Abstract
Fungal phytopathogens employ effector proteins and secondary metabolites to subvert host immunity. Effector proteins have attracted widespread interest in infection, especially for unknown, unreported genes. However, the type of protein remains much less explored. Here, we combined transcriptome analysis and functional validation to identify virulence-associated genes in Fusarium graminearum during fungi infection. A unique secreted protein, FGSE02, was significantly upregulated in the early infection stage. Proteomic characterization revealed that the protein contains a functional signal peptide but lacks known domains. The transient expression of FGSE02 in Nicotiana benthamiana induced rapid cell death, while gene knockout stains reduced fungal virulence without affecting growth. Our findings highlight FGSE02 as a key virulence factor, offering potential targets for disease control. Taken together, the results of this study identify a pathogenic factor and provide new insights into the development of green pesticides.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
FGSE02 was significantly upregulated in early Fusarium infection.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Gene
- FGSE02e0
- Outcome
- upregulatede0
- Direction
- upregulatede0
- Qualifier
- early infection stagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A unique secreted protein, FGSE02, was significantly upregulated in the early infection stage”
e1“Nicotiana benthamiana”
Claim · model-written summary
FGSE02 contains a functional signal peptide but lacks known domains.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae2
- Gene
- FGSE02t0
- Outcome
- functional signal peptidee0
- Qualifier
- lacks known domainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the protein contains a functional signal peptide but lacks known domains”
t0“FGSE02, a Novel”
e2“Nicotiana benthamiana”
Claim · model-written summary
Transient FGSE02 expression induced rapid cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- transient expression of FGSE02e0
- Gene
- FGSE02e0
- Outcome
- cell deathe0
- Direction
- inducede0
- Qualifier
- rapide0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transient expression of FGSE02 in Nicotiana benthamiana induced rapid cell death”
Claim · model-written summary
FGSE02 knockout reduced fungal virulence.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae2
- Treatment
- gene knockout stainse0
- Gene
- FGSE02t0
- Outcome
- fungal virulencee0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gene knockout stains reduced fungal virulence without affecting growth”
t0“FGSE02, a Novel”
e2“Nicotiana benthamiana”
Claim · model-written summary
FGSE02 knockout did not affect fungal growth.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae2
- Treatment
- gene knockout stainse0
- Gene
- FGSE02t0
- Outcome
- growthe0
- Direction
- without affectinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gene knockout stains reduced fungal virulence without affecting growth”
t0“FGSE02, a Novel”
e2“Nicotiana benthamiana”
The same questions in other papers
transgenic · cytology · Nicotiana benthamianaPositive and negative results
13 papers in the same direction as this paper · 28 in the opposite direction
- negative
- no effect
- negative
38 papers more in this group
See all 42 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.