Paper · 2025 · Plant Protection

Host plant resistance in lentil germplasm against Fusarium wilt and its in vitro management using fungicides, plant growth promoting rhizobacteria, and plant extracts

Muhammad Umar Shahbaz; Muhammad Ehetisham ul Haq; Muhammad Kamran; et al.

8
claims
16
verbatim quotes
0
citations
2
research areas
✓
Open access

Abstract

Lentil, locally known as “Masoor” and valued as the “poor man’s meat,” is a protein-rich food crop severely affected by wilt disease caused by Fusarium oxysporum, leading to yield losses of 10–100%. The present study was conducted to screen lentil germplasm for resistance to F. oxysporum under sick field conditions and to evaluate the efficacy of various chemicals and plant growth-promoting rhizobacteria (PGPRs) against F. oxysporum under laboratory conditions. A total of 164 lentil genotypes were screened in the sick field. The results showed that no genotype was highly resistant; however, four genotypes viz. LPP 21133, LPP 21135, LPP 22102, and LPP 22115 were found to be resistant. Four different fungicides were evaluated at concentrations of 50 ppm and 100 ppm using the poisoned food technique. Among them, only score (difenoconazole) and topas (penconazole) were found to be the most effective against F. oxysporum at both concentrations. Six PGPR strains were also tested using the poisoned food technique. Of these, Pseudomonas fluorescens and P. aeruginosa showed effectiveness against F. oxysporum. Moreover, five different plant extracts were evaluated for their antifungal activity. Among these, only olive and neem extracts demonstrated some level of fungal growth inhibition in vitro. In conclusion, the study identified four resistant lentil genotypes and found that difenoconazole, penconazole, Pseudomonas fluorescens, P. aeruginosa, and extracts of olive and neem were effective in suppressing F. oxysporum, offering promising options for its management.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

No screened lentil genotype was highly resistant to Fusarium wilt.

Taxon
lentilLens culinarise0
Outcome
no genotype was highly resistante1

Not reportedInteractorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“no genotype was highly resistant”

✓ located verbatim in the source · abstract, characters 555–587 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Four lentil genotypes were resistant.

Taxon
lentilLens culinarise0
Material
LPP 21133, LPP 21135, LPP 22102, and LPP 22115e1
Outcome
resistante1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“four genotypes viz. LPP 21133, LPP 21135, LPP 22102, and LPP 22115 were found to be resistant.”

✓ located verbatim in the source · abstract, characters 598–692 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Difenoconazole was among the most effective fungicides against F. oxysporum at both concentrations.

Taxon
lentilLens culinarise0
Treatment
score (difenoconazole)e1
Interactor
F. oxysporumFusarium oxysporume1pathogen
Outcome
effective against F. oxysporume1
Direction
most effectivee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“only score (difenoconazole) and topas (penconazole) were found to be the most effective against F. oxysporum at both concentrations.”

✓ located verbatim in the source · abstract, characters 821–953 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Penconazole was among the most effective fungicides against F. oxysporum at both concentrations.

Taxon
lentilLens culinarise0
Treatment
topas (penconazole)e1
Interactor
F. oxysporumFusarium oxysporume1pathogen
Outcome
effective against F. oxysporume1
Direction
most effectivee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“only score (difenoconazole) and topas (penconazole) were found to be the most effective against F. oxysporum at both concentrations.”

✓ located verbatim in the source · abstract, characters 821–953 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Pseudomonas fluorescens was effective against F. oxysporum.

Taxon
lentilLens culinarise0
Interactor
F. oxysporumFusarium oxysporume1pathogen
Second interactor
Pseudomonas fluorescensPseudomonas fluorescense1biocontrol_agent
Outcome
effectiveness against F. oxysporume1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗
Pseudomonas fluorescens
Pseudomonas fluorescens · Second interactorNinjatacoshell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“Pseudomonas fluorescens and P. aeruginosa showed effectiveness against F. oxysporum.”

✓ located verbatim in the source · abstract, characters 1033–1117 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

P. aeruginosa was effective against F. oxysporum.

Taxon
lentilLens culinarise0
Interactor
F. oxysporumFusarium oxysporume1pathogen
Second interactor
P. aeruginosaPseudomonas aeruginosae1biocontrol_agent
Outcome
effectiveness against F. oxysporume1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗
Pseudomonas aeruginosa
Pseudomonas aeruginosa · Second interactorCDC · Public domain · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“Pseudomonas fluorescens and P. aeruginosa showed effectiveness against F. oxysporum.”

✓ located verbatim in the source · abstract, characters 1033–1117 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Olive extract inhibited fungal growth in vitro to some extent.

Taxon
lentilLens culinarise0
Treatment
olivee1
Outcome
fungal growth inhibitione1
Setting
in vitroe1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“only olive and neem extracts demonstrated some level of fungal growth inhibition in vitro.”

✓ located verbatim in the source · abstract, characters 1217–1307 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Neem extract inhibited fungal growth in vitro to some extent.

Taxon
lentilLens culinarise0
Treatment
neem extractse1
Outcome
fungal growth inhibitione1
Setting
in vitroe1

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“lentil germplasm”

✓ located verbatim in the source · abstract, characters 241–257 · show in text ↑
e1

“only olive and neem extracts demonstrated some level of fungal growth inhibition in vitro.”

✓ located verbatim in the source · abstract, characters 1217–1307 · show in text ↑

Azadirachta indica · disease · Lens culinaris (lentil)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

botanical · disease · Lens culinaris (lentil)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.