showed that 0.01–1 g/L colchicine did not perturb carrot development. Trifluralin showed no signs of toxicity at 0.001 and 0.01 g/L concentrations, but 0.1 g/L trifluralin reduced survival by 40% and delayed plantlet regeneration. We showed via DNA content flow cytometry that 0.01–1 g/L colchicine and 0.001–0.1 g/L trifluralin could double the carrot genome. The highest diploid percent was observed at 1 g/L colchicine (34%) and 0.1 g/L trifluralin (28%). The highest percent of diploids together with mixoploids (partial diploids) was at 0.01 and 0.1 g/L trifluralin (over 70%), followed by 1 g/L colchicine (56%). To our knowledge, this is the first report on trifluralin application for genome doubling in Apiaceae. In our study, we determined colchicine and trifluralin toxicity and doubling efficiency at different concentrations that can be used for carrot DH-line production and further improvement of genome doubling methods.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Trifluralin at 0.1 g/L reduced carrot embryo survival by 40%
- Taxon
- carrot
Daucus carotae1 - Treatment
- 0.1 g/L trifluraline3
- Outcome
- survivale3
- Direction
- reducede3
- Value
- 40%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e3“0.1 g/L trifluralin reduced survival by 40% and delayed plantlet regeneration”
Claim · model-written summary
Trifluralin at 0.1 g/L delayed carrot plantlet regeneration
- Taxon
- carrotDaucus carotae1
- Treatment
- 0.1 g/L trifluraline3
- Outcome
- plantlet regeneratione3
- Direction
- delayede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e3“0.1 g/L trifluralin reduced survival by 40% and delayed plantlet regeneration”
Claim · model-written summary
Colchicine at 0.01–1 g/L did not perturb carrot development
- Taxon
- carrotDaucus carotae1
- Treatment
- 0.01–1 g/L colchicinee2
- Outcome
- carrot developmente2
- Direction
- did not perturbe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e2“We showed that 0.01–1 g/L colchicine did not perturb carrot development”
Claim · model-written summary
Trifluralin at 0.001 and 0.01 g/L showed no signs of toxicity in carrot
- Taxon
- carrotDaucus carotae1
- Treatment
- 0.001 and 0.01 g/Le3
- Outcome
- toxicitye3
- Direction
- no signs ofe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e3“Trifluralin showed no signs of toxicity at 0.001 and 0.01 g/L concentrations”
Claim · model-written summary
Colchicine at 0.01–1 g/L and trifluralin at 0.001–0.1 g/L could double the carrot genome
- Taxon
- carrotDaucus carotae1
- Treatment
- 0.01–1 g/L colchicine and 0.001–0.1 g/L trifluraline4
- Outcome
- carrot genomee4
- Direction
- could doublee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e4“We showed via DNA content flow cytometry that 0.01–1 g/L colchicine and 0.001–0.1 g/L trifluralin could double the carrot genome”
Claim · model-written summary
The highest diploid percent in carrot (34%) was observed at 1 g/L colchicine
- Taxon
- carrotDaucus carotae1
- Treatment
- 1 g/L colchicinee5
- Outcome
- diploid percente5
- Direction
- higheste5
- Value
- 34%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e5“The highest diploid percent was observed at 1 g/L colchicine (34%) and 0.1 g/L trifluralin (28%)”
Claim · model-written summary
The highest diploid percent in carrot (28%) was observed at 0.1 g/L trifluralin
- Taxon
- carrotDaucus carotae1
- Treatment
- 0.1 g/L trifluraline5
- Outcome
- diploid percente5
- Direction
- higheste5
- Value
- 28%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e5“The highest diploid percent was observed at 1 g/L colchicine (34%) and 0.1 g/L trifluralin (28%)”
Claim · model-written summary
The highest percent of diploids together with mixoploids in carrot (over 70%) was at 0.01 and 0.1 g/L trifluralin
- Taxon
- carrotDaucus carotae1
- Treatment
- 0.01 and 0.1 g/L trifluraline6
- Outcome
- diploids together with mixoploidse6
- Direction
- higheste6
- Value
- over 70%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e6“The highest percent of diploids together with mixoploids (partial diploids) was at 0.01 and 0.1 g/L trifluralin (over 70%), followed by 1 g/L colchicine (56%)”
Claim · model-written summary
Diploids together with mixoploids in carrot reached 56% at 1 g/L colchicine
- Taxon
- carrotDaucus carotae1
- Treatment
- 1 g/L colchicinee6
- Outcome
- diploids together with mixoploidse6
- Value
- 56%e6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We tested an antimitotic agent treatment of carrot at the embryo stage”
e6“The highest percent of diploids together with mixoploids (partial diploids) was at 0.01 and 0.1 g/L trifluralin (over 70%), followed by 1 g/L colchicine (56%)”
The same questions in other papers
trifluralin · growth · Daucus carota (carrot)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.